Hypoglycemic effects of Tibetan medicine Huidouba in STZ-induced diabetic mice and db/db mice

Bai, Yinghui; Shi, Dong-xu; Lu, Hongbo; Yang, Kun-bao; Zhao, Huan-hu; Lu, Bingwei; Pang, Zhigang

doi:10.1016/j.chmed.2021.02.001

Cited by 13 publications

(8 citation statements)

References 7 publications

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“…39 In our current work, we assessed the serum lipid levels, insulin levels, and FBG levels tested in mice. The results showed that oral administration of 2.5 mg/kg PD to mice for 4 weeks could significantly reduce the levels of TC, TG, and LDL-C, increase the levels of HDL-C and INS, and inhibit the increase of FBG levels, which was consistent with the findings of Bai et al 40 cTnT and CK-MB are the two primary markers utilized for assessing the extent of myocardial injury. cTnT is primarily responsible for maintaining myocardial contraction and relaxation and exclusively exists in cardiomyocytes.…”

Section: Discussionsupporting

confidence: 89%

Platycodin D Ameliorates Type 2 Diabetes-Induced Myocardial Injury by Activating the AMPK Signaling Pathway

Wang,

Meng

et al. 2024

J. Agric. Food Chem.

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The current study aimed to assess the effectiveness of pharmacological intervention with Platycodin D (PD), a critically active compound isolated from the roots of Platycodon grandiflorum, in mitigating cardiotoxicity in a murine model of type 2 diabetes-induced cardiac injury and in H9c2 cells in vitro. Following oral administration for 4 weeks, PD (2.5 mg/kg) significantly suppressed the elevation of fasting blood glucose (FBG) levels, improved dyslipidemia, and effectively inhibited the rise of the cardiac injury markers creatine kinase isoenzyme MB (CK-MB) and cardiac troponin T (cTnT). PD treatment could ameliorate energy metabolism disorders induced by impaired glucose uptake by activating AMPK protein expression in the DCM mouse model, thereby promoting the GLUT4 transporter and further activating autophagy-related proteins. Furthermore, in vitro experiments demonstrated that PD exerted a concentration-dependent increase in cell viability while also inhibiting palmitic acid and glucose (HG-PA)-stimulated H9c2 cytotoxicity and activating AMPK protein expression. Notably, the AMPK activator AICAR (1 mM) was observed to upregulate the expression of AMPK in H9c2 cells after high-glucose and -fat exposure. Meanwhile, we used AMPK inhibitor Compound C (20 μM) to investigate the effect of PD activation of AMPK on cells. In addition, the molecular docking approach was employed to dock PD with AMPK, revealing a binding energy of −8.2 kcal/mol and indicating a tight interaction between the components and the target. PD could reduce the expression of autophagy-related protein p62, reduce the accumulation of autophagy products, promote the flow of autophagy, and improve myocardial cell injury. In conclusion, it has been demonstrated that PD effectively inhibits cardiac injury-induced type 2 diabetes in mice and enhances energy metabolism in HG-PA-stimulated H9c2 cells by activating the AMPK signaling pathway. These findings collectively unveil the potential cardioprotective effects of PD via modulation of the AMPK signaling pathway.

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Section: Discussionsupporting

confidence: 89%

Platycodin D Ameliorates Type 2 Diabetes-Induced Myocardial Injury by Activating the AMPK Signaling Pathway

Wang,

Meng

et al. 2024

J. Agric. Food Chem.

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“…Setelah 30 menit tikus diinduksi menggunakan amylum manihot 3 g/kgBB. Parameter yang diamati yaitu kadar glukosa darah pada T 30 , T 60, T 90 dan T 120 (10,11).…”

Section: Tes Toleransi Glukosa Oral Induksi Amylum Manihotunclassified

Potensi Penghambatan Enzim Αlfa-Glukosidase dan Tes Toleransi Glukosa Oral (TTGO) Ektrak Daun Dadap Serep (Erythrina subumbrans (Hassk) Merr.)

Susilawati

Astuti

Suprijono

2023

JFI

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Abstrak: Tanaman dadap serep memiliki kandungan flavonoid yang berpotensi dapat menurunkan kadar glukosa dalam darah dan mengurangi penyerapan glukosa di usus. Tujuan penelitian untuk mengetahui aktivitas penghambatan enzim α-glukosidase dan pengaruh pemberian Ekstrak Etanol Daun Dadap Serep (EEDDS) terhadap kadar glukosa darah tikus putih jantan galur wistar serta dosis efektifnya. Metode penelitian menggunakan metode in vitro dengan penghambatan enzim α-glukosidase dan metode in vivo Tes Toleransi Glukosa (TTGO) dengan induksi amylum manihot menggunakan 25 ekor tikus yang dibagi menjadi 5 kelompok dengan dosis EEDDS 100, 200, dan 400 mg/kgBB, kemudian diukur kadar glukosa darahnya selama 120 menit. Hasil penelitian inhibisi enzim α-glukosidase EEDDS menunjukkan nilai % inhibisi paling baik yaitu 41,81% pada 100 ppm dan nilai % inhibisi terendah pada 10 ppm yaitu 13,33% dengan nilai IC50 sebesar 146,30 ppm dimana termasuk kategori lemah dalam menghambat α-glukosidase. Hasil penelitian TTGO induksi amylum manihot, EEDDS 400 mg/KgBB memiliki efek penurunan kadar glukosa darah secara signifikan pada T60 sampai T120. . Kesimpulan pada penelitian ini adalah ekstrak etanol daun dadap serep mempunyai potensi sebagai antihiperglikemia secara in vitro maupun in vivo.

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“…As a population of immune cells in peripheral blood, PBMCs consist of various cell types, including lymphocytes, monocytes, and natural killer cells [ 8 ]. Comprehensive analysis of PBMCs from diabetic patients enables exploration of key features such as immune-metabolic dysregulation, inflammatory responses [ 9 ], and cellular functional changes associated with diabetes [ 10 ]. Given the current fatality rate of OC and the prevalence of diabetes, researchers and clinicians are increasingly interested in investigating whether the presence of DM in OC patients contributes to further disease exacerbation and poorer outcomes for both OC and DM.…”

Section: Introductionmentioning

confidence: 99%

Single-cell and transcriptomic analyses reveal the influence of diabetes on ovarian cancer

Zhao,

Wang,

Zhao

et al. 2024

BMC Genomics

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Background There has been a significant surge in the global prevalence of diabetes mellitus (DM), which increases the susceptibility of individuals to ovarian cancer (OC). However, the relationship between DM and OC remains largely unexplored. The objective of this study is to provide preliminary insights into the shared molecular regulatory mechanisms and potential biomarkers between DM and OC. Methods Multiple datasets from the GEO database were utilized for bioinformatics analysis. Single cell datasets from the GEO database were analysed. Subsequently, immune cell infiltration analysis was performed on mRNA expression data. The intersection of these datasets yielded a set of common genes associated with both OC and DM. Using these overlapping genes and Cytoscape, a protein‒protein interaction (PPI) network was constructed, and 10 core targets were selected. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were then conducted on these core targets. Additionally, advanced bioinformatics analyses were conducted to construct a TF-mRNA-miRNA coregulatory network based on identified core targets. Furthermore, immunohistochemistry staining (IHC) and real-time quantitative PCR (RT-qPCR) were employed for the validation of the expression and biological functions of core proteins, including HSPAA1, HSPA8, SOD1, and transcription factors SREBF2 and GTAT2, in ovarian tumors. Results The immune cell infiltration analysis based on mRNA expression data for both DM and OC, as well as analysis using single-cell datasets, reveals significant differences in mononuclear cell levels. By intersecting the single-cell datasets, a total of 119 targets related to mononuclear cells in both OC and DM were identified. PPI network analysis further identified 10 hub genesincludingHSP90AA1, HSPA8, SNRPD2, UBA52, SOD1, RPL13A, RPSA, ITGAM, PPP1CC, and PSMA5, as potential targets of OC and DM. Enrichment analysis indicated that these genes are primarily associated with neutrophil degranulation, GDP-dissociation inhibitor activity, and the IL-17 signaling pathway, suggesting their involvement in the regulation of the tumor microenvironment. Furthermore, the TF-gene and miRNA-gene regulatory networks were validated using NetworkAnalyst. The identified TFs included SREBF2, GATA2, and SRF, while the miRNAs included miR-320a, miR-378a-3p, and miR-26a-5p. Simultaneously, IHC and RT-qPCR reveal differential expression of core targets in ovarian tumors after the onset of diabetes. RT-qPCR further revealed that SREBF2 and GATA2 may influence the expression of core proteins, including HSP90AA1, HSPA8, and SOD1. Conclusion This study revealed the shared gene interaction network between OC and DM and predicted the TFs and miRNAs associated with core genes in monocytes. Our research findings contribute to identifying potential biological mechanisms underlying the relationship between OC and DM.

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Hypoglycemic effects of Tibetan medicine Huidouba in STZ-induced diabetic mice and db/db mice

Cited by 13 publications

References 7 publications

Platycodin D Ameliorates Type 2 Diabetes-Induced Myocardial Injury by Activating the AMPK Signaling Pathway

Platycodin D Ameliorates Type 2 Diabetes-Induced Myocardial Injury by Activating the AMPK Signaling Pathway

Potensi Penghambatan Enzim Αlfa-Glukosidase dan Tes Toleransi Glukosa Oral (TTGO) Ektrak Daun Dadap Serep (Erythrina subumbrans (Hassk) Merr.)

Single-cell and transcriptomic analyses reveal the influence of diabetes on ovarian cancer

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