Hypotensive effect of aqueous extract of Averrhoa carambola L. (Oxalidaceae) in rats: An in vivo and in vitro approach

Soncini, Roseli; Santiago, Michael Brian; Orlandi, Lidiane; Moraes, Gabriel O. I.; Peloso, André Luiz M.; Santos, Marcelo H. dos; Alves-da-Silva, Geraldo; Paffaro, Valdemar A.; Bento, A. C.; Giusti‐Paiva, Alexandre

doi:10.1016/j.jep.2010.10.001

Cited by 45 publications

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“…Some species analyzed applying a similar assay used in the present study also showed hypotensive activity (Singi et al, 2005;Soncini et al, 2011). Soncini et al (2011) reported that aqueous extract of Averrhoa carambola L. (Oxalidaceae) showed a dosedependent pressure reduction.…”

Section: Resultssupporting

confidence: 60%

“…Soncini et al (2011) reported that aqueous extract of Averrhoa carambola L. (Oxalidaceae) showed a dosedependent pressure reduction. The hypotensive activity of hydroalcoholic extracts of garlic (Allium sativum L.) and lemon-grass (Cymbopogon citratus (DC) Stapf) were reported by Singi et al (2005).…”

Section: Resultsmentioning

confidence: 99%

“…The WKY and SHR animals received saline 1 mL/kg (Soncini et al, 2011) and, afterwards, increasing doses, in bolus, of ERE (0.01 -100 mg/kg). The subsequent dose was administrated only after MAP returned to the levels observed prior to the first application.…”

Section: Acute Hypotensive Assaymentioning

confidence: 99%

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In vivo hypotensive effect and in vitro inhibitory activity of some Cyperaceae species

Martins

Pacheco

Perini

et al. 2013

Braz. J. Pharm. Sci.

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In 1820, French naturalist August Saint Hillaire, during a visit in Espírito Santo (ES), a state in southeastern Brazil, reported a popular use of Cyperaceae species as antidote to snake bites. The plant may even have a hypotensive effect, though it was never properly researched. The in vitro inhibitory of the angiotensin converting enzyme (ACE) activity of eigth ethanolic extracts of Cyperaceae was evaluated by colorimetric assay. Total phenolic and flavonoids were determined using colorimetric assay. The hypotensive effect of the active specie (Rhychonospora exaltata, ERE) and the in vivo ACE assay was measured in vivo using male Wistar Kyoto (ERE, 0.01-100mg/kg), with acetylcholine (ACh) as positive control (5 µg/kg, i.v.). The evaluation of ACE in vivo inhibitory effect was performed comparing the mean arterial pressure before and after ERE (10 mg/kg) in animals which received injection of angiotensin I (ANG I; 0,03, 03 and 300 µg/kg, i.v.). Captopril (30 mg/kg) was used as positive control. Bulbostylis capillaris (86.89 ± 15.20%) and ERE (74.89 ± 11.95%, ERE) were considered active in the in vitro ACE inhibition assay, at 100 µg/mL concentration. ACh lead to a hypotensive effect before and after ERE's curve (-40±5% and -41±3%). ERE showed a dose-dependent hypotensive effect and a in vivo ACE inhibitory effect. Cyperaceae species showed an inhibitory activity of ACE, in vitro, as well as high content of total phenolic and flavonoids. ERE exhibited an inhibitory effect on both in vitro and in vivo ACE. The selection of species used in popular medicine as antidotes, along with the in vitro assay of ACE inhibition, might be a biomonitoring method for the screening of new medicinal plants with hypotensive properties.Uniterms: Cyperaceae/species/pharmacognosy. Cyperaceae/species/in vitro inhibitory activity. Cyperaceae/species/in vivo hypotensive effect. Angiotensin converting enzyme/in vitro inhibitory. Flavonoids/determination. Em 1820, o naturalista francês August Saint Hillaire, durante uma visita ao Espírito Santo (ES), Estado do sudeste do Brasil, relatou o uso popular de espécies de Cyperaceae como antídoto para picadas de cobra. As espécies podem possuir efeito hipotensor, embora nunca tenham sido devidamente pesquisadas. A inibição in vitro da atividade da enzima conversora da angiotensina (ECA) de oito extratos etanólicos de Cyperaceae foi avaliada por ensaio colorimétrico. Totais de fenólicos e flavonóides foram determinados utilizando ensaio colorimétrico. O efeito hipotensor da espécie ativa (Rhychonospora exaltata, ERE) no ensaio de ECA in vitro foi avaliada in vivo utilizando-se machos Wistar Kyoto (ERE, 0.01-100 mg/kg), com a acetilcolina (ACh), como controle positivo (5 µg/kg, iv). A avaliação do efeito inibidor da ECA in vivo foi realizado comparando-se a pressão arterial média, antes e após ERE (10 mg/kg) nos animais que receberam injeção de angiotensina I (Ang I, 0,03, 03 e 300 µg/kg, iv). Captopril (30 mg/kg) foi utilizado como controle positivo. Bulbostylis capillaris (86,89±15,20%) e ERE (7...

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Section: Resultssupporting

confidence: 60%

Section: Resultsmentioning

confidence: 99%

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In vivo hypotensive effect and in vitro inhibitory activity of some Cyperaceae species

Martins

Pacheco

Perini

et al. 2013

Braz. J. Pharm. Sci.

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“…WKY and SHR animals received saline (1 mL kg -1 ) as a negative control to establish a baseline (Soncini et al, 2011), and they then received increasing doses of the EEAP (0.1-100 mg kg -1 ) or IAP (0.01-10 μg kg -1 ), both dissolved in saline (0.9% NaCl). Subsequent doses were injected only after MAP levels returned to baseline.…”

Section: Evaluation Of the Acute Hypotensive Effects Of The Eeap And Iapmentioning

confidence: 99%

Hypotensive effect and endothelium-dependent vascular action of leaves of Alpinia purpurata (Vieill) K. Schum

Silva

Lima

Caliman

et al. 2014

Braz. J. Pharm. Sci.

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The aims of this study were to evaluate the chemical profile, vascular reactivity, and acute hypotensive effect (AHE) of the ethanolic extract of leaves of Alpinia purpurata (Vieill) K. Schum (EEAP). Its chemical profile was evaluated using HPLC-UV, ICP-OES, and colorimetric quantification of total flavonoids and polyphenols. The vascular reactivity of the extract was determined using the mesenteric bed isolated from WKY. AHE dose-response curves were obtained for both EEAP and inorganic material isolated from AP (IAP) in WKY and SHR animals. Cytotoxic and mutagenic safety levels were determined by the micronucleus test. Rutin-like flavonoids were quantified in the EEAP (1.8 ± 0.03%), and the total flavonoid and polyphenol ratios were 4.1 ± 1.8% and 5.1 ± 0.3%, respectively. We observed that the vasodilation action of EEAP was partially mediated by nitric oxide ( ). The EEAP and IAP showed an AHE in WKY and SHR animals. EEAP did not have cytotoxic effects or cause chromosomic alterations. The AHE shown by EEAP could result from its endothelium-dependent vascular action. Rutin-like flavonoids, among other polyphenols, could contribute to these biological activities, and the calcium present in EEAP could act in a synergistic way.Uniterms: Alpinia. Alpinia purpurata/phytochemistry. Alpinia purpurata/ethanolic extract/vascular reactivity. Alpinia purpurata/ethanolic extract/acute hypotensive effect. Flavonoids/quantification. Polyphenols/quantification.Os objetivos deste estudo foram avaliar o perfil químico de folhas de Alpinia purpurata K. Schum (AP), assim como a reatividade vascular e o efeito hipotensor agudo (EHA) do extrato etanólico de folhas de AP (EEAP). Avliou-se o perfil químico utilizando-se HPLC-UV, ICP-OES e quantificação colorimétrica de flavonoides e polifenóis totais. A reatividade vascular foi determinada utilizando leito mesentérico isolado de ratos WKY. Curvas dose-resposta do EEAP e do material inorgânico da AP (IAP) foram realizadas em animais SHR e WKY. Determinaram-se a segurança citotóxica e mutagênica pelo teste de micronúcleos. Flavonoides tipo rutina foram quantificados no EEAP (1,8±0,03%) e flavonoide total e polifenóis foram de 4,1±1,8% e 5,1±0,3%, respectivamente. Observou-se ação vasodilatadora do EEAP, mediada parcialmente pelo óxido nítrico (

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“…Some researches have shown that complementary and alternative medicine has potential in the treatment of hypertension 7,9 . The approval of complementary and alternative medicine has grown over the past two decades among physicians and patients alike 9,10 .…”

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confidence: 99%

Untitled

2017

IJPSR

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Aim:To evaluate the antihypertensive effect of ethanolic extract of seeds of Phaseolus vulgaris Linn. (EEPV) on high salt diet induced hypertension in male rats. Methods: Male wistar rats (180-200g) received high salt diet (8% NaCl) and 1% saline water for 6 weeks, then cannulated for dosing of Enalapril, angiotensin converting enzyme inhibitor (0.5 mg/kg, i.v.) in standard group and EEPV (50 and 100 mg/kg, i.v.) in test group and measured mean blood pressure, systolic blood pressure, diastolic blood pressure and heart rate with the help of Power Lab 6 Pro after 60 minutes of treatment. Results: Elevated blood pressure of the animals was significantly (P < 0.05) decreased by EEPV (100 mg/kg, i.v). Enalapril, at the dose of 0.5 mg/kg, i.v. showed significant (P < 0.05) reduction in the elevated blood pressure. The antihypertensive activity of ethanolic extract of seeds of Phaseolus vulgaris Linn. may be due to the action on rennin-angiotensin system. The antihypertensive activity of EEPV might also be explained by the presence of phytochemical constituents like flavanoids and saponins in the plant. Conclusion:The work demonstrated that the concomitant administration of highsalt and EEPV elicits prevention of high salt-induced hypertension in rats. Overall, results justify and support the use of Phaseolus vulgaris Linn. as antihypertensive medicine.

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Hypotensive effect of aqueous extract of Averrhoa carambola L. (Oxalidaceae) in rats: An in vivo and in vitro approach

Abstract: These results demonstrate the hypotensive effects of AEAc, and these effects may, in part, be due to the inhibition of Ca(2+), which supports previous claims of its traditional use.

Cited by 45 publications

References 31 publications

In vivo hypotensive effect and in vitro inhibitory activity of some Cyperaceae species

In vivo hypotensive effect and in vitro inhibitory activity of some Cyperaceae species

Hypotensive effect and endothelium-dependent vascular action of leaves of Alpinia purpurata (Vieill) K. Schum

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