Hypoxia increases vegf secretion in multiple sources of mesenchymal stem cell

Lagonda, Christine Ayu; Tjahjadi, F.B.; Fauza, Dilafitria; Kusnadi, Yuyus

doi:10.1016/j.jcyt.2018.02.114

Cited by 5 publications

(2 citation statements)

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“…We then demonstrated that VEGF levels in the hypoxia conditioning media of human UC-MSC were significantly increased compared to normoxic cells, suggesting hypoxia may stimulate the expression of VEGF within UC-MSC. Our data further support previous studies showing that hypoxia not only affects the VEGF expression in hUC-derived MSC [ 20 ] but also in various sources of MSC including adipose and bone marrow [ 21 , 22 ]. Vascular endothelial growth factor (VEGF) is a potent angiogenic factor that promotes neovascularization by stimulating vasculogenesis, angiogenesis and vascular permeability.…”

Section: Discussionsupporting

confidence: 92%

Hipoxia modulates the secretion of growth factors in of human umbilical cord-derived mesenchymal stem cells

Arfianti,

Ulfah,

Hutabarat

et al. 2023

BioMedicine

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Background Mesenchymal stem cell (MSC) has great potential as therapies due its ability to regenerate tissue damage and promote tissue homeostasis. Preconditioning of MSC in low oxygen concentration has been shown to affect the therapeutic potential of these cells. This study aimed to compare the characteristic and secretion of trophic factors of MSCs cultured under hypoxia and normoxia. Methods MSCs were isolated from Wharton’s jelly of human umbilical cord (UC) tissue by explant method and characterized by flow cytometry. Following 24 h of CoCl 2 -induced hypoxic culture, the viability and metabolic activity of MSC were analyzed by trypan blue exclusion test and methyl thiazolyl tetrazolium (MTT) assay, respectively. The secretion of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) was assessed in conditioned medium using enzyme-linked immunosorbent assay (ELISA) method. Results Flow cytometry analysis showed >99% of the population of MSCs cells were positive for CD73 and CD90 and > 62% were positive for CD105. While the cell viability of MSC was not affected by hypoxic cultured condition, the metabolic activity rate of these cells was decreased under hypoxic conditioning. In line with reduced metabolic activity, hypoxic human UC-derived MSC produced less HGF than normoxic counterpart. Compared to normoxic MSC, hypoxic preconditioned MSC secreted higher level of VEGF in the conditioned medium (p < 0.05). Conclusions Hypoxia decreased the metabolic activity of MSCs associated with the modulation of HGF and VEGF secretions. It is suggested that hypoxia may also affect the therapeutic capacity of MSC cells.

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Section: Discussionsupporting

confidence: 92%

Hipoxia modulates the secretion of growth factors in of human umbilical cord-derived mesenchymal stem cells

Arfianti,

Ulfah,

Hutabarat

et al. 2023

BioMedicine

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“…This observation was similar to MSCs from the bone marrow 34 . Lagonda et al (2018) also showed that at a 3% oxygen level, MSCs from umbilical cord tissue, bone marrow, and adipose tissue significantly increased VEGF synthesis and secretion compared to normoxia 35 . The mechanism under this observation is related to the activation of HIF-1 alpha caused by hypoxia 36 .…”

Section: Discussionmentioning

confidence: 99%

Conditioned media from human adipose-derived stem cell culture in some stressed culture conditions differ angiogenic potential

Nguyen

et al. 2021

Biomed. Res. Ther.

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Introduction: The potency of mesenchymal stem cells (MSCs) related to their biological effects includes immune modulation and angiogenesis. Recently, some stress conditions were applied to enhance the angiogenic potential of MSCs. This study aimed to assess the effects of conditioned media (CM) collected from adipose-derived stem cells’ (ADSCs’) culture under various stress conditions on angiogenesis in vitro. Methods: ADSCs were isolated and expanded according to a published protocol. These cells were treated with some stress conditions including hypoxia, starvation, a combination of hypoxia and starvation, and TNF-alpha treatment. CM from these cultures were collected and used for further experiments. The angiogenic potential of CM was evaluated through stimulation of HUVECs to form vessels in vitro. ELISA was used to measure the VEGF concentrations in CM. Results: CM-derived various stress ADSC cultures differently affected angiogenesis of HUVECs. The supernatant from a hypoxic culture of ADSCs contained the highest concentration of VEGF and was higher than normoxic culture. However, in others, VEGF concentrations in CM significantly reduced compared to control. CM from TNF-alpha treatment failed to support the formation of blood vessels from HUVECs, while other conditions could support blood vessel formation in vitro. TNF-alpha dually affected both ADSCs and HUVECs. Furthermore, TNF-alpha could stimulate or suppress the VEGF production in dose-response in ADSCs and cause apoptosis in HUVECs at high concentrations. Conclusion: CM from the hypoxic culture of ADSCs contained a high concentration of VEGF, supporting angiogenesis of HUVECs well. This is a simple technique that can be used in translational applications. However, the use of TNF-alpha yielded dual effects on ADSCs and HUVECs. Although the VEGF production was enhanced at a low dose of TNF-alpha, they could induce apoptosis in endothelial cells to cause the failure of angiogenesis.

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The VEGF‐Mediated Cytoprotective Ability of MIF‐Licensed Mesenchymal Stromal Cells in House Dust Mite‐Induced Epithelial Damage

Dunbar,

Hawthorne,

Tunstead

et al. 2024

Eur J Immunol

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Enhancing mesenchymal stromal cell (MSC) therapeutic efficacy through licensing with proinflammatory cytokines is now well established. We have previously shown that macrophage migration inhibitory factor (MIF)‐licensed MSCs exerted significantly enhanced therapeutic efficacy in reducing inflammation in house dust mite (HDM)‐driven allergic asthma. Soluble mediators released into the MSC secretome boast cytoprotective properties equal to those associated with the cell itself. In asthma, epithelial barrier damage caused by the inhalation of allergens like HDM drives goblet cell hyperplasia. Vascular endothelial growth factor (VEGF) plays a pivotal role in the repair and maintenance of airway epithelial integrity. Human bone marrow‐derived MSCs expressed the MIF receptors CD74, CXCR2, and CXCR4. Endogenous MIF from high MIF expressing CATT7 bone marrow‐derived macrophages increased MSC production of VEGF through the MIF CXCR4 chemokine receptor, where preincubation with CXCR4 inhibitor mitigated this effect. CATT7‐MIF licensed MSC conditioned media containing increased levels of VEGF significantly enhanced bronchial epithelial wound healing via migration and proliferation in vitro. Blocking VEGFR2 or the use of mitomycin C abrogated this effect. Furthermore, CATT7‐MIF MSC CM significantly decreased goblet cell hyperplasia after the HDM challenge in vivo. This was confirmed to be VEGF‐dependent, as the use of anti‐human VEGF neutralising antibody abrogated this effect. Overall, this study highlights that MIF‐licenced MSCs show enhanced production of VEGF, which has the capacity to repair the lung epithelium.

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Hypoxia increases vegf secretion in multiple sources of mesenchymal stem cell

Cited by 5 publications

References 0 publications

Hipoxia modulates the secretion of growth factors in of human umbilical cord-derived mesenchymal stem cells

Hipoxia modulates the secretion of growth factors in of human umbilical cord-derived mesenchymal stem cells

Conditioned media from human adipose-derived stem cell culture in some stressed culture conditions differ angiogenic potential

The VEGF‐Mediated Cytoprotective Ability of MIF‐Licensed Mesenchymal Stromal Cells in House Dust Mite‐Induced Epithelial Damage

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