Hypolipidemic Activity and Antiatherosclerotic Effect of Polysaccharide ofPolygonatum sibiricumin Rabbit Model and Related Cellular Mechanisms

Abstract

Objective. To evaluate the hypolipidemic activity and antiatherosclerotic effect of polysaccharide of Polygonatum sibiricum (PPGS), which is a kind of Chinese herbal medicine using the rhizome part of the whole herb. Materials and Methods. Thirty rabbits were divided into normal control group, model control group, and PPGS subgroups of 0.8, 1.6, and 3.2 mL/kg/day under random selection. In atherosclerosis model, the effects of PPGS on diverse blood lipids, foam cells number, and aortic morphology were evaluated. In the primary culture of endothelial cells (ECs), the activities of PPGS on both ECs proliferation and ECs injury were studied as well. Results. In atherosclerosis model, the hypolipidemic activities of PPGS were mainly focused on TC, LDL-C, and Lp(a). All changes on these factors were statistically significant compared with model group (P < 0.01), except TG and HDL-C. The intimal foam cell number of PPGS subgroups (0.8, 1.6, and 3.2 mL/kg/day) was significantly reduced than model control (P < 0.01). In the primary culture of endothelial cells (ECs), PPGS showed no effect on cell proliferation but preferred to protect EC from injury and apoptosis induced by H2O2 and lipopolysaccharide (LPS). Discussion and Conclusion. The antiatherosclerotic effect of PPGS may be supported by its hypolipidemic activities, improving aortic morphology, and reducing foam cells number and ECs injury.

A Review: The Bioactivities and Pharmacological Applications of Polygonatum sibiricum polysaccharides

“…Some studies confirmed that the hypolipidemic activity of PSP was mainly due to modulation of TC, low-density lipoprotein (LDL-C), and lipoprotein (Lp (a)) in an atherosclerosis model. [ 16 ] PSP significantly reduced the intimal foam cells number compared to the control group. According to a study in endothelial cells (ECs), PSP not only affected EC proliferation, but also protected ECs from injury and apoptosis induced by H 2 O 2 and lipopolysaccharide (LPS).…”

“…PSP were found to be the major contributors to the sweet taste of PS which makes the food more readily accepted [ 14 ]. At present, PSP are widely used in TCM therapy for cardiovascular diseases and other diseases [ 15 , 16 , 17 , 18 , 19 , 20 ]. In conclusions, PSP play an important role in PS, which is commonly used in people’s lives.…”

Original Research: Potential ocular protection and dynamic observation of Polygonatum sibiricum polysaccharide against streptozocin-induced diabetic rats’ model

“…A total of 80 male Sprague Dawley (SD) rats (200 AE 20 g, two months old) were purchased from SPF (Beijing) Experimental Animal Technology Co. Ltd. All rats were housed under controlled conditions (22)(23)(24) C, 12:12 h light/dark cycle) and provided with food and water ad libitum. All experiments were approved by the laboratory animals' ethical committee of Taishan Medical University and followed national guidelines for the care and use of animals.…”

De Novo Assembly and Analysis of Polygonatum sibiricum Transcriptome and Identification of Genes Involved in Polysaccharide Biosynthesis

“…Polysaccharides isolated from P. sibiricum (PSPs) are major contributors to sweet taste and have many important biological functions [ 3 ]. The results of animal and cell experiments have shown that these compounds can promote the proliferation and enhance the viability of mesenchymal stem cells in bones [ 4 ], help prevent Alzheimer’s disease [ 5 ] and osteoporosis [ 6 ], serve as hypolipidemics and antiatherosclerotics [ 7 ], and improve immunologic functions [ 8 ]. Their polysaccharide structures and composition are closely related to their biological functions.…”