Hypoglycemic effects of space‐induced Lactobacillus plantarum SS18‐5 on type 2 diabetes in a rat model

Abstract

Probiotics can improve dyslipidemia and promote metabolic control as a therapeutic approach for type 2 diabetes mellitus (T2DM). The hypoglycemic effects of space‐induced Lactobacillus plantarum SS18‐5 on T2DM were explored in 4‐week‐old male Sprague Dawley rats. The normal (N) group was fed a basal diet, while the other groups received a high glucose fat diet. T2DM was established by streptozotocin injection and the T2DM rats were randomly divided into three groups, a diabetic (D) group (T2DM rats treated with saline only), GS18 group (T2DM rats treated with 109 CFU/ml of L. plantarum GS18), and SS18‐5 group (T2DM rats treated with 109 CFU/ml of L. plantarum SS18‐5). After continuous gavage for 6 weeks, blood biochemical indices were measured and livers were collected for histopathological examination. The colon contents were collected for counting of Escherichia coli, Clostridium perfringens, and Lactobacillus sp. The results showed that L. plantarum SS18‐5 effectively controlled the weight of rats, reduced levels of fasting blood glucose, glycosylated hemoglobin, and insulin, increased liver glycogen levels, improved abnormal metabolism of blood lipids, enhanced the effect of anti‐lipid peroxidation, alleviated chronic inflammation and fatty liver disease, and regulated the intestinal microbiota by reducing the numbers of E. coli and C. perfringens, and increasing the numbers of Lactobacillus sp. From these results, we conclude that space‐induced L. plantarum SS18‐5 has the potential to improve T2DM by alleviating hypoglycemia and regulating the intestinal microbiota. Practical applications With the exploration of the universe, a large number of studies have observed the changes of microorganisms in space flight, which provided a new method for high‐quality microbial pharmaceuticals in the space environment. In this study, the space environment mutated. Lactobacillus plantarum SS18‐5 can effectively improve the blood glucose of rats with type 2 diabetes, relieve oxidative stress, reduce blood lipid content, enhance immune capacity, and regulate intestinal microflora, which has potential use in the treatment of type 2 diabetes.

Evaluation of Hypoglycemic and Antioxidant Activities of Soybean Meal Products Fermented by Lactobacillus plantarum FPS 2520 and Bacillus subtilis N1 in Rats Fed with High-Fat Diet

“…It has been suggested that supplementation with probiotics has potential for improving type 2 diabetes, because probiotics are hypoglycemically active. For example, treatment with L. plantarum SS18–5 markedly improved the blood glucose of, and diminished oxidative stress in, diabetic rats [ 38 ]. Zhong et al substantiated the protective activity of L. plantarum ZJUFB2 from insulin resistance via modulating enteric microbiota and bile acids in HFD-treated mice [ 39 ].…”

Gestational Diabetes, Colorectal Cancer, Bariatric Surgery, and Weight Loss among Diabetes Mellitus Patients: A Mini Review of the Interplay of Multispecies Probiotics

“…This study, among obese rats ( n = 15), showed that probiotic supplements Lactobacillus acidophilus and Lactiplantibacillus plantarum , when administered orally, were effective in reducing the weight of obese rats. In addition, Lactiplantibacillus plantarum was proven effective in weight loss in Yang et al’s [ 71 ] research. In a similar vein of DM physiochemical parameters improvement was Hsieh et al’s [ 12 ] research among 50 DM rats, orally administered Liquorilactobacillus salivarius , Lactobacillus johnsonii , Limosilactobacillus reuteri , and Bifidobacterium animalis subsp.…”

The Space Environment Activates Capsular Polysaccharide Production in Lacticaseibacillus rhamnosus Probio-M9 by Mutating the wze ( ywqD ) Gene

“…A few studies have successfully applied space mutagenesis to improve the health-promoting effects of probiotic bacteria. For example, a space-exposed mutant, Lactiplantibacillus plantarum SS18-5, has shown effective hypoglycemic effects on type 2 diabetes in a rat model ( 14 ). Another space-exposed mutant, Limosilactobacillus reuteri F-9-35, has been found to enhance anti-gastric injury and anticolitis effects in rats ( 15 , 16 ).…”