Hypouricemia effects of corn silk flavonoids in a mouse model of potassium oxonated‐induced hyperuricemia

Yuan, Liyan; Bao, Zhijie; Ma, Tiecheng; Lin, Songyi

doi:10.1111/jfbc.13856

Cited by 23 publications

(17 citation statements)

References 38 publications

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“…This suggests that hexoses in positions 3 and 4′ give a different pharmacological profile to kaempferol-3,4′-di- O -β-glucoside, since the treatment was remarkably effective intraperitoneally and one of the mechanisms involved was uricosuric. Previous studies [ 27 ] have suggested that the presence of hydroxyl groups at positions C3, C5, C7 and C-4′ of the chemical structure of flavonoids are important for XO-inhibiting and antihyperuricaemic activity. This theory may explain why kaempferol-3,4′-di- O -β-diglucoside showed no effect on hepatic xanthine oxidase activity.…”

Section: Resultsmentioning

confidence: 99%

Chemical Analysis of Eruca sativa Ethanolic Extract and Its Effects on Hyperuricaemia

et al. 2022

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In vivo assays and chemical analyses were performed on the ethanolic extract from leaves of Eruca sativa. UHPLC-ESI-QTOF analysis confirmed the presence of glucosinolates and flavonol glucosides. The major flavonoid of the ethanolic extract, kaempferol-3,4′-di-O-β-glucoside, was isolated, a HPLC-DAD method developed and validated to quantify its content in the extract. In vivo experiments were carried out on Wistar rats with hyperuricaemia induced by potassium oxonate and uric acid. A hypouricaemic effect was observed in hyperuricaemic Wistar rats treated with ethanolic extract at dose of 125 mg/kg and kaempferol-3,4′-di-O-β-glucoside at dose of 10 mg/kg. The main anti-hyperuricaemic mechanism observed in the extract was uricosuric. Kaempferol-3,4′-β-O-diglucoside was identified as an important component responsible for the total activity of the ethanolic extract and was considered as a good chemical and biological marker of the ethanolic extract of E. sativa. The obtained results indicated the potential of E. sativa in the treatment of hyperuricaemia and its comorbidities.

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Section: Resultsmentioning

confidence: 99%

Chemical Analysis of Eruca sativa Ethanolic Extract and Its Effects on Hyperuricaemia

et al. 2022

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“…Also, compounds including naringenin, kaempferol, apigenin, luteolin, aesculin, orientin, hyperoside, diosmin, etc. were identified in the ethanolic extract of corn silk by hybrid-quadrupole-TOF LC/MS/MS, which may be responsible for its hypouricemic effect . A plethora of research studies illustrated the protective action of various phytocompounds that are identified by UPLC-MS in kidney-related disorders.…”

Section: Discussionmentioning

confidence: 99%

Metabolite Profiling and Nephroprotective Potential of the Zea mays L. Silk Extract against Diclofenac-Induced Nephrotoxicity in Wistar Rats

et al. 2022

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The lack of sufficient scientific evidence prompted the analytical investigation of nephroprotective potential of the silk extract of Zea mays L., which is traditionally and ethnomedicinally used for various disorders including kidney dysfunction. The present study was conducted to investigate the phytochemical analysis and demonstrate the nephroprotective potential of the methanolic silk extract of Z. mays L. using a rodent model. High-performance thin-layer chromatography (HPTLC) analysis was carried out to standardize the methanolic silk extract of Z. mays (ZME) using naringenin as a marker. The metabolite profiling of the ZME was carried out using ultrahigh-performance liquid chromatography mass spectrometry (UPLC-MS) on a monolithic capillary silica-based C18 column to identify bioactive compounds and for confirmation of the identified markers. Furthermore, for acute toxicity study, a single dose (2000 mg/kg bw) of the ZME was administered orally to Wistar rats. Also, nephrotoxicity was induced in Wistar rats by injecting diclofenac (DC) (50 mg/kg, bw, i.p.) at a single dose. The efficacy of the ZME as a nephroprotective agent was then evaluated at doses of 100, 200, and 400 mg/kg/day, bw, p.o. Furthermore, the kidney, liver, antioxidant, inflammatory, and apoptotic biochemical markers and histopathological and immunohistochemical alterations (caspase-3 and reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-4 (NOX-4)) were evaluated. Phytochemical analysis by HPTLC and UPLC-MS revealed the presence of naringenin, vanillic acid, ferulic acid, gallic acid (GA), ellagic acid, quercetin, and morin, along with other bioactive constituents exhibiting multiple pharmacological properties. The acute toxicity study of the ZME showed no mortality or any clinical signs of toxicity through all the 14 days of the toxicity study at a dose of 2000 mg/kg. Also, administration of DC caused a significant elevation (P < 0.001) in kidney biochemical parameters and also caused oxidative, inflammatory, and apoptotic stress. Furthermore, DC also caused histopathological and immunohistochemical changes. Pretreatment with the ZME attenuated the elevated biochemical markers significantly at medium and high doses along with improvement in histopathological and immunohistochemical damages and showing comparable results to those of α-ketoanalogue. The present study verifies the traditional claims of Z. mays silk alleviating various kidney and related disorders by concluding the nephroprotective potential of the ZME. The nephroprotective activity of the ZME is attributed to the phytoconstituents present, acting as potent restoring antioxidants and preventing inflammatory and apoptotic cellular damages in rats. Thus, it holds promising potential in the management of nephrotoxicity.

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“…Modern research shows that corn silk contains sugars, flavonoids, mineral elements, volatile oils, alkaloids, amino acids, and other chemical components that have anti-oxidant [47], antibacterial [48], antitumor [49], blood sugar-lowering [50], and other pharmacological effects. A recent study found that corn silk can reduce serum uric acid levels by 26.69% (p < 0.05) and serum xanthine oxidase (XO) activity by 11.29% (p < 0.05) [51]. In addition, a study found that corn silk extract suppressed plasma uric acid in high salt-fed rats (p < 0.05) [13].…”

Section: Discussionmentioning

confidence: 99%

Treatment of Gout with TCM Using Turmeric and Corn Silk: A Concise Review Article and Pharmacology Network Analysis

Zhang

Jiang

Zhao

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Objective. This work aimed to study the compounds, targets, and pathways of turmeric and corn silk for gout and to explore the mechanism of “the same disease with different treatments” based on network pharmacology and molecular docking. Methods. We used the TCMSP, PubChem, and SEA databases to screen the compounds and targets of turmeric and corn silk, gout-related proteins through TTD, Drugbank, DisGeNET, GeneCards, OMIM, and PharmGkb, and used Cytoscape to construct a “compound-target-disease” network. Then, we constructed a protein-protein interaction network (PPI) and used Metascape to perform GO and KEGG analysis. Finally, molecular docking (SYBYL) was used to verify the degree of binding between key targets and compounds. Results. We found bisacumol, campesterol, and stigmasterol to be the main turmeric compounds that exerted a marked effect on gout treatment by targeting protein processing in the endoplasmic reticulum through the HSPA1B, HSP90AB1, and STUB1 proteins. The main corn silk compound, Mandenol, treated gout by targeting the Hippo signaling pathway through the CTNNB1, YWHAG, and YWHAZ proteins. Conclusion. Turmeric and corn silk can treat the same disease, gout, through different pathways and targets. The scientific connotation of “same disease with different treatments” can be preliminarily clarified by analyzing targets and pathways.

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Hypouricemia effects of corn silk flavonoids in a mouse model of potassium oxonated‐induced hyperuricemia

Cited by 23 publications

References 38 publications

Chemical Analysis of Eruca sativa Ethanolic Extract and Its Effects on Hyperuricaemia

Chemical Analysis of Eruca sativa Ethanolic Extract and Its Effects on Hyperuricaemia

Metabolite Profiling and Nephroprotective Potential of the Zea mays L. Silk Extract against Diclofenac-Induced Nephrotoxicity in Wistar Rats

Treatment of Gout with TCM Using Turmeric and Corn Silk: A Concise Review Article and Pharmacology Network Analysis

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