Hypochlorous acid triggered fluorescent probes for<i>in situ</i>imaging of a psoriasis model

Wei, Peng; Yu, Xiao‐Qi; Liu, Lingyan; Zhou, Xiaojun; Yi, Tao

doi:10.1039/d2tb00765g

Cited by 12 publications

(4 citation statements)

References 63 publications

(76 reference statements)

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“…Furthermore, after addition of HOCl, an absorption peak at around 665 nm and an emission peak at around 688 nm occurred, which corresponded with the absorption peak (665 nm) and emission peak (692 nm) of methylene blue, demonstrating that methylene blue is released when the probes interact with HOCl. We hypothesize that the mechanism of probes reacting to HOCl is depicted in Figure b based on relevant literature studies. , HOCl nucleophilically attacks the electron-deficient carbon atom in the amide carbon group and then further oxidizes it to separate the amide moiety from the methylene blue moiety. This process releases the fluorophore methylene blue and the unstable carbamic acid structure, which is then rapidly hydrolyzed in aqueous solution to form the amino group.…”

Section: Results and Discussionmentioning

confidence: 99%

“…We hypothesize that the mechanism of probes reacting to HOCl is depicted in Figure 2b based on relevant literature studies. 59,60 HOCl nucleophilically attacks the electron-deficient carbon atom in the amide carbon group and then further oxidizes it to separate the amide moiety from the methylene blue moiety. This process releases the fluorophore methylene blue and the unstable carbamic acid structure, which is then rapidly hydrolyzed in aqueous solution to form the amino group.…”

Section: S20 S29 and S31) The Above Results Indicate That The Probesmentioning

confidence: 99%

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Dual-Channel/Localization Single-Molecule Fluorescence Probe for Monitoring ATP and HOCl in Early Diagnosis and Therapy of Rheumatoid Arthritis

Qu,

Tian,

Yang

et al. 2024

Anal. Chem.

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Rheumatoid arthritis (RA), a common chronic inflammatory illness, is still incurable, reducing the sufferers' quality of life significantly. Adenosine 5′-triphosphate (ATP) and hypochlorous acid (HOCl) are key indicators in RA, but their precise mechanisms in RA pathophysiology are unknown. As a result, in order to detect ATP and HOCl simultaneously, we created two new dualchannel/localization single-molecule fluorescence probes, RhTNMB and RhFNMB. Furthermore, RhFNMB outperformed RhTNMB in terms of detection performance. ATP and HOCl produce independent fluorescence responses in the light red channel (λ ex = 520 nm, λ em = 586 nm) and deep red channel (λ ex = 620 nm, λ em = 688 nm), respectively, without spectral crosstalk. It should be noted that the probe RhFNMB successfully imaged ATP in mitochondria and HOCl in cells. Surprisingly, the probe RhFNMB demonstrated remarkable detection ability in the diagnosis and treatment of Pseudomonas aeruginosa-induced abdominal inflammation in mice. We continued to apply the probe RhFNMB to track ATP and HOCl in RA and discovered that ATP and HOCl concentrations were considerably greater in RA joints than in normal joints. We also confirmed the therapeutic effect of methotrexate on RA. This study is the first to achieve dual-channel imaging of ATP and HOCl, which is of great value for the early diagnosis and therapy of RA.

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Section: Results and Discussionmentioning

confidence: 99%

Section: S20 S29 and S31) The Above Results Indicate That The Probesmentioning

confidence: 99%

Dual-Channel/Localization Single-Molecule Fluorescence Probe for Monitoring ATP and HOCl in Early Diagnosis and Therapy of Rheumatoid Arthritis

Qu,

Tian,

Yang

et al. 2024

Anal. Chem.

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“…In recent years, several other articles (probes 15 and 17) have reported the use of such phenazine derivatives similar to probe 13 in the detection of arthritis. [164][165][166] Due to the sensitivity of phenazine derivatives in detecting HClO, these phenazine compounds could be further developed for in-depth arthritis studies.…”

Section: Fl Imaging Of Arthritismentioning

confidence: 99%

Emerging advances in fluorescence imaging and phototherapy of arthritis

Wang,

Dai,

Shao

et al. 2023

Interdisciplinary Materials

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Arthritis is a chronic disease whose etiology is difficult to pinpoint, and the difficulty of arthritis detection and subsequent treatment causes enormous distress to patients. In recent years, thanks to advances in medicine and detection, a variety of treatment modalities for arthritis have emerged. The combination of emerging detection technologies with different anti‐inflammatory medications and even advances in surgical techniques have all played a positive role in the treatment of arthritis. In the present work, we have collected relevant literature on fluorescence (FL) imaging and phototherapy of arthritis in recent years, intending to reveal the advantages and potential application value of FL imaging and phototherapy for researchers. Meanwhile, due to the shortcomings of FL imaging and phototherapy in the diagnosis and treatment of arthritis, we advocate overcoming these difficulties in future research.

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“…In our previous studies, we have successfully developed a series of HOCl-specific fluorescent probes using methylene blue (MB) structure and utilized them to investigate the presence of HOCl in diverse conditions such as the arthritis 36 , tumor microenvironment 37 - 39 , psoriasis 40 , and other diseases 41 - 43 . Herein, we designed and synthesized a fluorescent probe DHU-CBA1 by attachment of IBP (ibuprofen, a nonsteroidal anti-inflammatory drug) and MB.…”

Section: Introductionmentioning

confidence: 99%

A smart hypochlorous acid fluorescent probe enabling Ibuprofen-release for osteoarthritis theranostics

Lu,

Wei,

Peng

et al. 2024

Theranostics

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Background: Osteoarthritis (OA) standing as the most prevalent form of arthritis, closely associates with heightened levels of reactive oxygen species, particularly hypochlorous acid (HOCl). Although there are numerous probes available for detecting HOCl in the OA region, probes with dual functions of diagnostic and therapeutic capabilities are still significantly lacking. While this type of probe can reduce the time gap between diagnosis and treatment, which is clinically needed. Methods: We developed a fluorescent probe (DHU-CBA1) toward HOCl with theranostics functions through the release of methylene blue (MB) and ibuprofen (IBP) in this work. DHU-CBA1 can detect HOCl with high specificity and sensitivity, releasing MB and IBP with an impressive efficiency of ≥ 95% in vitro . Results: DHU-CBA1 exhibits good biosafety, enabling in vivo imaging of endogenous HOCl, along with reducing arthritis scores, improving synovitis and cartilage damage, and maintaining catabolic balance while alleviating senescence in cartilage. Conclusions: This study proposes a novel approach to enhance osteoarthritis therapy by releasing IBP via a smart HOCl-enabled fluorescent probe.

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Hypochlorous acid triggered fluorescent probes forin situimaging of a psoriasis model

Abstract: Psoriasis is a common skin disease with complex pathogenesis that lacks of diagnostic methods. Typically, psoriasis is an inflammation-related disease accompanied by high expression of reactive oxygen species (ROS) in...

Cited by 12 publications

References 63 publications

Dual-Channel/Localization Single-Molecule Fluorescence Probe for Monitoring ATP and HOCl in Early Diagnosis and Therapy of Rheumatoid Arthritis

Dual-Channel/Localization Single-Molecule Fluorescence Probe for Monitoring ATP and HOCl in Early Diagnosis and Therapy of Rheumatoid Arthritis

Emerging advances in fluorescence imaging and phototherapy of arthritis

A smart hypochlorous acid fluorescent probe enabling Ibuprofen-release for osteoarthritis theranostics

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