Hypolipidemic activity of Dracocephalum kotschyi involves FOXO1 mediated modulation of PPARγ expression in adipocytes

Abstract

BackgroundDracocephalum kotschyi, as a wild-growing flowering plant (from Lamiaceae family), is locally prescribed for its various health-promoting properties including hypolipidemic and hypoglycemic effects. To evaluate the scientific basis of the traditional use of Dracocephalum kotschyi extract (DKE), we aimed to disclose its mode of action with main focus on white adipose tissue of diabetic rats.MethodsStreptozotocin-induced diabetic rats were exposed to different doses of DKE for 28 days followed by the determination of the sera biochemical factors. The oxidative stress status of the diabetic versus nondiabetic rats’ adipose tissue under the influence of DKE were also evaluated in terms of malondialdehyde (MDA) and some of antioxidant enzymes (superoxide dismutase, SOD, and catalase). Furthermore, we exposed 3T3-L1 cells to DKE and then evaluated both the extent of cells differentiation to adipocytes and measured the expression levels of some of the key signaling elements involved in adipogenesis and lipogenesis with main focus on PPARγ.ResultsOur results indicated that DKE administration attenuated the levels of TG (triglycerides), TC (total cholesterol), LDL and blood glucose by 54, 40, 54 and 25%, respectively and enhanced the levels of HDL, catalase and SOD by 45, 74 and 56%, respectively. In addition to profound adipogenic and lipogenic effects on 3T3-L1 cells, DKE significantly enhanced p-AKT, p-FOXO1, PPARγ and SREBP-1 expressions while that of p-JNK was quenched parallel to effect of pioglitazone, an antidiabetic agent, used in our investigation as the positive control drug.ConclusionsBesides of confirming the hypolipidemic action of the plant, our results provided documents on at least one mode of action of DKE with profound effect on lipid metabolism in adipose tissue. Regarding our results, further investigation on DKE, as a new potential hypolipidemic alternative drug is warranted.Electronic supplementary materialThe online version of this article (10.1186/s12944-018-0893-3) contains supplementary material, which is available to authorized users.

Assessment of in vitro anti-skin aging activities of Phyllanthus indofischeri Bennet extracts for dermatological and aesthetic applications

“…The Prunus mume seed extract can increase the level of collagen and the SIRT1 mRNA expression and decreases the metalloproteinase 1 mRNA expression on mouse back dorsal skin tissue 5 . The extract from Dracocephalum kotschyi significantly up-regulates p-AKT, p-FOXO1, PPAR, and SREBP-1 expressions in adipose tissue 45 . The extracts of Retama monosperma (L.) Boiss.…”

Assessment of in vitro anti-skin ageing activities of Giant Indian Gooseberry (Phyllanthus indofischeri Bennet) extracts for dermatological health and aesthetic applications

“…The Prunus mume seed extract can increase the level of collagen and the SIRT1 mRNA expression and decreases the metalloproteinase 1 mRNA expression on mouse back dorsal skin tissue 5 . The extract from Dracocephalum kotschyi signi cantly up-regulates p-AKT, p-FOXO1, PPAR, and SREBP-1 expressions in adipose tissue 42 . The extracts of Retama monosperma (L.) Boiss.…”

The effect of Dracocephalum subcapitatum hydroalcoholic extract on dexamethasone-induced hyperlipidemic rats

“…There are several reports about the useful effects of some Drcacephalum plants in improving hyperglycemia and hyperlipidemia ( 31 32 33 ). D. kotschyi is a well-known species in this genus with various therapeutic properties including blood lipid-lowering effect.…”

“…Aslian et al . reported that D. kotschyi extract decreased blood levels of triglycerides (54%), total cholesterol (40%), LDL (54%), and blood glucose (25%) and increased HDL (45%) in adipose tissues in diabetic rats ( 32 ). In their in vitro study, D. kotschyi extract augmented expression of some crucial genes involved in lipid metabolism and adipogenesis including peroxisome proliferator-activated receptor gamma (PPARγ), sterol regulatory element-binding type 1 (SREBP-1), forkhead box O-1 (FOXO1), and protein kinase B while suppressed c-Jun N-terminal kinase (JNK) in 3T3-L1 adipocyte cells ( 32 ).…”