Hypothermia reduces secretion of vascular endothelial growth factor by cultured retinal pigment epithelial cells

Coassin, Marco; Duncan, Keith G.; Bailey, Kathy; Singh, Ajay P.; Schwartz, Daniel M.

doi:10.1136/bjo.2009.168864

Cited by 26 publications

(16 citation statements)

References 69 publications

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“…In one of these studies, it was reported that the thermal environment of the retina is important, and that choroidal circulation modulates the thermal environment of the macula [31]. Studies associating VEGF with intraocular temperature, which have found that VEGF secreted from the retinal pigment epithelium decreased in case of hypothermia, have also been published [32, 33]. …”

Section: Discussionmentioning

confidence: 99%

Seasonal Variation in Japanese Central Serous Chorioretinopathy

Kobayashi¹,

Sato²,

Fukumoto³

et al. 2018

Ophthalmologica

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Purpose: We investigate seasonal variations found in patients with central serous chorioretinopathy (CSC). Methods: We retrospectively investigated 201 CSC patients at the Osaka Medical College Hospital from January 2011 to December 2016 and evaluated their seasonal and monthly distributions. Results: The study population comprised 149 males and 52 females. There were significant seasonal differences in the distribution of patients, with 62 cases in spring, 37 in summer, 59 in autumn, and 43 in winter, and the monthly prevalence of CSC was high in March (n = 24), April (n = 22), and November (n = 23). In addition, seasonal variation of serous retinal detachment (SRD) in the macula could be observed in 12 recurrent CSC cases. Conclusions: The prevalence of CSC development was the highest in spring. In addition, recurrent SRD in the macula showed seasonal variation in some CSC patients. As factors related to CSC, season and temperature might influence the pathophysiology of CSC.

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Section: Discussionmentioning

confidence: 99%

Seasonal Variation in Japanese Central Serous Chorioretinopathy

Kobayashi¹,

Sato²,

Fukumoto³

et al. 2018

Ophthalmologica

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“…Supporting these data, there are also some in vitro and ex vivo experiments showing that exposing isolated retinas or cultured retinal cells to cold temperatures results in reducing NMDA-mediated excitotoxicity [19], reducing secretion of vascular endothelial growth factor (VEGF) [20], and increasing survival against toxicants [21]. Hopefully, these promissing observations would be translated into clinical treatments soon.…”

Section: Introductionmentioning

confidence: 99%

Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures

et al. 2016

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Hypothermia has been proposed as a therapeutic intervention for some retinal conditions, including ischemic insults. Cold exposure elevates expression of cold-shock proteins (CSP), including RNA-binding motif protein 3 (RBM3) and cold inducible RNA-binding protein (CIRP), but their presence in mammalian retina is so far unknown. Here we show the effects of hypothermia on the expression of these CSPs in retina-derived cell lines and in the retina of newborn and adult rats. Two cell lines of retinal origin, R28 and mRPE, were exposed to 32°C for different time periods and CSP expression was measured by qRT-PCR and Western blotting. Neonatal and adult Sprague-Dawley rats were exposed to a cold environment (8°C) and expression of CSPs in their retinas was studied by Western blotting, multiple inmunofluorescence, and confocal microscopy. RBM3 expression was upregulated by cold in both R28 and mRPE cells in a time-dependent fashion. On the other hand, CIRP was upregulated in R28 cells but not in mRPE. In vivo, expression of CSPs was negligible in the retina of newborn and adult rats kept at room temperature (24°C). Exposure to a cold environment elicited a strong expression of both proteins, especially in retinal pigment epithelium cells, photoreceptors, bipolar, amacrine and horizontal cells, Müller cells, and ganglion cells. In conclusion, CSP expression rapidly rises in the mammalian retina following exposure to hypothermia in a cell type-specific pattern. This observation may be at the basis of the molecular mechanism by which hypothermia exerts its therapeutic effects in the retina.

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“…Incubation of human vascular endothelial cells under “hypothermic” conditions (i.e., ≤34°C) reduces their capacity to form tubes in vitro (Coassin et al, 2010; Takeyama et al, 2015). Hypothermia also significantly reduces VEGF secretion and mRNA expression by retinal pigment epithelial cells (Coassin et al, 2010; Takeyama et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Second, angiogenesis is closely linked with muscle repair. Evidence from other tissue types suggests that hypothermia can alter the secretion of vascular endothelial growth factor (VEGF) (Coassin et al, 2010; Takeyama et al, 2015) and angiogenesis (Kuo et al, 2010; Kao et al, 2011). It remains unknown whether hypothermia exerts similar effects in skeletal muscle tissue.…”

Section: Introductionmentioning

confidence: 99%

Effects of Topical Icing on Inflammation, Angiogenesis, Revascularization, and Myofiber Regeneration in Skeletal Muscle Following Contusion Injury

et al. 2017

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Contusion injuries in skeletal muscle commonly occur in contact sport and vehicular and industrial workplace accidents. Icing has traditionally been used to treat such injuries under the premise that it alleviates pain, reduces tissue metabolism, and modifies vascular responses to decrease swelling. Previous research has examined the effects of icing on inflammation and microcirculatory dynamics following muscle injury. However, whether icing influences angiogenesis, collateral vessel growth, or myofiber regeneration remains unknown. We compared the effects of icing vs. a sham treatment on the presence of neutrophils and macrophages; expression of CD34, von Willebrands factor (vWF), vascular endothelial growth factor (VEGF), and nestin; vessel volume; capillary density; and myofiber regeneration in skeletal after muscle contusion injury in rats. Muscle tissue was collected 1, 3, 7, and 28 d after injury. Compared with uninjured rats, muscles in rats that sustained the contusion injury exhibited major necrosis, inflammation, and increased expression of CD34, vWF, VEGF, and nestin. Compared with the sham treatment, icing attenuated and/or delayed neutrophil and macrophage infiltration; the expression of vWF, VEGF, and nestin; and the change in vessel volume within muscle in the first 7 d after injury (P < 0.05). By contrast, icing did not influence capillary density in muscle 28 d after injury (P = 0.59). The percentage of immature myofibers relative to the total number of fibers was greater in the icing group than in the sham group 28 d after injury (P = 0.026), but myofiber cross-sectional area did not differ between groups after 7 d (P = 0.35) and 28 d (P = 0.30). In conclusion, although icing disrupted inflammation and some aspects of angiogenesis/revascularization, these effects did not result in substantial differences in capillary density or muscle growth.

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Hypothermia reduces secretion of vascular endothelial growth factor by cultured retinal pigment epithelial cells

Cited by 26 publications

References 69 publications

Seasonal Variation in Japanese Central Serous Chorioretinopathy

Seasonal Variation in Japanese Central Serous Chorioretinopathy

Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures

Effects of Topical Icing on Inflammation, Angiogenesis, Revascularization, and Myofiber Regeneration in Skeletal Muscle Following Contusion Injury

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