Hypothesis: Refractory Animias, Preleukimic Conditions, and Fetal Erythropoiesis

Rochant, H; Dreyfus, B; Bouguerra, M.; Tonthat, H

doi:10.1182/blood.v39.5.721.721

Cited by 54 publications

(4 citation statements)

References 49 publications

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“…Altered expression pattern for the I/i antigens have often been observed during oncogenetic processes as well as in benign haemopoietic disorders [ 43 ] Poly-N-acetyllactosaminyl, I (branched structure) and i (linear structure) structures, are important carbohydrate antigens and are presumed to have essential roles in the process of cellular recognition, differentiation [ 44 ], malignant transformation and cancer metastasis, they are also expressed in carcinoma cells in several tissues and organs [ 45 ]. The i/I transition in cell differentiation is determined by the transcription factor CCAAT/enhancer binding protein alpha (C/EBPalpha), which enhances transcription of the IGnTC gene, consequently leading to formation of the I antigen.…”

Section: Discussionmentioning

confidence: 99%

Cell fusion in tumor progression: the isolation of cell fusion products by physical methods

et al. 2011

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BackgroundCell fusion induced by polyethylene glycol (PEG) is an efficient but poorly controlled procedure for obtaining somatic cell hybrids used in gene mapping, monoclonal antibody production, and tumour immunotherapy. Genetic selection techniques and fluorescent cell sorting are usually employed to isolate cell fusion products, but both procedures have several drawbacks.ResultsHere we describe a simple improvement in PEG-mediated cell fusion that was obtained by modifying the standard single-step procedure. We found that the use of two PEG undertreatments obtains a better yield of cell fusion products than the standard method, and most of these products are bi- or trinucleated polykaryocytes. Fusion rate was quantified using fluorescent cell staining microscopy. We used this improved cell fusion and cell isolation method to compare giant cells obtained in vitro and giant cells obtained in vivo from patients with Hodgkin's disease and erythroleukemia.ConclusionsIn the present study we show how to improve PEG-mediated cell fusion and that cell separation by velocity sedimentation offers a simple alternative for the efficient purification of cell fusion products and to investigate giant cell formation in tumor development.

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Section: Discussionmentioning

confidence: 99%

Cell fusion in tumor progression: the isolation of cell fusion products by physical methods

et al. 2011

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“…Cases I and 3 having marrow hyperplasia. As currently defined (Linman & Saarni, 1974), preleukaemic states do not show more than a slight increase in blast cells and the reported cases of refractory anaemia with excess of myeloblasts (Rochant et al, 1972) have had increased cellularity. Our cases represent a reasonably homogeneous group and present essentially with symptoms and signs related to a generalized marrow failure without evidence of infiltrative lesions.…”

Section: Discussionmentioning

confidence: 86%

Hypoplastic Acute Myelogenous Leukaemia

et al. 1975

Br J Haematol

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The clinical course, diagnosis and management of nine cases of hypoplastic acute myelogenous leukaemia are described. Such cases may follow a slowly progressive course and should not receive anti-leukaemic chemotherapy unless the disease is advancing rapidly or unless some specific complication develops. If chemotherapy has to be given, usually because of severe and recurrent infections, then prompt and prolonged remission of disease may occur.

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“…Abnormal iron metabolism may result in iron-deposited marrow macrophages, ringed sideroblasts containing iron-loaded mitochondria or ferritin aggregates, and increased erythrocyte ferritin concentration [93,94]. Erythrocytes show a wide variation of metabolic abnormalities [93-951, which may be associated with the reappearance of the Hb F [96], changes in membrane antigens [W-1001 and imbalance in globin chain synthesis [101, 1021. Granulocytes may show reduced myeloperoxidase [103-1051 and abnormal esterase cytochemistry [106]. Subtle defects in neutrophilic functions have been reported, including defective chemotaxis, adhesion, phagocytosis and bactericidal activity [103-1071.…”

Section: Cellular Dysfunctionmentioning

confidence: 99%

Biology of myelodysplastic syndromes

Yoshida

1987

The International Journal of Cell Cloning

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Myelodysplastic syndromes (MDS) represent a diverse spectrum of disorders ranging from refractory anemia to a preleukemic state. Peripheral cytopenias, cellular marrow, dysplasias and dysfunctions of myeloid and lymphoid cells constitute hematological hallmarks, and are caused by ineffective hemopoiesis. Investigations of cell cultures and cellular functions indicate that the disease originates in a stem cell pluripotent to all myeloid cells and possibly lymphoid cells as well. The disease commonly runs a chronic indolent course, often terminating in acute leukemia or nonleukemic death, notably infections and/or hemorrhage due to cytopenias and cellular dysfunctions. Clonal expansion or clonal evolution appears to be related to the disease progression with a greater degree of malignancy. However, the initial sequence of events causing damage to stem cells is still undefined.

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Hypothesis: Refractory Animias, Preleukimic Conditions, and Fetal Erythropoiesis

Cited by 54 publications

References 49 publications

Cell fusion in tumor progression: the isolation of cell fusion products by physical methods

Cell fusion in tumor progression: the isolation of cell fusion products by physical methods

Hypoplastic Acute Myelogenous Leukaemia

Biology of myelodysplastic syndromes

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