Hypoglycemic Effect of Fermented Soybean Culture Mixed with Mulberry Leaves on Neonatal Streptozotocin-Induced Diabetic Rats

황교열,; 김영훈,; 조용석,; 박영식,; 이재연,; 강경돈,; 김근,; 주동관,; 안덕균,; 성수일,

doi:10.3746/jkfn.2008.37.4.452

Cited by 15 publications

(4 citation statements)

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“…The DNJ content and AGI activity of Bacillus subtilis MORI-fermented soybean extract (BTD-1) were approximately 5-fold higher than those of mulberry leaves [26,27]. Our previous study showed the glucose-lowering effect of BTD-1 in db/db mice.…”

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confidence: 99%

“…Recently, a paper has reported that a new species isolated from Bacillus subtilis MORI produces both AGI and DNJ in high quantities, and is present in traditionally fermented Korean soybean [ 25 ]. The DNJ content and AGI activity of Bacillus subtilis MORI-fermented soybean extract (BTD-1) were approximately 5-fold higher than those of mulberry leaves [ 26 , 27 ]. Our previous study showed the glucose-lowering effect of BTD-1 in db/db mice.…”

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confidence: 99%

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Anti-hyperlipidemic effect of soybean extract fermented byBacillus subtilisMORI indb/dbmice

et al. 2012

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The purpose of this study was to investigate the anti-hyperlipidemic effect of soy bean extract solution fermented by Bacillus subtilis MORI (BTD-1E) in obese db/db mice. Eight-week-old male db/db mice were administered 33.3 mg/kg BTD-1E solution orally once a day for four weeks. The BTD-1E group showed significantly lower body weight compared with the db control group (P<0.05). The BTD-1E group showed significantly lower serum total cholesterol and LDL cholesterol levels compared with the db control group, respectively (P<0.05, P<0.01). The BTD-1E group showed significantly decreased liver weight relative to final body weight compared with the db control group (P<0.01). After four weeks of BTD-1E administration, lipid droplets in the liver were apparently decreased in the BTD-1E group compared to the db control group. In summary, our results suggest that BTD-1E has an anti-hyperlipidemic effect in the obese mouse model.

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Anti-hyperlipidemic effect of soybean extract fermented byBacillus subtilisMORI indb/dbmice

et al. 2012

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“…These enzymes are expressed on the surface of the epithelial cells of the brush border in the small intestine. Thus, -glucosidase inhibitors such as DNJ can be used therapeutically in the oral treatment of the non-insulin-dependent (type II) diabetes mellitus (Yoshikuni et al, 1988;Asano et al, 1994Asano et al, , 2000Watson et al, 2001;Jang and Rhee, 2004;Cho et al, 2008;Hwang et al, 2008;Kong et al, 2008;Schedel, 2008). In addition, it has been suggested that DNJ can be developed as a more efficient antidiabetic by chemical derivatization to improve retention in the small intestine (Asano et al, 2000;Watson et al, 2001;Schedel, 2008).…”

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confidence: 94%

Identification of the genes involved in 1-deoxynojirimycin synthesis in Bacillus subtilis MORI 3K-85

Kang¹,

Cho

Song

et al. 2011

J Microbiol.

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1-Deoxynojirimycin (DNJ), a D-glucose analogue with a nitrogen atom substituting for the ring oxygen, is a strong inhibitor of intestinal α-glucosidase. DNJ has several promising biological activities, including its antidiabetic, antitumor, and antiviral activities. Nevertheless, only limited amounts of DNJ are available because it can only be extracted from some higher plants, including the mulberry tree, or purified from the culture broth of several types of soil bacteria, such as Streptomyces sp. and Bacillus sp. In our previous study, a DNJ-producing bacterium, Bacillus subtilis MORI, was isolated from the traditional Korean fermented food Chungkookjang. In the present study, we report the identification of the DNJ biosynthetic genes in B. subtilis MORI 3K-85 strain, a DNJ-overproducing derivate of the B. subtilis MORI strain generated by γ-irradiation, xhe genomic DNA library of B. subtilis MORI 3K-85 was constructed in Escherichia coli, and clones showing α-glucosidase inhibition activity were selected. After DNA sequencing and a series of subcloning, we were able to identify a putative Operon which consists of gabT1, yktc1, and gutB1 genes predicted to encode putative transaminase, phosphatase, and oxidoreductase, respectively. When a recombinant plasmid containing this Operon sequence was transformed into an E. coli strain, the resulting transformant was able to produce DNJ into the culture medium. Our results indicate that the gabT1, yktc1, and gutB1 genes are involved in the DNJ biosynthetic pathway in B. subtilis MORI, suggesting the possibility of employing these genes to establish a large-scale microbial DNJ overproduction system through genetic engineering and process optimization.

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“…양잠소재 중 기능성 식품 소재로 가장 많이 활용되어 온 뽕잎(Morus alba L., mulberry leaf)에는 flavones, 식물성 스 테롤, triterpenes 등의 생리활성 물질이 다량 함유되어 있 고 9) , 특히 γ-aminobutyric acid (GABA)는 녹차 잎에 비해 10배 이상 함유되어 있어 혈압강하 및 항산화 효과가 우수 한 것으로 알려져 있다 10,11) . 또한 뽕잎은 중성지방과 low density lipoprotein-콜레스테롤 수준을 감소시키는 등 지질 대사를 개선시키며 공복 혈당을 낮춘다 12,13) .…”

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Optimization of Mixing Ratio of Mulberry Leaf, Mulberry Fruit, and Silkworm for Amelioration of Metabolic Syndrome

Kim

2018

JKOMOR

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The aim of this study was optimized mixing ratio of mulberry leaf, mulberry fruit, and silkworm for amelioration of the metabolic syndrome by using response surface method (RSM). Methods: Antioxidant, antidiabetic and antihypertensive activities of fifteen mixed powder of mulberry leaf, mulberry fruit, and silkworm by RSM were measured as indicators of metabolic syndrome. Results: The optimal mixing ratio of mulberry leaves, mulberry fruits, and silkworm with the greatest antioxidant, antidiabetic and antihypertensive activities was as follows: 2.5890 of mulberry leaf (A), 0.1222 of mulberry fruit (B), 2.9999 of silkworm (C). At this time, it was predicted that the total polyphenol content was estimated to be 185.51 tannic acid equivalent mg/g, 1, 1-diphenyl-2-picrylhy drazyl radical scavenging activity 84.77%, 1-deoxynojirimycin content 415.66 mg/100 g, α-glucosidase inhibitory activity 64.31%, γ-aminobutyric acid content 267.77 mg/100 g, potassium content 1,899.11 mg/100 g, and angiotensin-converting-enzyme inhibitory activity was estimated to be 73.78%. Conclusions: It was concluded that the significant effect of the mulberry leaf, mulberry fruit, and silkworm on the metabolic syndrome-related biological activity indices (antioxidant activity, antidiabetic activity and antihypertensive activity) was as follows: 2.5890 of mulberry leaf (A), 0.1222 of mulberry fruit (B), 2.9999 of silkworm (C).

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Hypoglycemic Effect of Fermented Soybean Culture Mixed with Mulberry Leaves on Neonatal Streptozotocin-Induced Diabetic Rats

Cited by 15 publications

References 31 publications

Anti-hyperlipidemic effect of soybean extract fermented byBacillus subtilisMORI indb/dbmice

Anti-hyperlipidemic effect of soybean extract fermented byBacillus subtilisMORI indb/dbmice

Identification of the genes involved in 1-deoxynojirimycin synthesis in Bacillus subtilis MORI 3K-85

Optimization of Mixing Ratio of Mulberry Leaf, Mulberry Fruit, and Silkworm for Amelioration of Metabolic Syndrome

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