Hypo-osmotic test in cat spermatozoa

Abstract

The hypo-osmotic (HOS) test has been used in other species as an indicator of the fertilising capacity of spermatozoa. The aims of this study were to assess the response of domestic cat spermatozoa to the hypo-osmotic test, to determine the type of solution, concentration and time of incubation needed to obtain a maximum percentage of swelling, to correlate the selected combination with the percentages of progressive motility and to evaluate whether dilution of the ejaculate alters the results. Incubation for 30 and 45 min in solutions of fructose and of citrate of 50 and 100 mOsmol kg⁻¹ was evaluated. The highest percentage of swelling was obtained using the 50 mOsmol kg⁻¹ solution, and no significant differences were observed between the times of exposure to the solutions. A positive correlation was observed between the percentage of individual progressive motility and the percentage of sperm swelling in a 50 mOsmol kg⁻¹ fructose solution, with no significant differences being observed between raw and diluted semen samples. The results of this study suggest that the HOS test could be useful for evaluating membrane function in domestic cat spermatozoa, both in raw semen and in samples diluted in the EZ Mixin® commercial extender, and thus could be incorporated into routine semen evaluation protocols.

Cryopreservation and evaluation of Akkaraman ram semen with 7-dehydrocholesterol

“…Because these spermatological parameters (motility, HOS test, viability) are dependent on sperm plasma membrane integrity, similar results are expected and their results confirm our results. Although some authors (9,20) found correlation between morphological defect and HOS test, we did not observe any interaction between these parameters. So, these parameters should be evaluated seperately.…”

“…Cholesterol is a necessary sterol for cell proliferation and viability. In addition, cholesterol enables cell interactions, affects membrane phase transition, ensures suitable environments for membrane-associated proteins, facilitates morphological membrane characteristics, serves as a membrane antioxidant and decreases membrane permeability by stabilizing the membrane (9).…”

Mitigation of sperm tail abnormalities using demembranation approach in the clouded leopard (Neofelis nebulosa)

“…The hypo‐osmotic (HOS) test used in this study was modified from Comercio et al, (). Briefly, aliquots (5 μl) of each sample (raw ejaculate, simple washed and SLC‐selected) were treated with 50 μl of the hypo‐osmotic (HOS) solution (60 mOsm/kg of fructose solution in sodium citrate) and incubated at 37°C for 45 min.…”

Review on the screening of semen by hypo-osmotic swelling test

“…On the basis of these strong correlations between HOST and semen evaluation parameters, HOST was recommended as the semen screening in hospitals for men (Oosterhuis et al ., ) and replacement of routine semen evaluation parameters such as motility, morphology and livability in men (Jayendran et al ., ), boar (Samardžija et al ., ), ram (Bohlooli et al ., ), horse (Neild et al ., ), dog (Rodriguez‐Gil & Rigau, ), goat (Fonseca et al ., ), Spanish free‐range poultry (Santiago‐Moreno et al ., ,b) and cat (Comercio et al ., ). The application of HOST may be an important tool in the routine identification and selection of viable and DNA‐intact individual spermatozoa for ICSI after further research to demonstrate its efficacy and safety (James et al ., ).…”