Hypoxia exerts oxidative stress and changes in expression of antioxidant enzyme genes in gills of <i>Mytilus galloprovincialis</i> (Lamarck, 1819)

Andreyeva, A. Yu.; Gostyukhina, O. L.; Кладченко, Е. С.; Afonnikov, D. A.; Рассказов, Д. А.; Лантушенко, А. О.; Водясова, Е. А.

doi:10.1080/17451000.2021.1967992

Cited by 15 publications

(6 citation statements)

References 69 publications

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“…In other bivalve species, hypoxia has been shown to affect amino acid and fatty acid catabolism in the mussel Mytilus edulis, while reoxygenation has resulted in increased mitochondrial respiration but a decrease in the production of reactive oxygen species (ROS), indicating the ability of this mussel species to cope with reoxygenation stress [50]. In the mussel M. galloprovincialis, hypoxia can decrease the activity of antioxidant enzymes but increase the upregulation of antioxidant enzyme genes, returning both (enzymes and genes) to basal levels during reoxygenation, suggesting tolerance of this mussel to hypoxia and reoxygenation stress [58,59]. The clam Scapharca inaequivalvis has also shown increased levels of enzymatic antioxidants during reoxygenation following anoxic conditions [60].…”

Section: Discussionmentioning

confidence: 99%

Oxidative Damage and Antioxidants as Markers for the Selection of Emersion Hardening Treatments in GreenshellTM Mussel Juveniles (Perna canaliculus)

Delorme,

Burritt,

Zamora

et al. 2024

Antioxidants

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Transport out of the water is one of the most challenging events for juvenile Perna canaliculus and can be a highly inefficient process, with many juveniles subsequently being lost following extended periods of emersion. Hardening techniques offer a possible method for reducing transport-related stress. In this study, different hardening treatments (short, long and intermittent sub-lethal emersion) were used to prepare ~1.2 mm P.canaliculus for transport (20 h) and subsequent reoxygenation stress during re-immersion (i.e., recovery). The oxidative stress responses, resettlement behaviour, respiration rates and survival of the mussels after transport and during recovery were all assessed. Short emersion (1 h) as a hardening treatment prior to transport did not cause major stress to the mussels, which maintained respiration at control levels, showed significantly stimulated antioxidant defences during recovery, showed greater resettlement behaviour and remained viable after 24 h of recovery. In comparison, the long and intermittent emersion treatments negatively impacted oxidative stress responses and affected the viability of the mussels after 24 h of recovery. This study showed that exposing juvenile P.canaliculus to a mild stress prior to transport may stimulate protective mechanisms, therefore eliciting a hardening response, but care must be taken to avoid overstressing the mussels. Improving the management of stress during the transport of juvenile mussels may be key to minimising mussel losses and increasing harvest production, and biomarkers associated with oxidative stress/antioxidant metabolism could be valuable tools to ensure emersion hardening does not overstress the mussels and reduce survival.

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Section: Discussionmentioning

confidence: 99%

Oxidative Damage and Antioxidants as Markers for the Selection of Emersion Hardening Treatments in GreenshellTM Mussel Juveniles (Perna canaliculus)

Delorme,

Burritt,

Zamora

et al. 2024

Antioxidants

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“…Hypoxia activates various molecular pathways in bivalve mollusks as an adaptive mechanism to restore oxygen homeostasis [ 100 ]. In recent decades, transcriptomic responses to hypoxia have been studied in several marine bivalve species [ 82 , 101 , 102 , 103 ]. In this study, a transcriptomic reaction was observed in the gills, adductor muscle, and digestive gland in response to hypoxic stress, indicating the importance of these tissues in regulating hypoxia in the Chilean mussel.…”

Section: Discussionmentioning

confidence: 99%

“…In recent decades, hypoxia has caused massive mortality and bivalve mollusks’ stranding along Chile’s central southern coast [ 22 , 70 , 71 , 72 ]. Therefore, understanding the tolerance mechanisms of bivalve mollusks to hypoxia is currently of utmost importance in contributing to the sustainability of this industry [ 3 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 ]. Thus, the effects of hypoxia on the physiological energetics, intermediary metabolites, cell survival, and inflammatory responses of the genus Mytilus suggest that hypoxia significantly affects the adaptation mechanisms of M. chilensis [ 3 , 29 , 74 ].…”

Section: Introductionmentioning

confidence: 99%

Hypoxia in the Blue Mussel Mytilus chilensis Induces a Transcriptome Shift Associated with Endoplasmic Reticulum Stress, Metabolism, and Immune Response

Montúfar-Romero,

Valenzuela-Muñoz,

Valenzuela-Miranda

et al. 2024

Genes

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The increase in hypoxia events, a result of climate change in coastal and fjord ecosystems, impacts the health and survival of mussels. These organisms deploy physiological and molecular responses as an adaptive mechanism to maintain cellular homeostasis under environmental stress. However, the specific effects of hypoxia on mussels of socioeconomic interest, such as Mytilus chilensis, are unknown. Using RNA-seq, we investigated the transcriptomic profiles of the gills, digestive gland, and adductor muscle of M. chilensis under hypoxia (10 days at 2 mg L−1) and reoxygenation (10 days at 6 mg L−1). There were 15,056 differentially expressed transcripts identified in gills, 11,864 in the digestive gland, and 9862 in the adductor muscle. The response varied among tissues, showing chromosomal changes in Chr1, Chr9, and Chr10 during hypoxia. Hypoxia regulated signaling genes in the Toll-like, mTOR, citrate cycle, and apoptosis pathways in gills, indicating metabolic and immunological alterations. These changes suggest that hypoxia induced a metabolic shift in mussels, reducing reliance on aerobic respiration and increasing reliance on anaerobic metabolism. Furthermore, hypoxia appeared to suppress the immune response, potentially increasing disease susceptibility, with negative implications for the mussel culture industry and natural bed populations. This study provides pivotal insights into metabolic and immunological adaptations to hypoxia in M. chilensis, offering candidate genes for adaptive traits.

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“…In recent years, with the development of the oyster industry summer mortality has been one of the most severe questions in farming practice. Previous studies indicated that the two major reasons for bivalve mass mortality were temperature increasing and dissolved oxygen decreasing (Parthasarathy et al, 1992;Joos et al, 2003;Soon and Zheng, 2019;Zhao et al, 2019;He et al, 2021), and hypoxia is considered to be one of the key factors affecting the survival of ocean organisms (Gu et al, 2019;Andreyeva et al, 2021). Benthic intertidal communities have developed appropriate survival mechanisms due to daily exposure into the air during low tide (Larade and Storey, 2002;Zhao et al, 2020), but over-farming, sediment covering, or algae blooming (Wu, 2002) could also cause water hypoxia.…”

Section: Introductionmentioning

confidence: 99%

“…Benthic intertidal communities have developed appropriate survival mechanisms due to daily exposure into the air during low tide (Larade and Storey, 2002;Zhao et al, 2020), but over-farming, sediment covering, or algae blooming (Wu, 2002) could also cause water hypoxia. Recent studies showed that the fluctuations of dissolved oxygen frequently influence physiological, metabolic, and cellular processes in organisms, and in this situation, oxidative stress becomes one of the reasons that cause cellular damage (Nogueira et al, 2017;Andreyeva et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Crassostrea sikamea (♀) × Crassostrea gigas (♂) Hybrids Under Hypoxia in Occluded Water

Zhang

Fan

et al. 2022

Front. Mar. Sci.

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Hypoxia is considered to be one of the key factors affecting the survival of ocean organisms, it is necessary to parse the molecular processes involved in response to hypoxia. As a potential breeding species, the hybrid of Crassostrea sikamea (♀) × Crassostrea gigas (♂) shows valuable heterosis in survival and growth traits. Thus, RNA de novo was deployed in this study to analyze the molecular processes in the hybrids under hypoxia stress. The hybrids were cultured in occluded water, then the dissolved oxygen was gradually consumed by oysters, and the gill tissue of hybrids was sampled at the very beginning and the lowest respiration point in the experiment. In the current study, 901 significant differentially expressed genes (DEGs) were identified under hypoxia compared to normoxia, among which 432 DEGs were downregulated, and the other 469 DEGs were upregulated. A total of 27 GO terms were significantly enriched, such as an integral component of membrane, extracellular region, immune response, tumor necrosis factor receptor binding, and neurotransmitter: sodium symporter activity. Besides, 19 KEGG pathways were significantly enriched, such as apoptosis, Th1 and Th2 cell differentiation, complement, and coagulation cascades, antigen processing and presentation, notch signaling pathway, and cytokine–cytokine receptor interaction. The current results showed that the TRAIL genes were downregulated, but the HSP70 and LIGHT genes were upregulated, which indicated the inhibition of Apoptosis, and the activity of innate immunity in oysters under hypoxia. This study provides preliminary insight into the molecular response to hypoxia in the gill of hybrids.

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Hypoxia exerts oxidative stress and changes in expression of antioxidant enzyme genes in gills of Mytilus galloprovincialis (Lamarck, 1819)

Cited by 15 publications

References 69 publications

Oxidative Damage and Antioxidants as Markers for the Selection of Emersion Hardening Treatments in GreenshellTM Mussel Juveniles (Perna canaliculus)

Oxidative Damage and Antioxidants as Markers for the Selection of Emersion Hardening Treatments in GreenshellTM Mussel Juveniles (Perna canaliculus)

Hypoxia in the Blue Mussel Mytilus chilensis Induces a Transcriptome Shift Associated with Endoplasmic Reticulum Stress, Metabolism, and Immune Response

Transcriptome Analysis of Crassostrea sikamea (♀) × Crassostrea gigas (♂) Hybrids Under Hypoxia in Occluded Water

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