Hyphenated analytical techniques for validation of herbal medicine

Kar, Amit; Biswas, Sayan; Chaudhary, Sushil Kumar; Banerjee, Subhadip

doi:10.1016/b978-0-323-85542-6.00038-x

Cited by 11 publications

(2 citation statements)

References 114 publications

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“…This type of analysis not only helps in the primary identification of the herbs but also gives an impression about the quality of the herbs used in phytopharmaceutical formulations based on their topography, inter/intra-species variation in plants, the content of metabolites, microbial contamination, pesticide content and post harvesting factors like the content of preservatives [5]. Phytochemical analysis and standardization of medicinal plants by hyphenated and spectroscopic techniques like HPLC-MS, HPTLC-MS, GC-MS, and HPLC-NMR are proven tools for the quality evaluation and scientific validation of medicinal plants used in traditional and folklore medicine [6]. UPLC-Quadrupole TOF-MS/MS (UPLC-QTOF-MS/MS) has become a valuable tool for the quality control of natural products due to higher sensitivity and selectivity.…”

Section: Introductionmentioning

confidence: 99%

Ultra‐performance liquid chromatography‐Quadrupole time‐of‐flight tandem mass spectrometry‐based metabolite profiling, quality evaluation, and marker analysis of Trachyspermum ammi (L.) Sprague by high‐performance thin‐layer chromatography

Gupta

Kar

Narayan

et al. 2023

J of Separation Science

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Trachyspermum ammi (L.) Sprague (Apiaceae), commonly known as “Ajwain” is distributed throughout India. Ajwain fruits contain fiber, carbohydrates, phenolic acids, flavonoids, and tannins. The fruits also yield a small amount of essential oil, with Thymol as the principal constituent. Ajwain has various pharmacological activities like anti‐leishmanial, antimicrobial, cytotoxic, antispasmodic, nematocidal, and anthelmintic. The fruits are of high therapeutic value; thus, it becomes quite essential to evaluate the quality of Trachyspermum ammi (L.) Sprague to authenticate and ensure its therapeutic and nutritional properties. The ethyl acetate fraction of Trachyspermum ammi (L.) Sprague fruits exhibited the highest total phenolic and flavonoid content values of 149.55 ± 1.19 mg rutin equivalent and 682.85 ± 3.68 mg gallic acid equivalent, respectively. Metabolite profiling of the ethyl acetate fraction using ultra‐performance liquid chromatography‐quadrupole time‐of‐flight tandem mass spectrometry analysis resulted in identifying 19 phytomolecules. A validated high‐performance thin‐layer chromatography method was developed to quantify standard phytomolecules in the ethyl acetate fraction. The highest and lowest percentages of phytomarker were found to be caffeic acid (5.51% ± 0.16% w/w) and gallic acid (1.29% ± 0.09% w/w), respectively. This validated rapid, accurate, and precise method for standardization of Trachyspermum ammi (L.) Sprague will be beneficial for its quality evaluation as well as the derived products.

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Section: Introductionmentioning

confidence: 99%

Ultra‐performance liquid chromatography‐Quadrupole time‐of‐flight tandem mass spectrometry‐based metabolite profiling, quality evaluation, and marker analysis of Trachyspermum ammi (L.) Sprague by high‐performance thin‐layer chromatography

Gupta

Kar

Narayan

et al. 2023

J of Separation Science

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“…The development of a single mobile phase for the investigation of a number of phenolic compounds would signify a beneficial and rapid solution for analyzing the occurrence of polyphenols in extracts/formulations. HPLC is a frequently used chromatographic technique that combines high resolution and easy mechanization with modest sample requirements [18]. Apart from the conventional uses, Potentilla fulgens is also consumed in several herbal preparations but very few processes are available for the determination of phenolic compounds in the Potentilla fulgens extract which may help as a quality control tool of the raw material [11].…”

Section: Introductionmentioning

confidence: 99%

Reversed phase‐high‐performance liquid chromatography simultaneous estimation of phenolic compounds in hydroalcoholic extract of Potentilla fulgens root

Chaudhary

Sharma

Sarangthem

et al. 2023

Separation Science Plus

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The roots of Potentilla fulgengs have been utilized for a long time as an astringent and tonic for treating gum and tooth diseases, microbial contagions, inflammation, diabetes mellitus, and wounds and are rich in polyphenols and flavonoids. Authentication and constant excellence are the rudimentary limitations of herbal medicines and their complexity is a key challenge for quality control. High‐performance liquid chromatography has become an important technique for the quality control of herbal medicines because of its simplicity and uniformity. It can be applied for the identification, confirmation, and quality control of herbal medicines. So, an approach using reversed‐phase high‐performance liquid chromatography was developed and validated for the simultaneous determination of phenolic compounds in the hydroalcoholic extract (methanol:water::70:30% v/v) of Potentilla fulgens roots. The results revealed that ellagic acid was found in the highest amount (8.191 ± 0.463 mg/g) followed by rutin (5.429 ± 0.062 mg/g) and caffeic acid (3.032 ± 0.453 mg/g) among the studied phenolic compounds while quercetin was not detected. The validation parameters involved selectivity, linearity, range, limits of detection and quantitation, precision, and accuracy. All calibration curves were linear (R2 > 0.99) within the range, and good precision (relative standard deviation < 2.0%) and recovery (96.129%–117.110%) were found for all standards.

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A Comprehensive Quality Evaluation for Gentiana Rigescens Franch. by Fingerprinting Combined with Chemometrics and Network Pharmacology

Li,

Zhu,

Shen

et al. 2024

Chemistry & Biodiversity

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Gentiana rigescens Franch. (G. rigescens) is a unique traditional medicinal herb from southwestern China, and its clinical mechanism for the treatment of hepatitis and the quality differences between different origins are not clear. The research aims to analyze the mechanisms for the treatment of hepatitis and differences in inter‐origin differences using analytical techniques, chemometrics, and network pharmacology. Through infrared spectroscopy, spectral images, and high‐performance liquid chromatography (HPLC) analysis, it was found that there were differences in absorbance intensity and significant differences in compound content among the samples’origin. G. rigescens iridoids and flavonoids exert therapeutic effects on hepatitis through multiple targets and multiple pathways. The above HPLC, chemometrics, and network pharmacology results revealed that gentiopicroside, and swertiamarine was the best quality marker among origins. The ResNet model could be utilized as an effective tool for tracing G. rigescens’s origins. The PLSR model had excellent predictive performance in determining the content of gentiopicroside and swertiamarine, and could quickly, accurately, and effectively predict these two compounds. The research investigates the differences in G. rigescens origins from multiple perspectives, establishes image recognition models and prediction models, and provides new methods and theoretical basis for quality control of G. rigescens.

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Hyphenated analytical techniques for validation of herbal medicine

Cited by 11 publications

References 114 publications

Ultra‐performance liquid chromatography‐Quadrupole time‐of‐flight tandem mass spectrometry‐based metabolite profiling, quality evaluation, and marker analysis of Trachyspermum ammi (L.) Sprague by high‐performance thin‐layer chromatography

Ultra‐performance liquid chromatography‐Quadrupole time‐of‐flight tandem mass spectrometry‐based metabolite profiling, quality evaluation, and marker analysis of Trachyspermum ammi (L.) Sprague by high‐performance thin‐layer chromatography

Reversed phase‐high‐performance liquid chromatography simultaneous estimation of phenolic compounds in hydroalcoholic extract of Potentilla fulgens root

A Comprehensive Quality Evaluation for Gentiana Rigescens Franch. by Fingerprinting Combined with Chemometrics and Network Pharmacology

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