Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid

Nguyen, Nhien Thi; Hirata, Maki; Tanihara, Fuminori; Hirano, Takayuki; Le, Quynh Anh; Nii, Masahiro; Otoi, Takeshige

doi:10.1111/rda.13417

Cited by 5 publications

(9 citation statements)

References 25 publications

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“…Moreover, Pomar et al (2004) reported that in vivo‐produced porcine embryos at the morula and blastocyst stages have a feasible developmental quality and viability when stored at 25°C. In a previous study, we also confirmed the development of porcine zygotes up to the blastocyst stage when stored at 25°C for 24 h (Thi Nguyen et al, 2019). In the present study, we observed that the blastocyst formation rate in porcine zygotes stored in 100% FBS supplemented with 50 μM CGA was similar to that of zygotes stored in a chemically defined storage solution.…”

Section: Discussionsupporting

confidence: 84%

Short‐term preservation of porcine zygotes at ambient temperature using a chemically defined medium

Lin

Takebayashi

et al. 2022

Animal Science Journal

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We aimed to develop a simple method for the short-term preservation of in vitro-produced porcine zygotes at 25 C for up to 2 days. Firstly, we evaluated the efficiency of three storage solutions to preserve porcine zygotes at 25 C for 24 h. Two of these were commercially available defined media for cell storage (Cell-W and Cell-S), and the third was fetal bovine serum (FBS). Thereafter, we examined the effects of storing the zygotes in the Cell-W solution for 24-72 h at 25 C. The Cell-W solution was the most efficient for 24 h storage of porcine zygotes at 25 C, with no apparent effects on blastocyst quality. However, short-term storage of porcine zygotes for 24 h reduced the blastocyst formation rate compared with the fresh control group. As storage duration increased from 24 to 48 or 72 h, blastocyst formation rates were significantly decreased from 11.3% to 4.4% and 0%, respectively. In conclusion, during zygote storage, the developmental competence to the blastocyst stage decreased with time. Storage of zygotes in chemically defined media did not affect blastocyst quality, but the storage in 100% serum had an adverse effect on developing embryos causing apoptosis.

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Section: Discussionsupporting

confidence: 84%

Short‐term preservation of porcine zygotes at ambient temperature using a chemically defined medium

Lin

Takebayashi

et al. 2022

Animal Science Journal

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“…In addition to the medical field, CGA acts as a drug delivery material ( 142 , 143 ), participates in developing new drugs ( 144 , 145 ), etc. In animal husbandry, CGA is added to livestock feed to regulate intestinal flora, and increase the preservation period of animal sperm ( 146 , 147 ), etc. CGA is often involved in cosmetics and daily chemical products due to of its good anti-oxidant and anti-bacterial properties ( 148 , 149 ).…”

Section: Discussionmentioning

confidence: 99%

The Biological Activity Mechanism of Chlorogenic Acid and Its Applications in Food Industry: A Review

et al. 2022

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Chlorogenic acid (CGA), also known as coffee tannic acid and 3-caffeoylquinic acid, is a water-soluble polyphenolic phenylacrylate compound produced by plants through the shikimic acid pathway during aerobic respiration. CGA is widely found in higher dicotyledonous plants, ferns, and many Chinese medicine plants, which enjoy the reputation of “plant gold.” We have summarized the biological activities of CGA, which are mainly shown as anti-oxidant, liver and kidney protection, anti-bacterial, anti-tumor, regulation of glucose metabolism and lipid metabolism, anti-inflammatory, protection of the nervous system, and action on blood vessels. We further determined the main applications of CGA in the food industry, including food additives, food storage, food composition modification, food packaging materials, functional food materials, and prebiotics. With a view to the theoretical improvement of CGA, biological activity mechanism, and subsequent development and utilization provide reference and scientific basis.

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“…Supplementation of antioxidants in the medium used for short‐term storage has been shown to improve embryo survival (Thi Nguyen et al., 2019). In the present study, supplementation of curcumin into a chemically defined solution improved the development of stored embryos.…”

Section: Discussionmentioning

confidence: 99%

“…To date, the potential applications for liquid storage of porcine embryos have been limited. In a previous study, we reported the storage of porcine zygotes using 100% serum enhanced blastocyst formation after short-term storage for 24 hr at 25°C, but the quality of the resulting embryos remained to be improved (Thi Nguyen et al, 2019). In addition, several types of serum-containing or bovine serum albumin (BSA)-containing media have been shown to be effective for short-term storage of porcine embryos (Pomar et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Exposure to environmental stress such as hypothermic storage, mainly due to a severe imbalance in the levels of reactive oxygen species (ROS) and antioxidant defence mechanisms in the culture medium, may adversely affect the developmental capacity of the embryos (Halliwell, 2014; de Lamirande et al., 1997). Supplementation with antioxidants as external protection against oxidative stress may be an effective strategy for embryo storage (Thi Nguyen et al., 2019). Curcumin has been demonstrated to reduce oxidative stress by scavenging free radicals and preventing ROS generation (Tapia et al., 2014).…”

Section: Introductionmentioning

confidence: 99%

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Viability and developmental potential of porcine blastocysts preserved for short term in a chemically defined medium at ambient temperature

Lin

Takebayashi

et al. 2022

Reprod Domestic Animals

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This study developed an efficient method for liquid storage of in vitro‐derived porcine blastocysts at ambient temperature for 24 hr. We evaluated the effects of new chemically defined media (cell wash and preservation solution, Cellstor®‐W [Cell‐W] and cell suspension and preservation solution, Cellstor®‐S [Cell‐S]) for short‐term storage. In the first experiment, in vitro‐derived blastocyst were stored at 25ºC for 24 hr in Cell‐W solution, Cell‐S solution and pig embryo culture (PBM) medium. There were no differences in the rates of survival and development of stored blastocysts between the Cell‐S and Cell‐W solutions, but the total cell number of embryos that survived after storage in Cell‐S solution was significantly higher (p < .05) than that in the Cell‐W solution. In the second experiment, Cell‐S solution was used to store the in vitro‐derived blastocysts at 20°C, 25°C and 30°C. Storage at 20°C resulted in a significant decrease in the rates of survival and development of stored blastocysts compared to storage at 25°C or 30°C. No differences in survival and development rates were observed between storage at 25°C and 30°C, but the damage to the embryo quality after storage and culture was significantly lower at 25°C than at 30°C. In the third experiment, Cell‐S solution was supplemented with β‐mercaptoethanol and curcumin, either alone or in combination, as antioxidant agents. Although the supplementation with curcumin did not improve survival, it significantly increased the development rate of stored blastocysts compared with the control blastocysts stored without antioxidants. In conclusion, when porcine blastocysts were stored at 25°C for 24 hr, a Cell‐S solution may be effective for maintaining the survival and development of in vitro embryos.

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Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid

Cited by 5 publications

References 25 publications

Short‐term preservation of porcine zygotes at ambient temperature using a chemically defined medium

Short‐term preservation of porcine zygotes at ambient temperature using a chemically defined medium

The Biological Activity Mechanism of Chlorogenic Acid and Its Applications in Food Industry: A Review

Viability and developmental potential of porcine blastocysts preserved for short term in a chemically defined medium at ambient temperature

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