Hyphal Structure

Roberson, Robert W.; Abril, Maritza; Blackwell, Meredith; Letcher, Peter M.; McLaughlin, David J.; Mouriño-Pérez, Rosa R.; Riquelme, Meritxell; Uchida, Maho

doi:10.1128/9781555816636.ch2

Cited by 16 publications

(25 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, the Spitzenkörper, a vesicle supply center that promotes and orients hyphal growth, is largely associated with Ascomycetes and Basidiomycetes septate hyphae, but is usually absent from earlydiverging fungal lineages. Instead, we find that S. japonicus hyphae accumulate secretory vesicles at the growing tip in a less clustered pattern, similar to what was observed in yeast growth and filamentous Zygomycetes species (Grove and Bracker, 1970;McClure et al, 1968;Roberson et al, 2010). Finally, the strict dependence of hyphae on actin-based transport and independent from microtubules also cast it apart from most other filamentous fungi, which use microtubules for long-range transport (Egan et al, 2012).…”

Section: A Mycelium Formed Of Single Cellssupporting

confidence: 78%

Yeast-to-hypha transition ofSchizosaccharomyces japonicusin response to natural stimuli

Kinnaer

Dudin

Martin

2018

Preprint

View full text Add to dashboard Cite

Many fungal species are dimorphic, exhibiting both unicellular yeast-like and filamentous forms. Schizosaccharomyces japonicus, a member of the fission yeast clade, is one such dimorphic fungus. Here, we first identify fruit extracts as natural, stress-free, starvation-independent inducers of filamentation, which we use to describe the properties of the dimorphic switch. During the yeast-tohypha transition, the cell evolves from a bipolar to a unipolar system with 10-fold accelerated polarized growth but constant width, vacuoles segregated to the non-growing half of the cell, and hyperlengthening of the cell. We demonstrate unusual features of S. japonicus hyphae: these cells lack a Spitzenkörper, a vesicle distribution center at the hyphal tip, but display more rapid cytoskeleton-based transport than the yeast form, with actin cables being essential for the transition. S. japonicus hyphae also remain mononuclear and undergo complete cell divisions, which are highly asymmetric: one daughter cell inherits the vacuole, the other the growing tip. We show these elongated cells scale their nuclear size, spindle length and elongation rates but display altered division size controls. This establishes S. japonicus as a unique system that switches between symmetric and asymmetric modes of growth and division.

show abstract

Section: A Mycelium Formed Of Single Cellssupporting

confidence: 78%

Yeast-to-hypha transition ofSchizosaccharomyces japonicusin response to natural stimuli

Kinnaer

Dudin

Martin

2018

Preprint

View full text Add to dashboard Cite

show abstract

“…Like the Dikarya, region I begins at the tip of the cell and typically extends back approximately 3–4 µm. Most members of the Mucoromycota and Zoopagomycota do not contain a Spk (Roberson et al ., 2010; Fisher & Roberson, 2016; Fisher et al ., 2018), but rather a thin crescent‐shaped band of secretory vesicles, the apical vesicle crescent (AVC), present just beneath the apical plasma membrane (Figs. 2B, 5) (Fisher & Roberson, 2016).…”

Section: Hyphal Tip Organisationmentioning

confidence: 99%

“…These vesicle aggregates are ephemeral and their presence is positively correlated with optimal hyphal growth (Girbardt, 1957; Riquelme et al ., 2016). Direction of hyphal growth is also correlated with Spk position and movements within the apical dome (Girbardt, 1957; Bartnicki‐García et al ., 1995; López‐Franco, & Bracker, 1996; Riquelme et al ., 1998; Roberson et al ., 2010; Fisher et al ., 2018;). This function for the AVC is not clear (Fisher & Roberson, 2016).…”

Section: The Hyphal Apexmentioning

confidence: 99%

“…However, due to their mode of growth and diverse interactions, hyphae contain subcellular characteristics that are distinctly fungal in nature. Characteristics of cytoplasmic order, apical vesicle organisation, cytoskeletal arrangement, microtubule organising centres (MtOC), and cellular compartmentalisation are of particular interest in studies of hyphal cell biology and fungal evolutionary relationships (Roberson et al ., 2010).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Subcellular structure and behaviour in fungal hyphae

Roberson

2020

Journal of Microscopy

View full text Add to dashboard Cite

This work briefly surveys the diversity of selected subcellular characteristics in hyphal tip cells of the fungal kingdom (Mycota). Hyphae are filamentous cells that grow by tip extension. It is a highly polarised mechanism that requires a robust secretory system for the delivery of materials (e.g. membrane, proteins, cell wall materials) to sites of cell growth. These events result it the self-assembly of a Spitzenkörper (Spk), found most often in the Basidiomycota, Ascomycota, and Blastocladiomycota, or an apical vesicle crescent (AVC), present in the most Mucoromycota and Zoopagomycota. The Spk is a complex apical body composed of secretory vesicles, cytoskeletal elements, and signaling proteins. The AVC appears less complex, though little is known of its composition other than secretory vesicles. Both bodies influence hyphal growth and morphogenesis. Other factors such as cytoskeletal functions, endocytosis, cytoplasmic flow, and turgor pressure are also important in sustaining hyphal growth. Clarifying subcellular structures, functions, and behaviours through bioimagining analysis are providing a better understanding of the cell biology and phylogenetic relationships of fungi.

show abstract

“…It seems reasonable that continued apical growth might require a more intense rate of protein production at this site. Indeed, across a range of filamentous fungi, ribosomes or rough endoplasmic reticulum can be observed in extreme apical regions (Roberson et al, 2010). For instance, a subtending mass of ribosomes has been observed in the spitzenkörper of Fusarium acuminatu (Howard, 1981).…”

Section: The Switch To Filamentous Growth Is Also Associated With Mrnmentioning

confidence: 99%

Translation factor mRNA granules direct protein synthetic capacity to regions of polarized growth

Pizzinga

Bates

Lui

et al. 2018

Preprint

View full text Add to dashboard Cite

Running title: Translation factor mRNA localization and inheritance SummaryThis study shows that mRNAs encoding a range of translation factors are localized to granules that get transported into the yeast daughter cell using the She2p/She3p machinery. This likely supports an intensification of protein synthetic activity to facilitate apical polarized growth.Abstract mRNA localization serves key functions in localized protein production making it critical that the translation machinery itself is present at these locations. Here we show that translation factor mRNAs are localized to distinct granules within yeast cells. In contrast to many mRNP granules, such as P-bodies and stress granules, which contain translationally repressed mRNAs, these granules harbor translated mRNAs under active growth conditions. The granules require Pab1p for their integrity and are inherited by developing daughter cells in a She2p/ She3p dependent manner. These results point to a model where roughly half the mRNA for certain translation factors are specifically directed in granules toward the tip of the developing daughter cell where protein synthesis is most heavily required, which has particular implications for filamentous forms of growth. Such a feedforward mechanism would ensure adequate provision of the translation machinery where it is to be needed most over the coming growth cycle.

show abstract

Hyphal Structure

Cited by 16 publications

References 70 publications

Yeast-to-hypha transition ofSchizosaccharomyces japonicusin response to natural stimuli

Yeast-to-hypha transition ofSchizosaccharomyces japonicusin response to natural stimuli

Subcellular structure and behaviour in fungal hyphae

Translation factor mRNA granules direct protein synthetic capacity to regions of polarized growth

Contact Info

Product

Resources

About