Hypovirulence in chestnut blight fungus,<i>Cryphonectria parasitica</i>, in Slovakia

Adamčíková, Katarína; Ondrušková, Emília; Kobza, M.

doi:10.1080/09583157.2019.1608509

Cited by 3 publications

(5 citation statements)

References 43 publications

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“…This change was most apparent following the re‐isolation of virus‐infected isolates from lesions formed on the seedlings, and the same isolates tended to form fewer stromata, were associated with less epicormic growth in the infected plant material and produced smaller lesions. It has previously been reported that mycovirus infection (hypovirulence) in C. parasitica is associated with loss of colony pigmentation and reduced sporulation . However, it was notable in our study that the virus‐infected UK isolates did not have a white colony phenotype and slower growth on initial screening, although low levels of CHV‐1 could be detected (original nanograms per microlitre detections of 9.9 for L‐6 and 9.2 for Db‐1), but only became apparent on re‐isolation from inoculated host material when the virus concentration was also found to have increased (respective concentrations of 31.5 ± 5.7 and 30.2 ± 25.5 ng μL −1 ).…”

Section: Discussionmentioning

confidence: 46%

“…Pearson correlation coefficient and probability level are provided in Table . White culture description according to Adamcikova et al . (b) Growth rate measured as colony orthogonal diameters after 7 days incubation.…”

Section: Resultsmentioning

confidence: 99%

“…It has previously been reported that mycovirus infection (hypovirulence) in C. parasitica is associated with loss of colony pigmentation and wileyonlinelibrary.com/journal/ps (1) Switzerland (15) Bosnia (11) Slovenia (1) reduced sporulation. [34][35][36][37] However, it was notable in our study that the virus-infected UK isolates did not have a white colony phenotype and slower growth on initial screening, although low levels of CHV-1 could be detected (original nanograms per microlitre detections of 9.9 for L-6 and 9.2 for Db-1), but only became apparent on re-isolation from inoculated host material when the virus concentration was also found to have increased (respective concentrations of 31.5 ± 5.7 and 30.2 ± 25.5 ng μL −1 ). However, not all the phenotype changes could be correlated with virus concentration.…”

Section: Discussionmentioning

confidence: 99%

“…In Table 1. White culture description according to Adamcikova et al 34 Table 1. wileyonlinelibrary.com/journal/ps wileyonlinelibrary.com/journal/ps the ML analyses, the best fitted substitution model was K80+G with a gamma shape parameter of 2.687, whereas in MP analyses the number of parsimony informative characters was 264, the tree length 748, consistency index 0.639, retention index 0.617, and homoplasy index 0.361. BI analyses presented a burn-in value of 500 under the same substitution model than ML analyses.…”

Section: Haplotypesmentioning

confidence: 99%

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Hypovirulent effect of theCryphonectria hypovirus 1in British isolates ofCryphonectria parasitica

Romon-Ochoa

Gorton

Lewis

et al. 2019

Pest Management Science

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Background Chestnut blight, caused by Cryphonectria parasitica, is controlled in many European countries by the naturally occurring mycovirus Cryphonectria hypovirus 1 (CHV‐1). During surveys of recently identified chestnut blight outbreak in England, CHV‐1 was detected in several individuals of the pathogen isolated from affected trees. We investigated two of these CHV‐1‐infected isolates (L‐6 and Db‐1) as potential biocontrol agents for deployment in the UK comparing their virulence against virus‐free (M1275) and hypovirulent (M784) European isolates by inoculating sweet chestnut seedlings. Results Both the European CHV‐1 M784 hypovirulent isolate and UK L‐6 isolate formed significantly smaller lesions in sweet chestnut seedling bark than the other three isolates (Db‐1, and virulent isolates FTC121 and M1275). The highest virus concentration was detected in isolate M784, followed by L‐6, with the lowest concentration in isolate Db‐1. White colony colouration indicative of hypovirulence was common in colonies re‐isolated from smaller lesions, and the same isolates also tended to be slower growing in culture, have a higher virus concentration, and caused less epicormic growth and fewer stromata to be present in plants. L‐6 and Db‐1 virus sequences, respectively, matched the virus haplotype E‐5 detected previously in Switzerland and a mutation of the same subtype I haplotype. Conclusion Isolate L‐6 could potentially act as biocontrol for chestnut blight outbreaks in the UK but further laboratory and field experiments are needed. © 2019 Crown copyright. Pest Management Science © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 46%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Haplotypesmentioning

confidence: 99%

See 2 more Smart Citations

Hypovirulent effect of theCryphonectria hypovirus 1in British isolates ofCryphonectria parasitica

Romon-Ochoa

Gorton

Lewis

et al. 2019

Pest Management Science

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“…In most European countries the chestnut blight epidemic is restricted by hypovirus which infects C. parasitica and reduces its virulence. In Slovakia, chestnut blight remains a serious problem since natural hypovirulence of this disease by hypovirulent strains of C. parasitica has had little effect so far (Juhásová et al 2005;Adamčíková et al 2019). Similarly, selection of Castanea hybrids resistant to chestnut blight (Bolvanský et al 2014;Pažitný et al 2018) has not been.…”

Section: Laboratory Assaymentioning

confidence: 99%

Journal of Plant Protection Research

Adamčíková¹,

Jánošíková²,

Pažitný³

2020

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In the current study the antifungal activity of inorganic reagents was tested against Cryphonectria parasitica in vitro in a mycelial growth inhibition test. Three reagents, each consisting of chloride silver (AgCl) in combination with (1) aluminum oxide − Al 2 O 3 , (2) zinc oxide − ZnO, and (3) Al 2 O 3 and titanium dioxide -TiO 2 , were tested. Significant differences of the tested reagents on the growth of C. parasitica were recorded. The study demonstrated that silver in mixture with ZnO had an antifungal effect and significantly reduced the mycelial growth of C. parasitica in vitro. The mixture of AgCl with the other two combinations of inorganic metal oxides had no inhibition effect on the growth of the pathogen. It was confirmed that ZnO (applied in a single compound test) is responsible for inhibition of C. parasitica mycelium growth. A preliminary in planta assay was performed but statistically significant differences were not recorded in the average increment of canker length.

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Non-native tree species growth characteristics inventoried three decades after planting in the Danube Lowland

Longauer,

Belko,

Pástor

et al. 2023

Central European Forestry Journal

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This paper presents non-native (NN) coniferous and deciduous tree species growth and vitality assessment on experimental research plots established in 1980, 1982 and 1985 in the Danube Lowland over an extended time period. For NN coniferous tree species, Douglas fir, grand fir and eastern white pine reached significantly higher values of diameter at breast height (dbh) and height (H) than Austrian pine. For NN deciduous tree species markedly higher values of H and dbh for black walnut compared to sweet chestnut and red oak were recorded. Damage observed in larger extent on examined NN tree species stands included illegal removal of top of stem for grand fir and presence of necrosis on trunks of sweet chestnut. Comparison of NN coniferous and deciduous tree species growth with native reference tree species showed that NN Douglas fir, grand fir, eastern white pine, red oak and black walnut were capable to achieve similar or even higher values of assessed parameters than native reference tree species. The results suggest, that cultivation of NN coniferous, including Douglas fir, eastern white pine, as well as NN deciduous tree species, including red oak and black walnut in Danube Lowland could contribute to diversification and stabilisation of wood production potential of local forest formations.

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Hypovirulence in chestnut blight fungus,Cryphonectria parasitica, in Slovakia

Cited by 3 publications

References 43 publications

Hypovirulent effect of theCryphonectria hypovirus 1in British isolates ofCryphonectria parasitica

Hypovirulent effect of theCryphonectria hypovirus 1in British isolates ofCryphonectria parasitica

Journal of Plant Protection Research

Non-native tree species growth characteristics inventoried three decades after planting in the Danube Lowland

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