Hypertonic saline solution decreases oxidative stress in liver hypothermic ischemia

Nunes, Giolana; Figueira, Estela Regina Ramos; Rocha-Filho, Joel Avancini; Lanchotte, Cinthia; Nacif, Lucas Souto; Ferreira, D.M.; Romano, Vitor Carminatti; Abdo, E. E.; D’Albuquerque, Luiz Augusto Carneiro; Galvão, Flávio Henrique Ferreira

doi:10.1016/j.surg.2020.12.042

Cited by 3 publications

(6 citation statements)

References 33 publications

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“…Our laboratory and others have used the present well‐established model of liver IR to study specifically liver ischemia without splanchnic vasculature obstruction and concomitant congestive bowel ischemia. 10 , 13 , 20 , 22 In this experiment, this model was tested after 4 h of reperfusion to which verify markers were involved in late immunological anti‐inflammatory effects of liver IR after the effect markers involved in the normal acute inflammatory phase. Interestingly, we observed that post‐conditioning with intravenous HFIP markedly attenuated late IR injury as measured by reduced serum transaminases, which represent the main markers indicating hepatocellular necrosis.…”

Section: Discussionmentioning

confidence: 99%

“… 10 Liver IR can induce increased serum concentrations of K + and lactate and cause a reduction in iCa 2 . 10 , 20 Also, glucose levels can vary within a wide range depending on the severity of liver injury. Figueira et al 13 showed profound hypoglycemia in a model of liver IR after 45 min of hepatic ischemia.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…After receiving standard intraperitoneal anesthesia with 100 mg/kg of 5% ketamine hydrochloride (Ketalar®, Cristália), and 10 mg/kg of 2% xylazine hydrochloride (Rompum®, Bayer), rats underwent standardized orotracheal intubation with Jelco® 18G and mechanical ventilation (Small Animal Ventilator model 683; Harvard Apparatus) with a tidal volume of 0.08 mL/g of body weight, respiratory rate of 60/min, and fraction of inspired oxygen/ (FiO 2 ) of 21%. 20 The right common carotid artery was catheterized with a polyethylene catheter (PE50) to record reperfusion hemodynamics and to conduct an arterial blood analysis. A median laparotomy extending for about 4 cm from the xiphoid appendix was performed.…”

Section: Methodsmentioning

confidence: 99%

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Hexafluoroisopropanol decreases liver ischemia–reperfusion injury by downregulation of high mobility group box‐1 protein

Moscoso

Figueira

Rocha-Filho

et al. 2022

Pharmacology Res & Perspec

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Liver ischemia–reperfusion (IR) injury is associated with poor outcome after liver transplantation and liver resections. Hexafluoroisopropanol (HFIP) is a tri‐fluorinated metabolites of volatile anesthetics and has modulatory effects on inflammation that have been observed mainly in cell culture experiments. In this survey, we investigated the effects of HFIP in a rat model of normothermic hepatic ischemia–reperfusion injury. Twenty‐four male Wistar rats were randomized into three groups: (1) control in which animals were submitted to 30 min of partial liver ischemia with resection of non‐ischemic liver lobes immediate after reperfusion, (2) pre‐ischemia (PI) group in which animals received intravenous HFIP (67 mg/kg) 5 min before liver ischemia, and (3) pre‐reperfusion (PR) group in which animals received intravenous HFIP (67 mg/kg) 5 min before reperfusion. Four hours after reperfusion, all animals were euthanized for sample collection. Aspartate and alanine transaminases, glucose, and high mobility group box‐1 (HMGB‐1) protein concentrations showed a significant decreased, and malondialdehyde was increased in the PR group compared with control and PI groups. Interleukin 6 (IL‐6) was increased in the PI group compared with control and PR groups. IL‐10 and ‐12 were increased in the PR and PI groups, respectively, when compared with the control group. Glucose decreased in the PR when compared with the control group. Post‐conditioning with HFIP led to a decrease in hepatocellular injury and was associated with a downregulation of HMGB‐1. The HFIP resulted in a better control of inflammatory response to ischemia–reperfusion even without causing a reduction in oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Hexafluoroisopropanol decreases liver ischemia–reperfusion injury by downregulation of high mobility group box‐1 protein

Moscoso

Figueira

Rocha-Filho

et al. 2022

Pharmacology Res & Perspec

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“…Os No presente estudo pretendemos demostrar que a administração de HFIP reduz a lesão de isquemia reperfusão, utilizando um modelo de isquemia parcial normotérmica em ratos bastante utilizado em nosso laboratório em estudos anteriores 43,[53][54][55][56][57][58] O HMGB-1 atua também como um importante mediador no eixo HMGB-1/TLR-4/NF-kB/citocinas pró-inflamatórias 31,63 . Estudos experimentais prévios de LIR hepática demostraram níveis aumentados dessa molécula logo após a reperfusão do fígado 32,33,63 .…”

Section: Análise Estatísticaunclassified

Efeitos do hexafluoro-2-propanol na lesão de isquemia e reperfusão hepática normotérmica em ratos

Moscoso¹

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À minha esposa, Sofía, minha parceira em tudo! Por seu apoio incondicional, seu suporte permanente, sua paciência ilimitada e por sua leveza... que me equilibra sempre. Além de sempre compreender a minha ausência na certeza do amanhã.Sem seu amor isso nunca seria possível.A minha família, pelo amor, pelos valores, pelos ensinamentos e pelo incentivo aos estudos, sempre com companheirismo e força nos momentos difíceis. Pilares do que sou, responsáveis pelos louros dos frutos que serão colhidos. AGRADECIMENTOSEsta tese de doutorado é uma grande conquista. É com grande alegria e satisfação que eu agradeço: À minha orientadora, Profa. Dra. Estela Regina Ramos Figueira, uma pessoa muito íntegra, e uma cientista ímpar e excepcional, que, sem seus conhecimentos e capacidades, esta tese não existiria. Obrigado por todos os ensinamentos, conhecimento e pela paciência comigo. A Sra. foi uma grande incentivadora e inspiradora nesses anos de Doutorado, sem sua ajuda seria muito mais difícil. Ao Prof. Dr. Joel Avancini Rocha Filho, pelo apoio no desenvolvimento deste projeto, pelos ensinamentos e pela paciência em todo meu processo de aprendizagem. Aos meus pais, Agustín e Susana, por todo o carinho, amor, incentivo, suporte e pela confiança que me dão e que tornam, assim, possível na minha vida que eu visualize e percorra os caminhos que eu escolho. Às minhas irmãs, Ximena e Cristina, por serem tão amigas e queridas, por serem exemplos de pessoas e por serem alicerces na minha vida. Agradeço ao meu cunhado, Rafael, que com seu talento e sensibilidade de sua arte, mesmo não sendo da área médica, conseguiu captar todas as minhas ideias e transmitir para as imagens dessa tese. À Valquíria Dias, Assessora Científica, pela revisão de Português e formatação desta tese, obrigado pelo seu profissionalismo e paciência. Ao Dr. Wellington Andraus, um professor e amigo, pela insistência em me mostrar que tudo isso vale a pena. Ao Dr. Lucas Nassif, pelo incentivo e pelas palavras e orientações tão engrandecedoras. À Comissão Examinadora de Exame de Qualificação, pelas valiosas orientações, contribuições, sugestões, que tornaram esta tese mais completa e grandiosa.

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The Effect of Ascorbic Acid on Hepatic Ischaemia–Reperfusion Injury in Wistar Rats: An Experimental Study

Ximenes,

Rocha-Filho,

Galvão

et al. 2024

IJMS

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Liver ischaemia–reperfusion (IR) during hepatic surgeries can lead to liver cell death via oxidative stress and the activation of immune cells, the release of cytokines, and damage-associated molecular patterns. Ascorbic acid has been shown to confer potential protective effects against IR injury, mainly due to its antioxidant properties. This study evaluated the effect of ascorbic acid infusion at different time points during hepatic IR in rats. Thirty-six male Wistar rats were divided into control and experimental groups that received the same total ascorbic acid dose at three different infusion times: before ischaemia, before reperfusion, or before both ischaemia and reperfusion. All of the animals experienced hepatic IR injury. We measured the hepatic enzymes, cytokines, and portal blood flow. Animals receiving ascorbic acid before both ischaemia and reperfusion had lower liver enzyme levels, reduced inflammation, and better portal venous flow than other animals. Divided doses of ascorbic acid before IR may be beneficial for reducing liver injury associated with IR.

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Hypertonic saline solution decreases oxidative stress in liver hypothermic ischemia

Cited by 3 publications

References 33 publications

Hexafluoroisopropanol decreases liver ischemia–reperfusion injury by downregulation of high mobility group box‐1 protein

Hexafluoroisopropanol decreases liver ischemia–reperfusion injury by downregulation of high mobility group box‐1 protein

Efeitos do hexafluoro-2-propanol na lesão de isquemia e reperfusão hepática normotérmica em ratos

The Effect of Ascorbic Acid on Hepatic Ischaemia–Reperfusion Injury in Wistar Rats: An Experimental Study

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