Hypoglycemic Effect and Antioxidant Potential of Kaempferol-3,7-O-(α)-dirhamnoside from Bauhinia forficata Leaves

Abstract

In vivo and in vitro treatments were carried out to investigate the effects of kaempferol-3,7-O-(alpha)-dirhamnoside (kaempferitrin), a major flavonoid compound of the n-butanol fraction from Bauhiniaforficata leaves, on serum glucose levels, as well as its antioxidant potential. Oral administration of kaempferitrin led to a significant hypoglycemic effect in normal and in alloxan-induced diabetic rats. In normal rats, blood glucose lowering was observed only with the higher dose of kaempferitrin (200 mg/kg) at 1 h after treatment. However, the hypoglycemic effect of kaempferitrin in diabetic rats was evident at all doses tested (50, 100, and 200 mg/kg), and this profile was maintained throughout the period studied for both higher doses. Additionally, in glucose-fed hyperglycemic normal rats, the kaempferitrin failed to decrease blood glucose levels. In vitro antioxidant properties or action against reactive oxygen species of this compound was also evaluated. The compound showed high reactivity with 1,1-diphenyl-2-picryl hydrazyl (DPPH), IC(50) of 84.0 +/- 7.8 microM, inhibited myeloperoxidase activity with K(0.5) = 86 +/- 9.9 microM, and decreased lipid peroxidation, induced by ascorbyl radical either in microsomes or in asolectin and phosphatidylcholine liposomes, with IC(50)'s of 320 +/- 14.1, 223 +/- 8.3, and 112 +/- 8.8 microM, respectively.

Naphthalenemethyl ester derivative of dihydroxyhydrocinnamic acid, a component of cinnamon, increases glucose disposal by enhancing translocation of glucose transporter 4

“…Many bioactive compounds with anti-diabetic effects have been found in medicinal plants [30][31][32] including cinnamon [9][10][11][12][13]. In this study, a series of synthesised derivatives of DHH, which was purified from cinnamon, were tested for glucose transport activity in vitro.…”

Bioactivity and potential therapeutic benefits of some medicinal plants from the Caatinga (semi-arid) vegetation of Northeast Brazil: a review of the literature

“…Thus, besides its antidiabetic action, C. sicyoides has been furthermore evaluated for its anticonvulsant property in Brazil, where it is also used against epilepsy (Elisabetsky et al, 1988). In fact, in the leaves of this species has been identified the presence of α-tocopherol, a compound proved to protect against pentylenotetrazol pharmacological potency (Havsteen, 1983) and, among some of the constituents of B. forficata, most studies have examined the effects of natural flavonoids in physiological and pathological conditions of glucose metabolism, as well as in lipid peroxidation (Damasceno et al, 2004;De Sousa et al, 2004). Thus, such studies evidenced that B. forficata has significant antioxidant activity, beneficial in the prevention of diabetes complications associated with oxidative stress (Khalil et al, 2008).…”

“…Some reports have attributed the effects of the B. forficata leaf extracts to the main flavonoid derivative (kaempferitrin) (Jorge et al, 2004;De Sousa et al, 2004). In fact, kaempferitrin was found to have an acute lowering effect on blood glucose in diabetic rats and to stimulate the glucose uptake percentile, as efficiently as insulin in muscle from normal rats (Jorge et al, 2004).…”

Perfil de flavonoides e avaliação do potencial antioxidante e citotóxico de Bauhinia purpurea (Fabaceae) da região amazônica

“…[10][11][12] De um modo geral, as propriedades terapêuticas das espécies, especialmente as atividades antioxidante e hipoglicemiante, têm sido correlacionadas à presença de compostos fenólicos, em especial de flavonoides glicosilados, como a kaempferitrina, vinculada à ação hipoglicemiante da espécie B. forficata. [13][14][15] O estudo da espécie B. purpurea frente à atividade antioxidante vem ganhando destaque no estudo do gênero, já que seus extratos têm exibido potencial intermediário ou equivalente aos padrões geralmente utilizados nos testes para esta atividade. As substâncias fenólicas, atribuídas como as principais responsáveis por essas atividades, tem sido investigadas por meios cromatográficos e espectrométricos, na busca para estabelecer um perfil cromatográfico dentro da espécie.…”