Hypertrehalosaemic peptides in the honeybee (Apis mellifera): purification, identification and function

Lorenz, Matthias; Kellner, Roland; Woodring, Joseph; Hoffmann, Klaus H.; GÄde, Gerd

doi:10.1016/s0022-1910(98)00158-9

Cited by 34 publications

(23 citation statements)

References 11 publications

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“…The role of AKH in social Hymenoptera is not well understood. In honeybees, AKH was in some studies either not detected (e.g., Veenstra et al, 2012) or only at low quantities in one subspecies, but not another (Lorenz et al, 1999; Woodring et al, 2003). Also, honeybees seem to not or hardly respond to AKH (Lorenz et al, 1999; Woodring et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…In honeybees, AKH was in some studies either not detected (e.g., Veenstra et al, 2012) or only at low quantities in one subspecies, but not another (Lorenz et al, 1999; Woodring et al, 2003). Also, honeybees seem to not or hardly respond to AKH (Lorenz et al, 1999; Woodring et al, 2003). Similarly, workers of B. terrestris and B. hortorum did not react to AKH injections (Lorenz et al, 2001), but all investigated bumblebee species contained AKH (Lorenz et al, 2001), and AKH genes are conserved across Hymenoptera (Veenstra et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Jedlička

Ernst

Votavová³

et al. 2016

Front. Physiol.

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Understanding the social evolution leading to insect eusociality requires, among other, a detailed insight into endocrine regulatory mechanisms that have been co-opted from solitary ancestors to play new roles in the complex life histories of eusocial species. Bumblebees represent well-suited models of a relatively primitive social organization standing on the mid-way to highly advanced eusociality and their queens undergo both, a solitary and a social phase, separated by winter diapause. In the present paper, we characterize the gene expression levels of major endocrine regulatory pathways across tissues, sexes, and life-stages of the buff-tailed bumblebee, Bombus terrestris, with special emphasis on critical stages of the queen's transition from solitary to social life. We focused on fundamental genes of three pathways: (1) Forkhead box protein O and insulin/insulin-like signaling, (2) Juvenile hormone (JH) signaling, and (3) Adipokinetic hormone signaling. Virgin queens were distinguished by higher expression of forkhead box protein O and downregulated insulin-like peptides and JH signaling, indicated by low expression of methyl farnesoate epoxidase (MFE) and transcription factor Krüppel homolog 1 (Kr-h1). Diapausing queens showed the expected downregulation of JH signaling in terms of low MFE and vitellogenin (Vg) expressions, but an unexpectedly high expression of Kr-h1. By contrast, reproducing queens revealed an upregulation of MFE and Vg together with insulin signaling. Surprisingly, the insulin growth factor 1 (IGF-1) turned out to be a queen-specific hormone. Workers exhibited an expression pattern of MFE and Vg similar to that of reproducing queens. Males were characterized by high Kr-h1 expression and low Vg level. The tissue comparison unveiled an unexpected resemblance between the fat body and hypopharyngeal glands across all investigated genes, sexes, and life stages.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Jedlička

Ernst

Votavová³

et al. 2016

Front. Physiol.

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“…In insects, the hypotrehalosaemic hormone stimulates degradation of glycogen and synthesis of trehalose in the fat body (Gäde 1999; Lorenz et al 1999). Likewise in other animals, the neuropeptide results in an increase of fructose-2,6-bisphosphate concentration, a compound responsible for the rate of glycolysis (Becker et al 1996; Meyer-Fernandes et al 2001).…”

Section: Introductionmentioning

confidence: 99%

The Activity of Carbohydrate-Degrading Enzymes in the Development of Brood and Newly Emerged workers and Drones of the Carniolan Honeybee,Apis mellifera carnica

Zoltowska

Lipiński²,

Łopieńska–Biernat

et al. 2012

Journal of Insect Science

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The activity of glycogen Phosphorylase and carbohydrate hydrolyzing enzymes α-amylase, glucoamylase, trehalase, and sucrase was studied in the development of the Carniolan honey bee, Apis mellifera carnica Pollman (Hymenoptera: Apidae), from newly hatched larva to freshly emerged imago of worker and drone. Phosphorolytic degradation of glycogen was significantly stronger than hydrolytic degradation in all developmental stages. Developmental profiles of hydrolase activity were similar in both sexes of brood; high activity was found in unsealed larvae, the lowest in prepupae followed by an increase in enzymatic activity. Especially intensive increases in activity occurred in the last stage of pupae and newly emerged imago. Besides α-amylase, the activities of other enzymes were higher in drone than in worker broods. Among drones, activity of glucoamylase was particularly high, ranging from around three times higher in the youngest larvae to 13 times higher in the oldest pupae. This confirms earlier suggestions about higher rates of metabolism in drone broods than in worker broods.

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“…Many AKHs have been isolated from cor pora cardiaca and characterized in a great number of insect orders (Gade et al, 1997;Gade, 1999;Lorenz et al, 1999), and recently also in the firebug Pyrrhocoris apterus, a representative species of the suborder Heterop tera (Kodrik et al, 2000). Almost all of these hormones are octa-, nona-or decapeptides with characteristic struc tural similarities (Gade et al, 1997), and they are grouped into the AKH/RPCH (Adipokinetic Hormone/Red Pig ment Concentrating Hormone) family.…”

Section: Introductionmentioning

confidence: 99%

Diel changes in adipokinetic response and walking activity of Pyrrhocoris apterus (Heteroptera) in relation to physiological status and wing dimorphism

Maxová¹,

Kodrı́k²,

Zemek³

et al. 2001

Eur. J. Entomol.

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An n a MAXOVÁ1-2, Da l ib o r KODRÍK1*2, Ro s t is l a v ZEMEK1 and Ra d o m ír SOCHA1 institute ofEntomology, Academy of Sciences, Branišovská 31, České Budějovice, 370 05, Czech Republic 2Faculty ofBiological Sciences, University of South Bohemia, Branišovská 31, České Budějovice, 370 05, Czech Republic Key words. Pyrrhocoris apterus, Pya-AKH, adipokinetic response, walking activity, diurnal rhythm, diapause, wing polymorphism Abstract. Three experimental groups of adult females of Pyrrhocoris apterus (reproductive brachypterous, diapausing brachypterous, and macropterous with non-diapause type of reproductive arrest) were analysed for their dose dependency of adipokinetic response, sensitivity of the fat body to Pya-AKH and locomotor activity during a 24 hour cycle. The greatest adipokinetic response (lipid elevation after hormonal treatment) was observed in macropterous, lower in diapausing brachypterous and the lowest in repro ductive brachypterous females. Macropterous females also exhibited the highest activity of walking, while the lowest locomotor activity was found in diapausing brachypters. Diel changes of adipokinetic response were recorded in all three experimental groups, with maximal response occurring during the day at 6-7 h after the onset of light. On the contrary, the minimal adipokinetic response was found in the night and early after the onset of light. No significant differences in diel rhythms were observed between groups exposed to various day lengths. Diel changes in locomotor activity were found only in reproductive brachypterous females and females of macropterous morph, and showed a diurnal rhythm with only one peak of activity during a 24 hour cycle. In the latter, the rhythmic pattern of walking activity was positively correlated with diel changes of adipokinetic response. This indicates an impor tant role of Pya-AKH in dispersal strategy of macropterous females of this heteropteran.

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Hypertrehalosaemic peptides in the honeybee (Apis mellifera): purification, identification and function

Cited by 34 publications

References 11 publications

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

The Activity of Carbohydrate-Degrading Enzymes in the Development of Brood and Newly Emerged workers and Drones of the Carniolan Honeybee,Apis mellifera carnica

Diel changes in adipokinetic response and walking activity of Pyrrhocoris apterus (Heteroptera) in relation to physiological status and wing dimorphism

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