Hypoglycemic effect of polysaccharides with different molecular weight of Pseudostellaria heterophylla

Abstract

AbstractsBackgroundThe aims of this study were to evaluate the antidiabetic activity and to detect molecular size of Pseudostellaria heterophylla polysaccharide (PHP). Pseudostellaria heterophylla is a medicine extensively used in traditional Chinese medicine formulas to treat diabetes and its complications.MethodsMolecular weight of PHP was determined by gel permeation chromatography combined with phenol-sulphuric acid method and the monosaccharides composition was determined by HPLC with a precolumn derivatization. Four polysaccharides with different molecular weight were compared for hypoglycemic active on two animal models both high does alloxan induced type1 diabetic mellitus (T1DM) and high-fat/lower does streptozotocin induced type2 diabetic mellitus (T2DM). Blood sugar, glucose tolerance, and insulin tolerance were detected. Rat serum IL-1β, IL-2, IL-10, Leptin, TNF-α, Acrp30 and CRP were also analyzed by sandwich-ELISA approaches to preliminary probe the hypoglycemic mechanism of PHP.ResultsThe hypoglycemic effects related to molecular size of polysaccharide were more effective against T2DM than T1DM. PHP comprise four monosaccharides of galacturonic acid, glucose, galactose and arabinos. T2DM rats daily receiving oral dose of polysaccharide(100 ~ 400 mg/kg) with 50 ~ 210 kDa molecular weight (PF40) could not only significantly lower blood sugar but also reduce total triglyceride level in serum. PF40 improves in insulin tolerance inhibited the expression of some biomarkers including inflammatory cytokine TNF-α and elevated anti-inflammatory cytokine IL-10, regulated adiponectin Acrp30 and leptin.ConclusionsPF40 prevent the cascade of inflammatory events in the treatment of T2DM to block overweight progresses to obesity.

Structural Elucidation of a Novel Polysaccharide from Pseudostellaria heterophylla and Stimulating Glucose Uptake in Cells and Distributing in Rats by Oral

“…Raw polysaccharide was extracted from Pseudostellaria heterophylla using hot water extraction methods [11]. After this, it was treated with deproteinization, water dialysis, DEAE-cellulose 52, and Sephacryl S-300 column chromatography grading.…”

“…PF40, an anti-T2DM active fraction of polysaccharide from TZS was extracted according to literature by the authors' research group [11]. Fraction H-1 (757 mg) and H-2 (130 mg) were eluted with water from PF40 (7 g) using first-step DEAE-cellulose 52 column chromatography; a novel polysaccharide, designated as H-1-2, was eluted with water from H-1 using a second-step Sephacryl S-300 propylene dextran gel column.…”

“…T2DM rats receiving daily oral doses of polysaccharides (100~400 mg/kg), 50~210 kDa in molecular weight (named PF40), could, not only significantly lower blood sugar, but also reduce total triglyceride levels in serum, and improve insulin tolerance, regulated by the expression of some biomarkers, including TNF-α (Tumor necrosis factor-α), IL-10 (Interleukin-10), Acrp30 (Adipocyte complement-related protein of 30 kDa), etc. [11]. PF40 was the active anti-T2DM fraction of the polysaccharide.…”

Novel Polysaccharide H-1-2 from Pseudostellaria Heterophylla Alleviates Type 2 Diabetes Mellitus

“…Until recently, the potential therapeutic values of pseudostellaria heterophylla against diabetes have increasingly exploited. For example, Hu et al demonstrated the hypoglycemic effect of polysaccharides with different molecular weight and characterized the PF40 fraction effectively prevented the cascade of inflammatory events and blocked overweight progressing to obesity [13]. Chen et al isolated and identified a pectic polysaccharide in the aqueous phase of pseudostellaria heterophylla with potency to stimulate insulin secretion of INS-1 cell [20].…”

“…Three groups of rats, with 12 rats in each group, including normal control, diabetic with vehicle and diabetic with H-1-2 were subjected to either vehicle or H-1-2 administrations. The H-1-2 was dosed via oral gavage at 1.5 g/kg body weight for consecutive 30 days and controlled by drinking water, according to previously established method [13].…”