Hyphal wall chemistry in Apodachlya

Abstract

Hyphal walls were isolated from the Oomycete, Apodachlya sp. Microscope examination of wall preparations showed that they were clean and relatively free of cellulin granules. The principal wall constituents, accounting for more than half of wall weight, were beta-glucans with 1,3- and 1,6-glucosidic linkages. Apparently chitin was the second most abundant wall constituent (18%) and cellulose accounted for less than 10% of wall weight. Protein was a significant wall constituent (6.4%), and protein hydrolysis demonstrated nearly all common amino acids plus hydroxyproline; additionally, the unusual amino acid, hydroxylysine was tentatively identified. The lipid and ash constituents were small (1.7and 0.4%, respectively) and no particular significance was assigned to them. The possible occurrence of wall glycoproteins and the relationship between wall chemistry and systematics in Apodachlya and related genera were discussed.

Use of Molecular Cytology to Study the Structure and Biology of Phytopathogenic and Mycorrhizal Fungi

“…More recently, WGA labeling has also confirmed biochemical evidence (Lin et al, 1976;Campos-Takaki et al, 1982;Bulone et al, 1992) of the presence of a small amount of chitin in the predominantly cellulosic walls of members of the Oomycetes (Burr and Beakes, 1994;Asiegbu et al, 1996). The functional significance of this small chitin fraction remains to be determined, but knowledge of its presence is important for studies of the plant response to Oomycete pathogens.…”

A Cladistic Outline of the Eumycota

“…However, some exceptions in fungi are known. The oomycete Apodachlya is reported to have chitin as well as cellulose in its cell walls (Lin et al, 1976). In taxa above node D ( Fig.…”

Molecular architecture of the hyphal wall in the water mold, Achlya ambisexualis Raper / by Julia Barth Reiskind.

“…Apodaahlya sp. and A. bvaahynema walls differ from those of Sapromyaes in having glucosamine (Sietsma et al 1969;Lin et al 1976). X-ray diffraction studies and stains for chitin indicated the presence of both weakly crystalline cellulose I and chitin in these walls (Lin and Aronson 1970 braahynema contain phospholipids, fatty acids, and triglycerides (Sietsma et al 1969).…”

“…cellulose, 6.4% protein, 1.5% acid-soluble hexosamine, and 3.1% alkalisoluble hexosamine (Lin and Aronson 1970;Lin et al 1976). Analysis of the hyphal wall chemistry of Leptomitus laateus indicated similarity to the walls of Sapromyces and Apodaahlya, especially in regard to the linkage pattern (Aronson and Lin 1978) .…”

“…In one, the walls were cleaned chemically by a modification of Tokunaga and Bartnicki-Garcia (1971) and, in the other, they were cleaned by repeated washings with buffer and water (Lin et at. 1976 All subsequent analyses were begun with 100 mg samples of this material.…”