Hypoglycemic and hypolipidemic effects of tectorigenin and kaikasaponin III in the streptozotocin-induced diabetic rat and their antioxidant activityin vitro

Lee, Kyung Tae; Sohn, Il Cheol; Kim, Dong Hyun; Choi, Jong Won; Kwon, Sang Hyuk; Park, Hee Juhn

doi:10.1007/bf02976573

Cited by 100 publications

(75 citation statements)

References 19 publications

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“…Flavonoids also lead to regeneration of pancreatic β-cells, reduce necrosis and degeneration of pancreatic β-cells and thus may be effective in treating hyperglycemia by preventing diabetic complications [26]. Flavonoids are also known to decrease triglyceride level [27]. Phenolic compounds are also known to decrease blood glucose in STZ-induced diabetic rats and increase in insulin secretion, which may be due to regeneration of β-cells brings by reduction in oxidative stress [28].…”

Section: Discussionmentioning

confidence: 99%

Anti-Hyperglycemic Effect of Different Fractions of Annona Reticulata Leaf

Rout

Kar

Maharana

2016

Asian J Pharm Clin Res

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Context: Several species of the genus Annona were reported to have hypoglycemic properties and this makes Annona reticulata Linn. (Annonaceae) an interesting plant for investigating its anti-hyperglycemic potential.Objective: Different fractions prepared from hydro-alcoholic extract of A. reticulata leave were investigated for their blood glucose lowering effect on Streptozotocin (STZ) induced hyperglycemic rats.Methods: Ethyl acetate, methanol, and residual fractions (at dose level of 100 mg/kg by oral route) prepared from the hydro-alcoholic extract of A. reticulata leave were administered for 14 consecutive days to STZ induced hyperglycemic rats for evaluation of their anti-hyperglycemic potential. Anti-hyperglycemic potential was assessed by observation of a decrease in fasting blood glucose level. Results:The studies revealed that ethyl acetate fraction decreased the blood glucose level of hyperglycemic rats from 447.67 to 234.17 mg/dL and is significant (p<0.001) when compared with diabetic control group. The residual fraction and methanolic fraction decreased blood glucose level from 417.83 to 402.50 mg/dL and 432.33 to 371.67 mg/dL respectively but not significant when compared with the diabetic control group. Standard drug metformin (dose 300 mg/kg) reduced the blood glucose level from 447.33 to 219.50 mg/dL. Discussion: Ethyl acetate fraction at tested dose level was capable not only to control the elevated blood glucose level but also able to attenuate certain secondary parameters associated with STZ induced hyperglycemia. Conclusion:This study suggested that the ethyl acetate fraction prepared from hydro-alcoholic extract of A. reticulata leave exhibit potential antihyperglycemic property in the tested experimental models and should be investigated further.

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Section: Discussionmentioning

confidence: 99%

Anti-Hyperglycemic Effect of Different Fractions of Annona Reticulata Leaf

Rout

Kar

Maharana

2016

Asian J Pharm Clin Res

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“…Intra-peritoneal administration of tectorigenin significantly reduced the blood glucose, total cholesterol, LDL-cholesterol, VLDL-cholesterol and triglyceride levels in the streptozotocin-induced diabetic rats, thus showing potent hypoglycaemic and hypolipidaemic effects in vivo (Lee et al, 2000).…”

Section: Hypoglycaemic and Hypolipidaemic Effectsmentioning

confidence: 91%

“…Most of the compounds reported belong to the isoflavonoid family, including tectorigenin (1), tectorigenin-7-O-β-glucosyl (1→6) glucoside (2), tectoridin (3), irigenin (4), iridin (5), irisflorentin (6), dichotomitin (7), 3',5'-dimethoxy irisolone-4-O-β-D-glucoside (8), nonirisflorentin (9), iristectorigenin A (10), iristectorigenin B (11), 5,6,7,3'-tetrahydroxy-4'-methoxyisoflavone (12), 6'-O-vanilo-riridin (13), iristectrigenin (17), iristectrigenin (17), iristectrigenin A-7-glucoside (18), 8-hydroxytectrigenin (19), 8-hydroxyiristectrigenin A (20), 8-hydroxyirigenin (21), tectrigenin-4'-glucoside (22), irilin D (23), isotectrigenin (24), astragalin (25), 5,7,4'-dihydroxy-6,3',5'-trimethoxyisoflavone (26), genistein (27), dimethyltectorigenin (28), isoirigenin (29), isoirigenin-7-O-β-D-glucoside (30), irisolon (31), 3'-hydroxytectoridin (32), and tectoruside (33). Compounds that belong to the flavonoid family (Jin et al, 2008a) include 5,4'dihydroxy-6,7-methylenedioxy-3'methoxyflavone (38), iristectogenin (39), hispidulin (40) and acetovanillone (41). Flavonol compounds include isorhamnetin (42), rhamnazin (43) and rhamnocitrin (44).…”

Section: Isoflavones and Flavonoidsmentioning

confidence: 99%

“…Using a biochemiluminescence technique, Qin et al (2003b) found that the Isoflavones isolated from Belamcanda chinensis, including irigenin, tectorigenin, tectoridin and 5, 6, 7, 4'-tetrahydroxy-8-methoxyisoflavone, could scavenge superoxide radicals, hydroxyl radicals and hydrogen peroxide in the system, with tectorigenin being the strongest oxygen free radical scavenger of the four. In vitro studies showed that tectorigenin had antioxidant effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, xanthine-xanthine oxidase superoxide anion radical, and lipid peroxidation in rat microsomes induced by enzymatic and non-enzymatic methods (Lee et al, 2000). The isoflavonoid fractions of Belamcanda chinensis have the capability to scavenge free radicals, to reduce transition-metal ions and to protect polyunsaturated fatty acids from peroxidation (Dorota et al, 2010).…”

Section: Scavenging Free Radicals and Anti-oxidative Activitymentioning

confidence: 99%

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<i>Belamcanda chinensis</i> (l.) Dc: Ethnopharmacology, phytochemistryand pharmacology of an important traditional Chinese medicine

Xin¹,

Zheng²,

Cheng³

et al. 2015

Afr. J. Trad. Compl. Alt. Med.

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“…[37][38][39] It demonstrated the screening utilized by CU-LC-MS was effective and conclusive. This method exhibited acceptable screening efficiency and identification capability.…”

mentioning

confidence: 93%

Analysis of Xanthine Oxidase Inhibitors fromPuerariae flosUsing Centrifugal Ultrafiltration Coupled with HPLC-MS

Liu¹,

Xiao²,

Ma³

et al. 2016

Journal of the Brazilian Chemical Society

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In this study, centrifugal ultrafiltration coupled with high performance liquid chromatographymass spectrometry was utilized to screen and identify xanthine oxidase inhibitors from Puerariae flos extract. The experimental conditions of centrifugal ultrafiltration including xanthine oxidase concentration, incubation time, pH and temperature were optimized. At the optimum condition (xanthine oxidase concentration: 30.0 μg mL -1 , incubation time: 20 min, pH 7.0 and temperature: 25 °C), four compounds were successfully screened from P. flos extract and identified as tectoridin, daidzin, ononin and biochanin A. The yields of tectoridin, daidzin, ononin and biochanin A were 0.231, 0.117, 0.303 and 0.089 g from 50.0 g crude P. flos samples. The inhibitory activities of these compounds were verified by xanthine oxidase inhibition assays. The experimental half maximal inhibitory concentration (IC 50 ) values of tectoridin, daidzin, ononin and biochanin A were 88.5, 85.1, 88.8 and 87.0 μmol L -1 , and the binding degree of them were 5. 70, 8.28, 6.31 and 37.83% at the optimum condition, respectively. The proposed method provided a rapid and effective way to screen and analyze active compounds from natural products. Keywords: HPLC-MS, inhibitor, Puerariae flos, ultrafiltration, xanthine oxidase IntroductionNatural products have been used as the most consistently successful resources of new drug discovery for a long time because of their great diversity of the chemical structures and better drug-like properties compared to the synthetic compounds.1 However, natural products resources like plant extracts were very complex and usually contained various kinds of components. Separation and purification of natural compounds were time-consuming and laborious processes.2 Hence, simple and effective methods aiming at directly screening natural product extracts would be greatly helpful for drug discovery. Centrifugal ultrafiltration (CU) utilized centrifugal force and a semi-permeable membrane to retain suspended solids and high molecular weight solutes, while liquid and low molecular weight solutes were allowed to pass through depending on the nominal molecular weight cut-off of the membrane. 4 Based on these features, active compounds could be retained by membrane together with enzyme due to the binding with enzyme. Thus, CU became a useful technique for screening active compounds bound to biomacromolecules such as bovine serum albumin, 5 α-glucosidase, 6 quinone reductase-2, 7 deoxyribonucleic acid (DNA) and liposomes. 8,9 High performance liquid chromatography-mass spectrometry (HPLC-MS or LC-MS) has been widely applied for the simultaneous separation and identification of active compounds in complex mixtures. 10The combination of CU and LC-MS (CU-LC-MS) became a powerful tool in analyzing active compounds due to its simple operation, high speed and low sample consumption. Compared with immobilized enzyme screening assay, complex synthesis procedures could be avoided in CU-LC-MS as well.11 Moreover, the efficient screeni...

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Hypoglycemic and hypolipidemic effects of tectorigenin and kaikasaponin III in the streptozotocin-induced diabetic rat and their antioxidant activityin vitro

Cited by 100 publications

References 19 publications

Anti-Hyperglycemic Effect of Different Fractions of Annona Reticulata Leaf

Anti-Hyperglycemic Effect of Different Fractions of Annona Reticulata Leaf

<i>Belamcanda chinensis</i> (l.) Dc: Ethnopharmacology, phytochemistryand pharmacology of an important traditional Chinese medicine

Analysis of Xanthine Oxidase Inhibitors fromPuerariae flosUsing Centrifugal Ultrafiltration Coupled with HPLC-MS

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