Hypothyroidism in Adult Male Rats Alters Posttranscriptional Mechanisms of Luteinizing Hormone Biosynthesis

Romano, Renata Marino; Bargi‐Souza, Paula; Brunetto, Erika Lia; Goulart-Silva, Francemilson; Avellar, Maria Christina W.; Oliveira, Cláudio Alvarenga de; Nunes, Maria Tereza

doi:10.1089/thy.2011.0514

Cited by 21 publications

(21 citation statements)

References 32 publications

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“…Thirty minutes after treatment with T 3 , the poly(A) tail length of Tshb mRNA of Tx rats was reduced to values similar to those observed in the poly(A) tail length of euthyroid rats and was positively correlated with a marked reduction in the content of Tshb in polysome fractions indicating that T 3 rapidly reduced mRNA translation and TSH protein synthesis (GoulartSilva et al 2011, Bargi-Souza et al 2013.…”

Section: Rapid and Negative Posttranscriptional Regulation Of Cga Andmentioning

confidence: 91%

“…Using Tx rats, we observed that in hypothyroidism, the poly(A) tail length of Tshb mRNA is increased, followed by a significant rise in the translational rate of Tshb, besides the expected increase in the transcriptional process (Bargi-Souza et al 2013). Thirty minutes after treatment with T 3 , the poly(A) tail length of Tshb mRNA of Tx rats was reduced to values similar to those observed in the poly(A) tail length of euthyroid rats and was positively correlated with a marked reduction in the content of Tshb in polysome fractions indicating that T 3 rapidly reduced mRNA translation and TSH protein synthesis (GoulartSilva et al 2011, Bargi-Souza et al 2013.…”

Section: Rapid and Negative Posttranscriptional Regulation Of Cga Andmentioning

confidence: 91%

“…Our recent studies indicate that alterations in the HPT axis strongly affect the hypothalamus-pituitarygonad axis in rats, which might explain some reproductive disorders observed under hypothyroid and hyperthyroid conditions (Romano et al 2013).…”

Section: Negative Transcriptional Regulation Of Cga and Tshb Genes Bymentioning

confidence: 96%

“…On the other hand, studies performed in hyperthyroid rodents and in T 3 -treated TtT97 cells, a thyrotrophic tumor-transplantable cell line, have shown a marked increase in Gh mRNA content and a significant reduction in Cga and Tshb contents (Shupnik et al 1985, Chin et al 1993, Volpato & Nunes 1994, Shupnik 2000, Bargi-Souza et al 2013.…”

Section: Nuclear Thyroid Receptors and The Gene Expression Regulationmentioning

confidence: 99%

“…Considering the positive effects of T 3 on the posttranscriptional steps of Gh synthesis, our group has investigated whether T 3 could trigger rapid effects on Tshb mRNA content and poly(A) tail length (Goulart- Silva et al 2011, Bargi-Souza et al 2013.…”

Section: Rapid and Negative Posttranscriptional Regulation Of Cga Andmentioning

confidence: 99%

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Novel aspects of T3 actions on GH and TSH synthesis and secretion: physiological implications

Bargi‐Souza

Goulart-Silva

Nunes

2017

Journal of Molecular Endocrinology

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Thyroid hormones (THs) classically regulate the gene expression by transcriptional mechanisms. In pituitary, the encoding genes for growth hormone (GH) and thyroid-stimulating hormone (TSH) are examples of genes regulated by triiodothyronine (T3) in a positive and negative way, respectively. Recent studies have shown a rapid adjustment of GH and TSH synthesis/secretion induced by T3posttranscriptional actions. In somatotrophs, T3promotes an increase inGhmRNA content, poly(A) tail length and binding to the ribosome, associated with a rearrangement of actin cytoskeleton. In thyrotrophs, T3reducesTshbmRNA content, poly(A) tail length and its association with the ribosome. In parallel, it promotes a redistribution of TSH secretory granules to more distal regions of the cell periphery, indicating a rapid effect of T3inhibition of TSH secretion. T3was shown to affect the content of tubulin and the polymerization of actin and tubulin cytoskeletons in the whole anterior pituitary gland, and to increase intracellular alpha (CGA) content. This review summarizes genomic and non-genomic/posttranscriptional actions of TH on the regulation of several steps of GH and TSH synthesis and secretion. These distinct mechanisms induced by T3can occur simultaneously, even though non-genomic effects are promptly elicited and precede the genomic actions, coexisting in a functional network within the cells.

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Section: Rapid and Negative Posttranscriptional Regulation Of Cga Andmentioning

confidence: 91%

Section: Rapid and Negative Posttranscriptional Regulation Of Cga Andmentioning

confidence: 91%

Section: Negative Transcriptional Regulation Of Cga and Tshb Genes Bymentioning

confidence: 96%

Section: Nuclear Thyroid Receptors and The Gene Expression Regulationmentioning

confidence: 99%

Section: Rapid and Negative Posttranscriptional Regulation Of Cga Andmentioning

confidence: 99%

See 3 more Smart Citations

Novel aspects of T3 actions on GH and TSH synthesis and secretion: physiological implications

Bargi‐Souza

Goulart-Silva

Nunes

2017

Journal of Molecular Endocrinology

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Multi-omics Investigations in Endocrine Systems and Their Clinical Implications

Peliciari-Garcia,

de Barros,

Secio-Silva

et al. 2024

Advances in Experimental Medicine and Biology

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New insights for male infertility revealed by alterations in spermatic function and differential testicular expression of thyroid-related genes

et al. 2016

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The impact of thyroid hormone (TH) disorders on male reproductive biology has been a controversial issue for many years. Recently, we reported that hypothyroid male rats have a disruption of the seminiferous epithelium, which may compromise spermatogenesis. To improve the understanding of the reproductive pathogenesis of hypothyroidism and hyperthyroidism, male Wistar rats that developed these dysfunctions in adulthood were used as an experimental model. We evaluated the sperm production, reserves, transit time, morphology, and functionality (acrosome integrity, plasma membrane integrity, and mitochondrial activity), and the testicular expression of the TH receptors (Thra1 and Thra2, Thrb1, and Thrb2), deiodinases (Dio2 and Dio3), and the Mct8 transporter (Slc16a2) were assessed by reverse transcription followed by real-time quantitative PCR (RT-qPCR). The results were evaluated statistically by ANOVA and Tukey HSD test (P < 0.05). Hypothyroidism decreased the total and daily sperm productions and increased the sperm transit time through the epididymis, while the sperm functionality was reduced in both thyroid dysfunctions. Regarding the modulation of gene expression in the testis, hypothyroidism increased the expression of Thra1 and decreased the expression of Dio3, and hyperthyroidism increased the expression of Slc16a2. The observed alterations in spermatic production and function and in the expression of the TH receptor, deiodinase, and the TH transporter are suggestive of TH participation in spermatogenesis in adulthood.

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Hypothyroidism in Adult Male Rats Alters Posttranscriptional Mechanisms of Luteinizing Hormone Biosynthesis

Cited by 21 publications

References 32 publications

Novel aspects of T3 actions on GH and TSH synthesis and secretion: physiological implications

Novel aspects of T3 actions on GH and TSH synthesis and secretion: physiological implications

Multi-omics Investigations in Endocrine Systems and Their Clinical Implications

New insights for male infertility revealed by alterations in spermatic function and differential testicular expression of thyroid-related genes

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