Hypoglycemic Activity of Self-Assembled Gellan Gum-Soybean Isolate Composite Hydrogel-Embedded Active Substance-Saponin

Abstract

In order to avoid hemolysis caused by direct dietary of kidney tea saponin, complex gels based on gellan gum (GG) and soybean isolate protein (SPI) loaded with saponin were created in the present study by using a self-assembly technique. Studies were conducted on the rheological characteristics, encapsulation effectiveness, molecular structure, microstructure, and hypoglycemic activity of GG/SPI-saponin gels. Increasing the concentration of SPI helped to enhance the strength and energy storage modulus (G′) of the gels, and the incorporation of high acylated saponin allowed the whole gel to undergo sol–gel interconversion. The encapsulation efficiency showed that GG/SPI-saponin was 84.52 ± 0.78% for saponin. Microstructural analysis results suggested that GG and SPI were bound by hydrogen bonds. The in vitro digestion results also indicated that saponin could be well retained in the stomach and subsequently released slowly in the intestine. In addition, the in vitro hypoglycemic activity results showed that the IC50 of encapsulated saponin against α-glucosidase and α-amylase were at 2.4790 mg/mL and 1.4317 mg/mL, respectively, and may be used to replace acarbose for hypoglycemia.

Synthesis and Characterization of PolyHIPE-Gellan Gum Material with Tunable Macroporosity for Tissue Engineering Applications

“…The high remaining weight ratio of pure GG at 800 °C is attributed to the formation of char residue, and this has been claimed as a common behavior of polysaccharide-based materials under high temperature conditions. Similar results have been obtained by Wu et al and Lavikainen et al…”

“…The high remaining weight ratio of pure GG at 800 °C is attributed to the formation of char residue, and this has been claimed as a common behavior of polysaccharide-based materials under high temperature conditions. Similar results have been obtained by Wu et al 37 and Lavikainen et al 38 The results indicate that both pure polyHIPE and polyHIPE mixed with GG (0.1 and 0.5%) exhibit good thermal stability compared to pure GG. Thermal stability of biomaterials is crucial, especially during the material sterilization process via dry heat treatment, a typical dry heat sterilization is reported around 160 °C for a duration of 2 h. 39 This method is considered more effective than steam autoclave treatment prior to application in cell culture studies or implantation as it helps prevent infections caused by scaffold contamination.…”

Synergism of a Novel Bio-Based Surfactant Derived from Pisum sativum and Formation Brine for Chemical Enhanced Oil Recovery in Carbonate Oil Reservoirs

“…The weight loss at temperatures of 175 to 600 °C is generally due to the breakdown of glycosidic bonds and intermolecular hydrogen bonds. The TGA analysis result for the PS surfactant is on par with other green surfactants reported in the scientific literature [27][28][29]31,32]. The PS green surfactant is analyzed by 1 H NMR, as illustrated in Figure 10.…”

“…ported in the scientific literature [27][28][29]31,32]. The PS green surfactant is analyzed by 1 H NMR, as illustrated in Figure 10.…”

Injectable Tissue Adhesive Microgel Composite Containing Antifibrotic Drug for Vocal Fold Scarring

“…FTIR analysis of the samples revealed the characteristic bands of each of the components in the gel in the range of 400−4000 cm −1 (Figure 2D). The peaks corresponding to sodium alginate (uronic acid at 939 cm −1 and CH 2 stretching at 2928 cm −1 ), 17 gelatin (1660 cm −1 for C�O stretching, 1554 cm −1 for N−H deformation, and 2850 cm −1 for C−H stretching), 18,19 and GG (1418 cm −1 for CH stretching of methyl groups, 20 1026 cm −1 for N−H stretching, and 2927 cm −1 for CH 2 stretching). 21 Similar peaks were observed in both AG-GG and AG B GG, signifying that the chemical compositions were not changed after the blending of gellan gum with alginate and gelatin.…”