Hypouricemic effect of 2,4-dihydroxybenzoic acid methyl ester in hyperuricemic mice through inhibiting XOD and down-regulating URAT1

Yong, Tianqiao; Liang, Danling; Xiao, Chun; Huang, Longhua; Chen, Shaodan; Xie, Yizhen; Gao, Xiong; Wu, Qingping; Hu, Huiping; Li, Xiangmin; Liu, Yuancao; Cai, Manjun

doi:10.1016/j.biopha.2022.113303

Cited by 9 publications

(2 citation statements)

References 46 publications

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“…In addition, Cr and BUN are two important indicators of renal function, and Cr and BUN have been shown to correlate with glomerular filtration rate and kidney function. 30 The results showed that all groups of YGG significantly reduced the expression levels of serum Cr and UN in rats compared with the model group. This finding was confirmed in histopathological studies of the kidney.…”

Section: Discussionmentioning

confidence: 92%

Study on the Underlying Mechanism of Yinhua Gout Granules in the Treatment of Gouty Arthritis by Integrating Transcriptomics and Network Pharmacology

Fan,

Zhai,

Zhang

et al. 2024

DDDT

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Purpose Yinhua Gout Granules (YGG) is a traditional Chinese medicine preparation with a variety of pharmacological effects, and its clinical efficacy in the treatment of gouty arthritis (GA) has been fully confirmed. However, the pharmacodynamic basis of YGG and its anti-inflammatory mechanism of action in GA are unknown. The objective of this study was to identify the active components and molecular mechanisms of YGG in the treatment of GA. Methods Ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) and network pharmacology were used to identify and predict the potential active ingredients and related signaling pathways. Then, we revealed the anti-GA effects of YGG based on pharmacodynamic experiments in GA rats. Finally, we integrated transcriptomics and network pharmacology to elucidate the potential mechanism of action and verified the putative mechanism by molecular docking, immunohistochemical (IHC) and Western blot. Results We have identified 10 major active components of YGG that may have anti-GA effects, such as ferulic acid, rutin, luteolin, etc. Using molecular docking, we found that 10 major compounds could bind well to TNF, PTGS2, IL-6, IL1β, NOS2 and PTGS1, and the binding energies were all less than −5 kcal/mol. Animal studies have shown that YGG can improve joint inflammation and inflammatory cell infiltration, reduce serum UA, BUN and Cr levels ( p< 0.01), and decrease IL-1β, IL-6, TNF-α, COX-2 and PGE2 levels in synovial tissue ( p< 0.01), which are associated with the pathogenesis of GA. IHC and Western blot results showed that YGG could regulate TLR4/MYD88/NF-κB pathway to inhibit the inflammatory response induced by GA. Conclusion This study found that YGG could not only improve the disease of GA by inhibiting the production of UA in the body, but also target the regulation of TLR4/MYD88/NF-κB signaling pathway through a variety of active components to achieve effective therapeutic effects on GA.

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Section: Discussionmentioning

confidence: 92%

Study on the Underlying Mechanism of Yinhua Gout Granules in the Treatment of Gouty Arthritis by Integrating Transcriptomics and Network Pharmacology

Fan,

Zhai,

Zhang

et al. 2024

DDDT

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“…Anthocyanin. Increase insulin secretion of pancreatic β-cells syringic acid Jan et al (2022) Frontiers in Pharmacology frontiersin.org expression (Leyva-Jimenez et al, 2020), however, isoquercetin inhibited the release of pro-inflammatory cytokines (Yu et al, 2021). Besides, different activities of constituents may owned the similar mechanisms.…”

Section: Figurementioning

confidence: 99%

Comprehensive overview of different medicinal parts from Morus alba L.: chemical compositions and pharmacological activities

Wang,

Ai,

et al. 2024

Front. Pharmacol.

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Morus alba L., a common traditional Chinese medicine (TCM) with a centuries-old medicinal history, owned various medicinal parts like Mori folium, Mori ramulus, Mori cortex and Mori fructus. Different medical parts exhibit distinct modern pharmacological effects. Mori folium exhibited analgesic, anti-inflammatory, hypoglycemic action and lipid-regulation effects. Mori ramulus owned anti-bacterial, anti-asthmatic and diuretic activities. Mori cortex showed counteraction action of pain, inflammatory, bacterial, and platelet aggregation. Mori fructus could decompose fat, lower blood lipids and prevent vascular sclerosis. The main chemical components in Morus alba L. covered flavonoids, phenolic compounds, alkaloids, and amino acids. This article comprehensively analyzed the recent literature related to chemical components and pharmacological actions of M. alba L., summarizing 198 of ingredients and described the modern activities of different extracts and the bioactive constituents in the four parts from M. alba L. These results fully demonstrated the medicinal value of M. alba L., provided valuable references for further comprehensive development, and layed the foundation for the utilization of M. alba L.

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Friedericia Chica, a Medicinal Plant from the Amazon Region, is Repellent Against Aedes Aegypti: In Vivo and Molecular Docking Evidence

Miorando,

Cristina Maccagnan,

Dalla Vecchia

et al. 2024

Chemistry & Biodiversity

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Fridericia chica is widely distributed in Brazil, where it is commonly known as crajiru or pariri in several regions. Despite its popular use for treating inflammations and as an insect repellent, there has been limited assessment of its chemical and biological properties, including its bioinsecticide activities. In this study, we conducted phytochemical analyses and investigated the larvicidal and repellent effects of F. chica against the mosquito Aedes aegypti. The F. chica (HEFc) hydroalcoholic extract was partitioned using column chromatography, and subfractions were analyzed using chromatographic and spectroscopic analyses (ESI‐IT‐MSn and NMR). In addition, HEFc was evaluated for its larvicidal and repellent activities. Phytochemical analyses revealed the presence of 17 constituents, including 2,4‐dihydroxybenzoic and p‐coumaric acids, along with umbelliferone, acetovanilone, myricetin‐3‐O‐glucuronide, and cis‐isorhapontigenin, which are reported for the first time in this species. Although no larvicidal effect was observed at the doses tested, the HEFc exhibited promising repellent effects against A. aegypti, which aligns with its ethnopharmacological potential. In addition, molecular docking studies demonstrated that the compounds of HEFc interacted efficiently with insect odorant binding proteins (OBPs), providing repellent effects. Consistent with the chemical profile and in silico studies, preparations of F. chica have considerable repellent potential.

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Hypouricemic effect of 2,4-dihydroxybenzoic acid methyl ester in hyperuricemic mice through inhibiting XOD and down-regulating URAT1

Cited by 9 publications

References 46 publications

Study on the Underlying Mechanism of Yinhua Gout Granules in the Treatment of Gouty Arthritis by Integrating Transcriptomics and Network Pharmacology

Study on the Underlying Mechanism of Yinhua Gout Granules in the Treatment of Gouty Arthritis by Integrating Transcriptomics and Network Pharmacology

Comprehensive overview of different medicinal parts from Morus alba L.: chemical compositions and pharmacological activities

Friedericia Chica, a Medicinal Plant from the Amazon Region, is Repellent Against Aedes Aegypti: In Vivo and Molecular Docking Evidence

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