Hypoglycemic and antioxidant effects of Hibiscus rosa-sinensis L. leaves extract on liver and kidney damage in streptozotocin induced diabetic rats

Abstract

Hibiscus is commonly used traditionally for the treatment of some diseases such as hypertension and as antidiabetic herbal medicine. The objective of the present study is to investigate the effect of the oral administration of aqueous methanolic extract of Hibiscus rosa-sinensis leaves (400 mg/Kg) on streptozotocin (STZ) induced diabetic rats and alteration in liver and kidney functions. The treatment of diabetic rats with hibiscus leaves extract reduced levels of plasma glucose, cholesterol, aspartate aminotransferase (AST), alanine aminotransferase (ALT), uric acid and creatinine and hepatic malondialdehyde that was elevated in diabetic rats. Moreover, the Hibiscus leaves extract mitigates the decrease in hepatic superoxide dismutase and plasma protein levels due to STZ injection. The treatment of rats with STZ only results in some pathological effects in liver and kidneys as degeneration in most of hepatocyte and glomeruli. The extract of H. rosa-sinensis leaves reduced the pathological changes. The treatment of diabetic rats with Hibiscus extract was shown to have hepatic and renal protective effects in diabetic rats induced experimentally. Here, two compounds, that is, orientin (Luteolin-8-C-glucoside) and verbascoside (phenylpropanoids glycoside) were isolated from H. rosasinsensis. The two compounds were identified by spectral analysis (UV, 1 H and 13 C-NMR). These results clearly indicate that aqueous leaves extract of H. rosa-sinensis possess antidiabetic and hypolipidemic effects in diabetic rats which may be due to antioxidant properties of the hibiscus extract.

Neuroprotective Potential of Verbascoside Isolated from Acanthus mollis L. Leaves through Its Enzymatic Inhibition and Free Radical Scavenging Ability

“…The ethyl acetate extract was separated using silica gel column chromatography and provided compound 1 as white needles after recrystallization with MeOH. This compound was characterized by spectroscopic analyses, including 1 H NMR, 13 C NMR, 2D NMR ( 1 H-1 H COSY, HMBC), FAB-MS, and UV, and was identified as verbascoside through comparison of its spectral features with the data available in the literature [35].…”

Antidiabetic activity‐guided isolation of gallic and protocatechuic acids fromHibiscus sabdariffacalyxes

“…The serum and liver/pancreas homogenates of sacrificed animals were prepared according to the procedure outlined by Ajiboye, Yakubu, and Oladiji (2014). The aqueous extract of H. sabdariffa was administered in 100, 200, and 400 mg/kg BW (Zaki et al, 2017).…”

Synthesis of Ag0nanoparticles From Extract and Ethanolic and Aqueous Fractions of Leaves and Petals of Hibiscus Rosa-Sinensis L.

“…Analysis by UHPLC-ESI-Q-Orbitrap MS-MS of the EtOH extract of the flower petals of Hibiscus rosa-sinensis L provided the molecular weight of the structures present. The flavonoids pelargonidin [31], [32], peonidin [33], kaempferol [34], [35], orientin [36], astilbin [37], [38], gossypin [39], were identified in the ethanolic extract of the petals of H. rosa-sinensis L (Table 2). Compound 3 showed a fragment of m/z 285, which corresponds to the mass of kaempferol as aglycone, while the difference (308) corresponds to the loss of a rutinoside (glucose-nose disaccharide), which indicates the presence of the kaempferol rutinoid, which has already been notified [35].…”