Hypomontagnella (Hypoxylaceae): a new genus segregated from Hypoxylon by a polyphasic taxonomic approach

Discovery of a new species of the Hypoxylon rubiginosum complex from Iran and antagonistic activities of Hypoxylon spp. against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in dual culture

“…Kuhnert et al (2014) also found tub2 to be more suitable than ITS in their phylogeny, based on material from the Caribbean. Our phylogenetic analyses confirmed previous results (Wendt et al 2018;Lambert et al 2019;Sir et al 2019), suggesting that the genus Hypoxylon appears paraphyletic in Hypoxylaceae, with a relatively small clade comprising the type species H. fragiforme as "core group" to which members of the Hypoxylon rubiginosum complex form a sister clade. The genus will eventually need to be further subdivided, but molecular data for the majority of known species remain incomplete and such a task should only commence as the phylogenetic data matrix has increased.…”

“…It traditionally belonged to the Xylariaceae until a recent phylogenetic study has resulted in a re-arrangement of the genera of stromatic Xylariales and the resurrection of the family Hypoxylaceae (Wendt et al 2018). In this study and during the followup work by Lambert et al (2019), genera like Hypomontagnella and Pyrenopolyporus were segregated from Hypoxylon, but the genus remained paraphyletic, indicating that further taxonomic segregation will eventually become necessary.…”

Observations on Texas hypoxylons, including two newHypoxylonspecies and widespread environmental isolates of theH. croceumcomplex identified by a polyphasic approach

“…The new sequences of each locus were aligned with 49 corresponding Hypoxylaceae sequences and a Xylaria hypoxylon sequence as outgroup by using 100 iterations of MUSCLE alignment software (Edgar 2004) (for sequence details see TABLE 1). For the phylogenetic assessment of sequence data the general procedure of Lambert et al (2019) was followed. Alignment data generated by a fast Fourier transformation approach (MAFFT) was processed via Geneious® 7.1.9 (http://www.geneious.com) and subjected to a curation of phylogenetic informative characters via the Castresana Lab Gblocks Server (Talavera and Castresana 2007).…”

“…The Hypoxylaceae with its approximately 400 described species placed into 16 genera accounts for a vast portion of the taxonomic diversity within the order. The largest genus in the Hypoxylaceae is the type genus Hypoxylon with around 180 known members (Lambert et al 2019, Wendt et al 2018.…”

The sporothriolides. A new biosynthetic family of fungal secondary metabolites

“…Sporochartines 6a-6d 8,9 from the fungus Hypoxylon monticulosum CLL 205 (now referred to as Hypomontagnella spongiphila) 10 possesses potent cytotoxicity (IC 50 : 7.2 to 21.5 mM) vs. human cancer cell lines and are proposed to be Diels Alder (DA) adducts of the furofurandione sporothriolide 1, itself a potent antifungal agent (EC 50 : 11.6 AE 0.8 mM), 11 and trienylfuranol A 7, 12 originally obtained from an endophytic fungus Hypoxylon submonticulosum DAOMC 242471 (now referred to as Hypomontagnella submonticulosa). 13 Since the biosynthesis of sporothriolide 1 and related compounds is unknown, and biological DA reactions in fungi are currently of high interest, 14 we decided to examine the biosynthesis of the sporochartines 6 in the Hypomontagnella spp. strains MUCL 54604 and CLL 205 (ref.…”