Hyphal wall protein 1 gene: A potential marker for the identification of different
Candida species and phylogenetic analysis

Abastabar, Mahdi; Hosseinpoor, Susan; Hedayati, Mohammad Taghi; Shokohi, Tahereh; Valadan, Reza; Mirhendi, Hossein; Mohammadi, Rasoul; Âghili, Seyed Reza; Rahimi, Nooshin; Aslani, Narges; Haghani, Iman; Gholami, Sara

doi:10.18869/acadpub.cmm.2.4.1

Cited by 14 publications

(9 citation statements)

References 43 publications

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“…The sequence difference count matrix of BT2 gene observed among the members of C. parapsilosis complex indicated that this locus may be more useful than ITS regions (84.7% versus 89.6% similarity) for the discrimination of these three closely related species (data not shown). Meanwhile, the analysis of the hyphal wall protein 1 ( HWP1 ) nucleotide sequence alignment revealed only 60% similarity between C. parapsilosis and C. orthopsilosis [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of beta-tubulin DNA sequences within Candida parapsilosis complex

Kharazi¹,

Ahmadi²,

Makimura³

et al. 2018

CMM

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Background and Purpose: Candida parapsilosis is a common cause of candidemia in children and patients with onco-hematological diseases, septic arthritis, peritonitis, vaginitis, and nail and skin infections. Regarding this, the present study was condcuted to evaluate intra- and inter-species variation within beta-tubulin DNA sequence of C. parapsilosis complex in order to establish the utilization of this gene in the identification and phylogenetic analysis of the species.Materials and Methods:A total of 23 isolates representing three different species of C. parapsilosis complex were used in this study, all of which were identifed by ITS-sequencing. For the successful amplification of beta-tubulin gene, a newly designed set of pan-Candida primers was used, followed by bilaterally sequence analysis for pairwise comparisons, determination of multiple alignments, evaluation of sequence identity levels, counting sequence difference, and construction of phylogenetic tree.Results:The multiple alignment of 623-629 bp-long nucleotide (nt) sequences reflecting the beta-tubulin gene indicated an inter-species divergence ranging within 0-68 nt in C. parapsilosis, C. orthopsilosis, and C. metapsilosis with a mean similarity of 84.7% among the species. Meanwhile, the intra-species differences of 0-20 and 0-6 nt were found between the strains of C. parapsilosis and C. orthopsilosis, respectively. The phylogenetic tree topology was characterized by a clade made up by C. parapsilosis and C. orthopsilosis, while C. metapsilosis formed a related but separate lineage.Conclusion:Our data provided the basis for further discoveries of the relationship between the species belonging to C. parapsilosis complex. Furthermore, the findigns of the prsent study revealed the efficiency of beta-tubulin DNA sequence data in the identification and taxonomy of C. parapsilosis and other pathogenic yeasts.

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Section: Discussionmentioning

confidence: 99%

Characterization of beta-tubulin DNA sequences within Candida parapsilosis complex

Kharazi¹,

Ahmadi²,

Makimura³

et al. 2018

CMM

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“…Among the targets currently explored for detection of this species in the clinical setting, hyphal wall protein 1 (HWP1), a hypha-specific cell wall protein with adhesive properties, has been suggested to be a good marker for species identification (222). The cell wall ␤-1,3-glucan content has been used to discriminate fungal cells growing in a planktonic stage from those forming biofilms.…”

Section: Cell Wall Assemblymentioning

confidence: 99%

Candida parapsilosis: from Genes to the Bedside

et al. 2019

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SUMMARY Patients with suppressed immunity are at the highest risk for hospital-acquired infections. Among these, invasive candidiasis is the most prevalent systemic fungal nosocomial infection. Over recent decades, the combined prevalence of non-albicans Candida species outranked Candida albicans infections in several geographical regions worldwide, highlighting the need to understand their pathobiology in order to develop effective treatment and to prevent future outbreaks. Candida parapsilosis is the second or third most frequently isolated Candida species from patients. Besides being highly prevalent, its biology differs markedly from that of C. albicans, which may be associated with C. parapsilosis’ increased incidence. Differences in virulence, regulatory and antifungal drug resistance mechanisms, and the patient groups at risk indicate that conclusions drawn from C. albicans pathobiology cannot be simply extrapolated to C. parapsilosis. Such species-specific characteristics may also influence their recognition and elimination by the host and the efficacy of antifungal drugs. Due to the availability of high-throughput, state-of-the-art experimental tools and molecular genetic methods adapted to C. parapsilosis, genome and transcriptome studies are now available that greatly contribute to our understanding of what makes this species a threat. In this review, we summarize 10 years of findings on C. parapsilosis pathogenesis, including the species’ genetic properties, transcriptome studies, host responses, and molecular mechanisms of virulence. Antifungal susceptibility studies and clinician perspectives are discussed. We also present regional incidence reports in order to provide an updated worldwide epidemiology summary.

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“…The identification of C. albicans species complex (i.e., C. africana, C. albicans, and C. dubliniensis) was accomplished using the partial amplification of hyphal wall proteins (HWP1) gene according to the primers designed by Romeo and Criseo (forward, 5′-GCTACCACTTCAGAATCATCATC-3′ and reverse 5′-GCACCTTCAGTCGTAGAGACG-3′) that generate the fragments of 940 and 740 bp for C. albicans and C. africana [22]. The discrimination of C. parapsilosis complex, including C. parapsilosis and C. orthopsilosis, was conducted as previously described [23].…”

Section: Molecular Identificationmentioning

confidence: 99%

Species distribution and susceptibility profiles of Candida species isolated from vulvovaginal candidiasis, emergence of C. lusitaniae

Hashemi¹,

Shokohi²,

Abastabar³

et al. 2019

CMM

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Background and Purpose: The aim of the current study was to investigate the epidemiology of vulvovaginal candidiasis (VVC) and recurrent VVC (RVVC), as well as the antifungal susceptibility patterns of Candida species isolates.Materials and Methods: A cross-sectional study was carried out on 260 women suspected of VVC from February 2017 to January 2018. In order to identify Candida species isolated from the genital tracts, the isolates were subjected to polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) using enzymes Msp I and sequencing. Moreover, antifungal susceptibility testing was performed according to the Clinical and Laboratory Standards Institute guidelines (M27-A3).Results: Out of 250 subjects, 75 (28.8%) patients were affected by VVC, out of whom 15 (20%) cases had RVVC. Among the Candida species, C. albicans was the most common species (42/95; 44.21%), followed by C. lusitaniae (18/95; 18.95%), C. parapsilosis (13/95; 13.69%), C. glabrata (8/95; 8.42%), C. kefyr (6/95; 6.31%), C. famata (5/95; 5.26%), C. africana (2/95; 2.11%), and C. orthopsilosis (1/95; 1.05%), respectively. Multiple Candida species were observed in 28% (21/75) of the patients. Nystatin showed the narrowest range of minimum inhibitory concentration (MIC) (0.25-16 μg/ml) against all Candida strains, whereas fluconazole (0.063-64 μg/ml) demonstrated the widest MIC range. In the current study, C. lusitaniae, as the second most common causative agent of VVC, was susceptible to all antifungal agents. Furthermore, 61.1% of C. lusitaniae isolates were inhibited at a concentration of ≤ 2 μg/ml, while38.9% (n=7)of them exhibited fluconazole MICs above the epidemiologic cutoff values (ECV). Candida species showed the highest overall resistance against fluconazole (61.3%), followed by itraconazole (45.2%) and caspofungin (23.7%). All of C. albicans strains were resistant to itraconazole with a MIC value of ≥ 1 μg/ml; in addition, 87.5% of them were resistant to fluconazole. Moreover, 100% and 87.5% of C. glabrata strains were resistant to caspofungin and fluconazole, respectively.Conclusion: As the findings revealed, the majority of VVC cases were caused by non-albicans Candida species which were often more resistant to antifungal agents. Candida lusitaniae generally had fluconazole MICs above the ECV. Given the propensity of C. lusitaniae to develop resistance under drug pressure, antifungals should be administered with caution. The emergence of these species justify the epidemiological surveillance surveys to watch out the distribution of yeast species.

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Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis

Cited by 14 publications

References 43 publications

Characterization of beta-tubulin DNA sequences within Candida parapsilosis complex

Characterization of beta-tubulin DNA sequences within Candida parapsilosis complex

Candida parapsilosis: from Genes to the Bedside

Species distribution and susceptibility profiles of Candida species isolated from vulvovaginal candidiasis, emergence of C. lusitaniae

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