Hypotensive and endothelium-independent vasorelaxant effects of methanolic extract from Curcuma longa L. in rats

Adaramoye, Oluwatosin A.; Anjos, Raline Mendonça dos; Almeida, Mônica Moura de; Veras, Robson Cavalcante; Silvia, Darizy Flavia; Oliveira, Francisco de Assis; Cavalcante, Karla Veruska Marques; Araújo, Islania Giselia Albuquerque; Oliveira, Aldeídia Pereira de; Medeiros, Isac A.

doi:10.1016/j.jep.2009.05.021

Cited by 57 publications

(31 citation statements)

References 36 publications

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“…In a previous study, Sasaki et al, (2003) reported the endothelium-independent vasomotional effects of five Curcuma drugs (Curcuma longa inclusive) in isolated rat aorta. Recently, it was demonstrated that CL lowers arterial blood pressure and heart rate in rats, probably due to the blockade of extracellular Ca 2+ influx and, the inhibition of intracellular Ca 2+ release for inositol-1,4,5-triphosphate (IP3) sensitive stores (Adaramoye et al, 2008). These results provide enough background to explore the involvement of the Na + -Ca 2+ exchanger in the vasorelaxant effects induced by CL in isolated rat superior mesenteric arteries.…”

Section: Introductionmentioning

confidence: 91%

Involvement of Na+-Ca 2+ exchanger in the endothelium-independent vasorelaxation induced by Curcuma longa L. in isolated rat superior mesenteric arteries

Adaramoye

Medeiros

2008

J. Smooth Muscle Res.

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Previous studies confirmed that the methanolic extract from Curcuma longa L. (CLME) lowers arterial blood pressure and heart rate in rats due to the blockade of extracellular Ca 2+ influx. The aim of this study was to investigate the involvement of Na + -Ca 2+ exchanger in the vasorelaxant effects elicited by CLME in isolated rat superior mesenteric arteries. CLME (1-1,000 µg/ml) concentration-dependently relaxed phenylephrine (PHE) (10 µM) pre-contracted arterial rings with intact-endothelium (pD2 and Emax = 2.04 ± 0.06 and 88.3 ± 3.2%) or denuded-endothelium (pD2 and Emax = 2.06 ± 0.03 and 91.4 ± 1.0%), respectively, suggesting that the removal of endothelium has no significant effect (P>0.05) on the vasorelaxation induced by CLME. Furthermore, CLME (30, 100 and 300 µg/ml) inhibited the cumulative concentration-response curves to PHE (10 -8 -10 -5 M) in a concentration-dependent manner, whereas, treatment with ouabain 100 µM (selective blocker of Na + -K + ATPase) has no effect on the relaxant responses of CLME. However, treatment with nickel chloride (NiCl2) (100, 300 and 400 µM), a putative Na + -Ca 2+ exchanger inhibitor, concentration-dependently reduced the vasorelaxant responses of CLME. Precisely, NiCl2 at 100, 300 and 400 µM significantly (P<0.05) decreased the pD2 and Emax values of CLME (1.86 ± 0.03 and 81.3 ± 1.2%, 1.77 ± 0.03 and 60.2 ± 0.8%, 1.69 ± 0.04 and 55.3 ± 1.6%, respectively). Also, CLME (100 µg/ml) produced less relaxant effect with decreasing extracellular Na + concentration. CLMEinduced vasorelaxation was completely abolished in a Na + -free Tyrode's solution, a condition that eliminates the influence of the forward mode of the exchanger. The results provide indirect evidence that the stimulation of the forward mode of Na + -Ca 2+ exchanger may probably contribute to the vasorelaxation induced by CLME in endothelium-denuded arterial rings.

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Section: Introductionmentioning

confidence: 91%

Involvement of Na+-Ca 2+ exchanger in the endothelium-independent vasorelaxation induced by Curcuma longa L. in isolated rat superior mesenteric arteries

Adaramoye

Medeiros

2008

J. Smooth Muscle Res.

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“…Medicinal plants contain several classes of bioactive compounds such as polyphenols, alkaloids and terpenes (Oliveira et al, 1996;Barbosa-Filho et al, 2000;Adaramoye et al, 2009). Among the pharmacological activities of compounds isolated from medicinal drugs on the cardiovascular system are calcium antagonism, NO release, antioxidant and others (Guedes et al, 2004;Ribeiro et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Erythroxylum pungens elicits vasorelaxation by reducing intracellular calcium concentration in vascular smooth muscle cells of rats

Oliveira¹,

Sena-Filho²,

Mendes-Júnior³

et al. 2012

Rev. bras. farmacogn.

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Abstract:The cardiovascular effects elicited by the ethanolic extract obtained from the roots of Erythroxylum pungens O.E. Schulz, Erythroxylaceae (EEEP) and the vasorelaxant effect induced by its main tropane alkaloid (pungencine) were investigated. In normotensive rats, administration of EEEP (1, 10, 30 and 60 mg/kg i.v., randomly) produced dose-dependent hypotension (-2±1, -7±0.5 -17.6±1, -24±1 ∆ mmHg, n=5) followed by tachycardia (3±0.5, 7±2, 7.1±1, 10±5 ∆ bpm, n=5). In intact phenylephrine (Phe, 10 μM)-pre-contracted rings, EEEP (0.01-500 μg/mL) induced concentrationdependent vasorelaxation (EC50 13.7±5.5 μg/mL, Maximal Response= 92±2.6%), and this effect was unchanged after the removal of the vascular endothelium (EC50 27.2±4.7 μg/ml, Maximal Response= 88.3±3.3 %). In KCl (80 mM)-pre-contracted-endotheliumdenuded rings, EEEP elicited concentration-dependent relaxation (EC50= 128.2±11.2 μg/mL, Maximal Response 76.8±3.4%). Vasorelaxation has also been achieved with tonic contractions evoked by the L-type Ca 2+ channel agonist Bay K 8644 (EC50 80.2±9.1 μg/mL, Maximal Response 86.3±8.3%). In addition, in a depolarizing medium, EEEP inhibited CaCl 2 (30-500 μg/mL) induced contractions and caused a concentrationdependent rightward shift of the relaxation curves. Lastly, the tropane alkaloid pungencine caused vasorelaxation in mesenteric arteries resembling to the EEEP responses. These results suggests that EEEP induces hypotension and vasorelaxation, at least in part, due to the reduction in [Ca 2+ ]i in vascular smooth muscle cells.

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“…Accordingly, C. mukul resin was extracted using petroleum ether, 16 S. reticulata root using water, 17 bark of T. arjuna 18 and rhizome of C. longa using methanol. 19 Approximately, 20 g of each powder was extracted with 150 mL of respective solvent using a magnetic stirrer for 72 h; changing solvent every 24 h. Each time the supernatants obtained by centrifugation were collected. Finally the supernatants were pooled, concentrated and evaporated to dryness in a water bath.…”

Section: Hptlc Fingerprintingmentioning

confidence: 99%

Component Authentication and Standardisation of an Anti-atherosclerotic Herbal Formulation-GSTC3

Manalil¹,

Suseela²,

Ramavarma³

et al. 2015

Phcog J

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Context: Polyherbal drugs in traditional medicine have been time-tested for treatment of a wide variety of chronic ailments. Aims: To determine constituent components in an in-house designed polyherbal formulation GSTC3 and evaluation of consistency in preparation. Settings and Design: In this study, a hypolipidemic formulation was created using well known plant extracts such as Commiphora mukul (Hook. ex Stocks.) Eng. (Burseraceae), Salacia reticulata Wight (Celastraceae), Terminalia arjuna (Roxb.) Wight and Arn (Combretaceae) and Curcuma longa Linn (Zingiberaceae). Methods and Material:The authentication of individual plant powders was performed using techniques such as powder microscopy and characterised according to Ayurvedic pharmacopeia of India. Phytochemical screening, HPTLC analysis and physicochemical parameters were also determined. Results:The powder microscopic analysis of the individual components served as an authentication for source of plants used. Phytochemical screening ascertained that active classes of compounds reported in the individual extracts such as steroids, terpenoids and polyphenols came into the formulation. Finally, HPTLC analysis of three different batches of GSTC3 ensured stability and integrity in batch to batch preparations. Conclusion: Experimental studies have revealed the antioxidant, antiinflammatory, anti-lipidemic and anti-thrombotic efficacy of GSTC3. This standardisation procedure is essential for further development of GSTC3 into an efficient anti-atherosclerotic drug candidate.Key words: HPTLC, Pharmacognosy, Polyherbal formulation, Powder microscopy, Phytochemical screening. SUMMARY• GSTC is an anti-atherosclerotic polyherbal formulation developed from four individual medicinal plants.• Powder microscopic analysis of the individual components of GSTC3 served as an authentication of source plant.• Phytochemical screening ascertained the presence of steroids, terpenoids and polyphenols in the formulation.• HPTLC analysis of three different batches of GSTC3 ensured stability and integrity among batch to batch preparations.• Physicochemical analysis showed the feasibility of GSTC3 in its further development as an efficient anti-atherosclerotic drug candidate.

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Hypotensive and endothelium-independent vasorelaxant effects of methanolic extract from Curcuma longa L. in rats

Cited by 57 publications

References 36 publications

Involvement of Na+-Ca 2+ exchanger in the endothelium-independent vasorelaxation induced by Curcuma longa L. in isolated rat superior mesenteric arteries

Involvement of Na+-Ca 2+ exchanger in the endothelium-independent vasorelaxation induced by Curcuma longa L. in isolated rat superior mesenteric arteries

Erythroxylum pungens elicits vasorelaxation by reducing intracellular calcium concentration in vascular smooth muscle cells of rats

Component Authentication and Standardisation of an Anti-atherosclerotic Herbal Formulation-GSTC3

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