Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat

Rey-Funes, Manuel; Larráyoz, Ignacio M.; Contartese, Daniela S; Soliño, Manuel; Sarotto, Aníbal; Bustelo, Martín; Bruno, Martín A.; Dorfman, Verónica Berta; Loidl, César Fabián; Martı́nez, Alfredo

doi:10.1038/s41598-017-07294-6

Cited by 25 publications

(29 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Fortyfive-day-old rats (n 10 per experimental group) were subjected to electroretinography, as described (Rey-Funes et al, 2017). Briefly, after overnight adaptation in the dark, rats were anesthetized under dim red illumination.…”

Section: Electroretinogramsmentioning

confidence: 99%

Hypothermic Shock Applied After Perinatal Asphyxia Prevents Retinal Damage in Rats

et al. 2021

Self Cite

View full text Add to dashboard Cite

Perinatal asphyxia (PA) can cause retinopathy and different degrees of visual loss, including total blindness. In a rat model of PA, we have previously shown a protective effect of hypothermia on the retina when applied simultaneously with the hypoxic insult. In the present work, we evaluated the possible protective effect of hypothermia on the retina of PA rats when applied immediately after delivery. Four experimental groups were studied: Rats born naturally as controls (CTL), animals that were exposed to PA for 20 min at 37°C (PA), animals exposed to PA for 20 min at 15°C (HYP), and animals that were exposed to PA for 20 min at 37°C and, immediately after birth, kept for 15 min at 8°C (HYP-PA). To evaluate the integrity of the visual pathway, animals were subjected to electroretinography at 45 days of age. Molecular (real time PCR) and histological (immunohistochemistry, immunofluorescence, TUNEL assay) techniques were applied to the eyes of all experimental groups collected at 6, 12, 24, and 48 h, and 6 days after birth. PA resulted in a significant reduction in the amplitude of the a- and b-wave and oscillatory potentials (OP) of the electroretinogram. All animals treated with hypothermia had a significant correction of the a-wave and OP, but the b-wave was fully corrected in the HYP group but only partially in the HYP-PA group. The number of TUNEL-positive cells increased sharply in the ganglion cell layer of the PA animals and this increase was significantly prevented by both hypothermia treatments. Expression of the cold-shock proteins, cold-inducible RNA binding protein (CIRP) and RNA binding motif protein 3 (RBM3), was undetectable in retinas of the CTL and PA groups, but they were highly expressed in ganglion neurons and cells of the inner nuclear layer of the HYP and HYP-PA groups. In conclusion, our results suggest that a post-partum hypothermic shock could represent a useful and affordable method to prevent asphyxia-related vision disabling sequelae.

show abstract

Section: Electroretinogramsmentioning

confidence: 99%

Hypothermic Shock Applied After Perinatal Asphyxia Prevents Retinal Damage in Rats

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…For instance, it has been widely reported that it is a relatively potent inhibitor of SCD (IC 50 = 0.9 uM). However, SA is also able to mediate anti-inflammatory and protective effects in the retina independently of SCD (AP, RP, APS, IML, in preparation) by a mechanism that could also involve the toll-like receptor 4 (TLR4) receptor and intracellular kinases [58] and could be of interest in other retinal pathologies [120,121].…”

Section: Sa and Therapeutic Opportunitiesmentioning

confidence: 99%

Sterculic Acid: The Mechanisms of Action beyond Stearoyl-CoA Desaturase Inhibition and Therapeutic Opportunities in Human Diseases

Peláez

Pariente

Pérez-Sala

et al. 2020

Cells

View full text Add to dashboard Cite

In many tissues, stearoyl-CoA desaturase 1 (SCD1) catalyzes the biosynthesis of monounsaturated fatty acids (MUFAS), (i.e., palmitoleate and oleate) from their saturated fatty acid (SFA) precursors (i.e., palmitate and stearate), influencing cellular membrane physiology and signaling, leading to broad effects on human physiology. In addition to its predominant role in lipid metabolism and body weight control, SCD1 has emerged recently as a potential new target for the treatment for various diseases, such as nonalcoholic steatohepatitis, Alzheimer’s disease, cancer, and skin disorders. Sterculic acid (SA) is a cyclopropene fatty acid originally found in the seeds of the plant Sterculia foetida with numerous biological activities. On the one hand, its ability to inhibit stearoyl-CoA desaturase (SCD) allows its use as a coadjuvant of several pathologies where this enzyme has been associated. On the other hand, additional effects independently of its SCD inhibitory properties, involve anti-inflammatory and protective roles in retinal diseases such as age-related macular degeneration (AMD). This review aims to summarize the mechanisms by which SA exerts its actions and to highlight the emerging areas where this natural compound may be of help for the development of new therapies for human diseases.

show abstract

“…In mammals, however, loss of temperature homeostasis, as occurs in hypo-or hyperthermia can have pathological effects on neuronal function, as occurs in febrile seizures in hyperthermia [10]. Experiments in human subjects have also shown that modest changes in body temperature appear able to alter visually-evoked potentials [11] and lowering body temperature has a neuroprotective effect on the survival and function of retinal neurons in a model of optic nerve injury [12].…”

Section: Introductionmentioning

confidence: 99%

Temperature effects on synaptic transmission and neuronal function in the visual thalamus

Hook

2020

PLoS ONE

View full text Add to dashboard Cite

Numerous neuronal properties including the synaptic vesicle release process, neurotransmitter receptor complement, and postsynaptic ion channels are involved in transforming synaptic inputs into postsynaptic spiking. Temperature is a significant influencer of neuronal function and synaptic integration. Changing temperature can affect neuronal physiology in a diversity of ways depending on how it affects different members of the cell's ion channel complement. Temperature's effects on neuronal function are critical for pathological states such as fever, which can trigger seizure activity, but are also important in interpreting and comparing results of experiments conducted at room vs physiological temperature. The goal of this study was to examine the influence of temperature on synaptic properties and ion channel function in thalamocortical (TC) relay neurons in acute brain slices of the dorsal lateral geniculate nucleus, a key synaptic target of retinal ganglion cells in the thalamus. Warming the superfusate in patch clamp experiments with acutely-prepared brain slices led to an overall inhibition of synaptically-driven spiking behavior in TC neurons in response to a retinal ganglion cell spike train. Further study revealed that this was associated with an increase in presynaptic synaptic vesicle release probability and synaptic depression and altered passive and active membrane properties. Additionally, warming the superfusate triggered activation of an inwardly rectifying potassium current and altered the voltage-dependence of voltage-gated Na + currents and T-type calcium currents. This study highlights the importance of careful temperature control in ex vivo physiological experiments and illustrates how numerous properties such as synaptic inputs, active conductances, and passive membrane properties converge to determine spike output.

show abstract

Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat

Cited by 25 publications

References 51 publications

Hypothermic Shock Applied After Perinatal Asphyxia Prevents Retinal Damage in Rats

Hypothermic Shock Applied After Perinatal Asphyxia Prevents Retinal Damage in Rats

Sterculic Acid: The Mechanisms of Action beyond Stearoyl-CoA Desaturase Inhibition and Therapeutic Opportunities in Human Diseases

Temperature effects on synaptic transmission and neuronal function in the visual thalamus

Contact Info

Product

Resources

About