Hypoglycemic, Hypolipidemic and Antioxidant Effects of Peptides from Red Deer Antlers in Streptozotocin-Induced Diabetic Mice

Abstract

Diabetes mellitus is a serious chronic metabolic disorder. To develop novel anti-diabetic drugs from nature sources has always been the focus of research. Red deer (Cervus elaphu Linnaeus) antler is one of the most famous Chinese traditional medicines. We found that the peptides of 5-10 kDa from red deer antlers (PRDA) promoted the growth of cultured rat islet cells. The purpose of this study was to investigate the anti-diabetic actions of PRDA in vivo and purify a pure active peptide. We therefore investigated the hypoglycemic, hypolipidemic and antioxidant effects of PRDA in streptozotocin-induced diabetic mice and isolated a pure anti-diabetic peptide. PRDA, given intraperitoneally (75, 150, or 300 μg/kg), significantly decreased the blood glucose levels, significantly increased the insulin concentrations, and remarkably improved the lipid metabolism in the diabetic mice. PRDA significantly increased the superoxide dismutase activity, catalase activity and the total antioxidant capacity in the serum and liver, and simultaneously decreased the malondialdehyde levels. The activities of hexokinase and pyruvate kinase, two important enzymes involved in glucose utilization, were also significantly increased in the liver of the PRDA-treated diabetic mice. Moreover, a novel anti-diabetic peptide isolated from PRDA significantly promoted the viability of cultured rat insulinoma cells. The molecular mass of the purified peptide was 7064.8 Da under mass spectrometry, and its N-terminal amino acid sequence was identified as LSPFTTKTYFPHFDLSHGSA. Thus, PRDA may be useful in managing the hyperglycemia, hyperlipidemia, and oxidative stress in diabetes, and the anti-diabetic peptide is a promising drug for the treatment of diabetes.

Antidiabetic and Antioxidant Impacts of Desert Date (Balanites aegyptiaca) and Parsley (Petroselinum sativum) Aqueous Extracts: Lessons from Experimental Rats

“…In the present study, the mean plasma TAC level in the DC group was significantly decreased in comparison with the NC group ( Figure 5 ). This finding coincides with the other studies which reported that diabetes was associated with reduced levels of antioxidant activity in the blood and tissues of diabetic animals [ 40 , 41 ]. The reduced mean plasma TAC level in the DC group might indicate that there was an imbalance between free radical formation and antioxidant protection as a consequence of increased utilization of endogenous antioxidants in response to the elevated levels of free radicals [ 16 ].…”

Hypolipidaemic and antioxidant capacities of polysaccharides obtained from Laminaria japonica by different extraction media in diet‐induced mouse model

“…Dietary antioxidants offer protection against ROS-/RNS-induced stress and damage through their direct actions based on mechanisms complementary to the functions of enzymatic antioxidants in the human body as well as their close association and support to primary and secondary enzymes (Durackova, 2010). Among these antioxidant species, antioxidative polysaccharides extracted from various raw materials in nature and their derived products have attracted increasing attention (Li et al, 2014;Jiang et al, 2015;Rangika et al, 2015). Among these antioxidant species, antioxidative polysaccharides extracted from various raw materials in nature and their derived products have attracted increasing attention (Li et al, 2014;Jiang et al, 2015;Rangika et al, 2015).…”

“…There are many dietary antioxidants that can be sourced from nature including protein-, polysaccharide-or lipid-based substances and small molecule phytochemicals such as vitamins and polyphenols (Lobo et al, 2010;Nimse & Pal, 2015). Among these antioxidant species, antioxidative polysaccharides extracted from various raw materials in nature and their derived products have attracted increasing attention (Li et al, 2014;Jiang et al, 2015;Rangika et al, 2015).…”

Increase of anticoagulant and fibrinolytic activity of rat blood plasma when using products of velvet antler industry

“…Помимо антиоксидантного эффекта пептидов, выделенных из пантов марала, были изучены также гипогликемические и гиполипидемические эффекты продуктов пантового оленеводства [5]. Показано, что компоненты продуктов пантового олене-водства оказывают иммуномодулирующее действие [6], также был отмечен гемопоэти-ческий эффект [7].…”