Hypertonic Xylose Agar Medium: A Novel Medium for Differentiation of Candida dubliniensis from Candida albicans

Jan, Abiroo; Bashir, Gulnaz; Fomda, Bashir Ahmad; Khangsar, Dekyong Angmo Urgain; Manzoor, Munazah; Kohli, Amrish; Reshi, Sulmaz; Suhail, Mohd; Choudhary, Saba; Fatima, Akeela

doi:10.4103/ijmm.ijmm_17_216

Cited by 6 publications

(2 citation statements)

References 15 publications

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“…All isolates show germ tube test and chlamydospores test positive on cornmeal agar and Tween 80 was identified as C. albicans or C. dubliniensis. Sunflower seed agar and xylose hypertonic media were used to differentiate both species [20].…”

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confidence: 99%

Efficacy and effect of Salvadora persica (Miswak), Toothbrush and Toothpaste on colonization of Oral Microbial Flora

2021

MRD

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Background: Various oral health measures have been applied throughout the world. The most common way is to use a toothbrush and toothpaste. However, the traditional way of using chewing sticks is found in several parts of the world. Chewing sticks have a mechanical cleansing action similar to a toothbrush in addition to their antimicrobial effect.Objective: To evaluate the efficacy of Salvadora persica (Miswak) products on oral microbial flora (bacteria and fungi) in comparison with toothbrushes and toothpaste. Method:A cross-sectional study was conducted on 60 randomly selected participants, during the period from March to August 2021. An oral rinses sample was collected at 2-time intervals: Samples were collected before use and after one week of use from two groups (a group used Miswak and group using ordinary toothpaste). The samples were immediately processed for microbiological phenotypic conventional methods and in vitro susceptibility testing of the microbial isolates to antimicrobial. Result:The organisms isolated from Miswak group, toothbrush and toothpaste group were identified. Among the bacterial isolates, Streptococci mutans was the most isolates 16 (53.33%) from Miswak group, and in 17 (56.67%) of toothbrush & toothpaste group, followed by Lactobacillus 12 (54%) in Miswak group and 10 (33.33%) toothbrush & toothpaste group. Staphylococci aureus was found in Miswak group 2 6.67%) and 3 10%) toothbrush & toothpaste groups respectively. Candida isolates was manly C. albicans 11 (36.67%) from Miswak group, and in 12 (40%) of toothbrush & toothpaste group, the second isolate was C. dubliniensis 2 (6.687%) Miswak group, and in 6 (20%) toothbrush & toothpaste followed by C. glabrata 1(3.33%) from Miswak group. Conclusion:Miswak has a significant reduction effect on both bacteria, candida immediately and after a week of use.

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Section: )mentioning

confidence: 99%

Efficacy and effect of Salvadora persica (Miswak), Toothbrush and Toothpaste on colonization of Oral Microbial Flora

2021

MRD

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“…However, C. dubliniensis is clinically a less common species among approximately 150 Candida species [ 3 ], accounting for 0.1–1% of Candida species isolated from humans. It is a representative isolate of cryptic Candida species, formerly considered C. albicans because of its phenotypical similarity in microscopic morphology and production of pseudohyphae, true hyphae, and chlamydospores [ 5 , 6 ]. Previous studies demonstrated that nearly 2–3% of C. dubliniensis isolates may be misidentified as C. albicans [ [7] , [8] , [9] , [10] ].…”

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confidence: 99%

Candida dubliniensis fungemia in a patient with severe COVID-19: A case report

Kakehi

Hagiya

Iio

et al. 2022

Journal of Infection and Chemotherapy

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Evaluation of various phenotypic methods for differentiation of Candida dubliniensis from Candida albicans

Jan

Bashir

Altaf

et al. 2022

Journal of Microbiological Methods

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Hypertonic Xylose Agar Medium: A Novel Medium for Differentiation of Candida dubliniensis from Candida albicans

Abstract: Hence, we propose HXAM as a sole phenotypic method for identifying C. dubliniensis from germ-tube-positive isolates or from stock collections of known C. albicans.

Cited by 6 publications

References 15 publications

Efficacy and effect of Salvadora persica (Miswak), Toothbrush and Toothpaste on colonization of Oral Microbial Flora

Efficacy and effect of Salvadora persica (Miswak), Toothbrush and Toothpaste on colonization of Oral Microbial Flora

Candida dubliniensis fungemia in a patient with severe COVID-19: A case report

Evaluation of various phenotypic methods for differentiation of Candida dubliniensis from Candida albicans

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