Hypothermic and Low-Temperature Storage of Garlic (Allium sativum L.) for in Vitro Collections

Ivchenko, Tetyana V.; Vitsenya, T. I.; Shevchenko, Nadiia; Bashtan, Natalia O.; Корниенко, С. И.

doi:10.15407/cryo27.02.110

Cited by 4 publications

(2 citation statements)

References 2 publications

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“…The first group contained meristems dehydrated for 120 min with sterile airflow (AD), frozen by plunging into LN on a needle tip, and re-warmed in MS medium, enriched with 12%sucrose. Other groups consisted of meristems incubated for 60 min at 22 °C with the following cryoprotectant solutions: (i) PVS 2 (30% glycerol + 15% ethylene glycol + 15% dimethyl sulfoxide and 0.4 M sucrose [11]), (ii) 88% PVS 3 (44% glycerol + 44% sucrose [8]), (iii) modified PVS 1 (22% glycerol + 13% 1,2-propylene glycol + 13% ethylene glycol + 6% dimethyl sulfoxide and 0.4 M sucrose [14]) and (iv) PVS N (1 M sucrose + 15% glycerol + 14% ethylene glycol [4]). The meristems of all groups after PVSs treatment were cooling 1.8 mL cryovials («Corning», USA) or 50 µl hermetically sealed aluminum pans for differential scanning calorimetry (DSC) and were directly immersed into LN for 1 h. The specimens were re-warmed in water bath at 40 °C.…”

Section: Methodsmentioning

confidence: 99%

Post-Thaw Survival of Meristems from In Vitro Sweet Potato (Ipomoea batatas (L.) Lam.) Plants

Shevchenko

Mozgovska

Bobrova

et al. 2020

The 1st International Electronic Conference on Plant Science

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Cryotherapy of shoot tips can effectively eliminate the sweet potatoes pathogens, such as viruses and phytoplasm, and is impossible without the development of effective cryopreservation techniques. At the same time, cryopreservation allows a long-term germplasm storage. In this study, we developed and compared different methods for sweet potatoes’ meristems treatment for optimized preservation. The meristems of Admiral variety up to 1–2 mm were isolated from in vitro growth plants. In one group the specimens were dehydrated for 120 min with sterile air flow and immersed into liquid nitrogen at a needle tip. The meristems of other groups were dehydrated with plant vitrification solutions (modified PVS 1, PVS 2, 88% PVS 3, and PVS N). The samples were immersed into liquid nitrogen either in 1.8 mL cryovials or 50 µL hermetically sealed aluminum pans for differential scanning calorimetry. It was shown that the survival rates of meristems were 55% after 88% PVS 3 treatment and 83 and 85% after PVS 2 and PVS N exposure. The highest percentage of preserved specimens was found after dehydration with air-flow and modified PVS 1 (89–95%). After cryopreservation in 1.8 mL cryovials the highest post-thaw preservation was noted after pretreatment with modified PVS 1 (60–75%) and the lowest one was observed with 88% PVS 3 (35–40%). Meristems treated with PVS 2 and PVS N provided 45–55% survival rates. After cryopreservation in aluminum pans the highest post-thaw preservation was detected for dehydration with modified PVS 1 (81–84%) and the lowest one was found with 88% PVS 3 (40–51%). Meristems treated with PVS 2 and PVS N revealed of 68–78% post-thaw survival respectively. The meristems cryopreservation method based on dehydration with sterile air flow is of special interest, since no cryoprotectant use is needed.

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Section: Methodsmentioning

confidence: 99%

Post-Thaw Survival of Meristems from In Vitro Sweet Potato (Ipomoea batatas (L.) Lam.) Plants

Shevchenko

Mozgovska

Bobrova

et al. 2020

The 1st International Electronic Conference on Plant Science

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“…To significantly reduce the intensity of metabolic processes (respiration, water loss, wilting, ethylene release) and to slow down the processes of physiological aging of cells in in vitro cultures, to prevent depletion of nutrient media, and to reduce chances of damage to plant material by bacterial infections, one can lower the storage temperature (Bamberg, J. B. et al, 2016, Ivchenko, T. et al, 2017. However, when explants are stored at low above-zero temperatures in temperaturedemanding cultures, excessive formation of reactive oxygen species is observed and they are able to lead to stress and induce mutations because of damage to DNA (Cassells, A.C. & Curry, R.F., 2001).…”

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confidence: 99%

Medium Composition for in Vitro Mid-Term Storage of Solanum Habrochaites Test-Tube Plants

Miroshnichenko,

Ivchenko,

Bashtan

et al. 2024

Vegetab. and Mel. Grow.

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Purpose. To investigate effects of osmotically active substances on the growth and development of isolated microcuttings of the wild tomato species S. habrochaites Knapp & Spooner and to determine an optimal composition of nutrient medium for their mid-term storage in vitro. Methods. General scientific, laboratory, biotechnological, measuring, statistical. Results. Possibilities of subcultivation-free storage of S. habrochaites (line BK-78) microcuttings in Murashige & Skug medium with different concentrations of sucrose (30, 60, 90 and 120 g/L), agar-agar (6, 9, 12 and 15 g/l) and sorbitol (0, 20, 40 and 60 g/L) for 30 (control), 90, 180 and 270 days under standard cultivation conditions were researched. The survivability, height, number of leaves, and root length in regenerants on different modifications of the medium were determined. There was an inversely proportional relationship between the percentage of viable regenerants and the time of in vitro storage without subcultivation on all variants of the medium. The maximum survivability of explants was achieved on the medium supplemented with 60 g/L of sorbitol (100.0 – 66.7% depending on the storage period), the minimum - on the medium containing 120 g/L of sucrose (40.0 – 22.5% ). A significant effect of the medium composition on the plant growth intensity was observed in the variants with 20, 40, or 60 g/L of sorbitol and 90 or 120 g/L of sucrose. Sorbitol-containing media also caused a 2- to 7.5-fold decrease in the mean height of regenerants and a 2.4- to 2.5-fold decrease in the number of leaves compared to the control. The root length of test-tube plants did not depend on the medium composition, except for the variants with 60 g/L of sorbitol and 120 g/L of sucrose. On media with increased agar-agar concentrations, the rate of explants development did not decrease, so these media are unsuitable for mid-term storage of in vitro collections. Conclusions. Among the studied medium modifications, the nutrient medium MS supplemented with 60 g/L of sorbitol turned out to be the most effective formulation for slow growth and mid-term storage of S. habrochaites regenerants, as it allows 66.7% of viable explants to be preserved for 270 days. However, through the lens of hyperhydration of test-tube plants, further optimization of in vitro storage conditions for this species is needed.

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Features of the Growth and Development of Lettuce Plants Sowed Under Hydroponic Growing

Palamarchuk,

Mikhalchuk

2024

Naukovì dopovìdì Nacìonalʹnogo unìversitetu bìoresursìv ì priro

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Research is devoted to the study of the assortment of lettuce seeded under hydroponics conditions. The survival rate of lettuce plants depended on the type and variety, and also differed to a certain extent by the terms of sowing. Plants of the Aficion variety of leaf lettuce (control) had the highest percentage of engraftment - 98.1%, which is 0.6 and 3.7% more compared to other variants of the experiment. Plants of the Raphael variety had a higher survival rate in romaine lettuce – 97.9%, which is 1.4% more than the control variant. Among the varieties of romaine lettuce, plants of the Maximus variety (control) had a higher height – 16.1 cm, which is 3.8 cm more than the Raphael variety. Comparing leaf lettuce plants, the highest height was noted in the Aficion variety (control) – 13.3 cm, which is 0.9 and 1.8 cm more than the other tested variants. In romaine lettuce, a larger diameter of the rosette was noted in the Maximus variety – 25.2 cm, which is 9.1 cm more than the Raphael variety. Among the leaf-type lettuce varieties, the largest diameter of the rosette of leaves was characterized by plants of the Aficion variety (Batavia type) – 28.3 cm, which is 4.0 and 7.9 cm more than the other studied variants of the experiment. In romaine lettuce, plants of the Maximus variety were characterized by a greater mass of the above-ground part – 210 g, which is 80.0 g more than the Raphael variety. Among the varieties of leaf lettuce, the Kirinia variety had the largest mass – 200 g, which is 60.0 more than the control variant. Mr. Maximus variety romaine lettuce had the highest yield – 9.3 kg/m2, and in terms of rows – 37.0 kg/m2, which is 14.1 kg/m2 more than the Raphael variety. Among the leafy types of lettuce, plants of the Kirinia variety were characterized by a higher yield – 8.8 kg/m2, in terms of rows – 35.2 kg/m2, where the increase compared to the control was 10.6 kg/m2. The yield of different types of lettuce ranged from 158.4 t/ha to 369.6 t/ha, taking into account the rows of racks. The highest yield, taking into account the tiering of racks, was obtained by growing Maximus (romaine type) – 369.6 t/ha and Kirinia (oak type) – 352.0 t/ha with an increase of 42.9 t/ha compared to the control variant.

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Hypothermic and Low-Temperature Storage of Garlic (Allium sativum L.) for in Vitro Collections

Cited by 4 publications

References 2 publications

Post-Thaw Survival of Meristems from In Vitro Sweet Potato (Ipomoea batatas (L.) Lam.) Plants

Post-Thaw Survival of Meristems from In Vitro Sweet Potato (Ipomoea batatas (L.) Lam.) Plants

Medium Composition for in Vitro Mid-Term Storage of Solanum Habrochaites Test-Tube Plants

Features of the Growth and Development of Lettuce Plants Sowed Under Hydroponic Growing

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