Hypoxia induced altered expression of heat shock protein genes (Hsc71, Hsp90α and Hsp10) in Indian Catfish, Clarias batrachus (Linnaeus, 1758) under oxidative stress

Abstract

Heat shock proteins (Hsps) are typically associated with stress response and tolerance. The Indian catfish, Clarias batrachus, is a freshwater air-breathing hypoxia tolerant teleost and is potentially important catfish species for aquaculture and for its economic value as food. The present study aimed at determining the transcriptional response of three Hsps, CbHsc71, CbHsp90α and CbHsp10 in hypoxia tolerant Indian catfish, C. batrachus, under experimental and natural hypoxia. The expression profile of above three genes were studied under different periods of hypoxia, through qRT-PCR. Primers were designed from ESTs obtained through SSH libraries constructed from hypoxia treated fishes. The Hsp10 ESTs and deduced protein was in silico characterized for its ORF and for its physical and chemical properties, respectively, using GeneScan, blastp, scanprosite, superfamily and other softwares. A phylogenetic tree was constructed based on deduced amino acid sequences of Hsc71, Hsp90α, Hsp90β of Homo sapiens and other fishes along with CbHsp10 protein in MEGA4. The deduced protein sequences of CbHsp10 was found to have characteristic Hsp10 family signatures, and it is proposed for inclusion of methionine in the consensus sequences of Hsp10 family signature, after the "proline" residue. At transcription level, these genes were found to be differentially regulated under hypoxia stress, in different tissues of C. batrachus. The CbHsc71 and CbHsp90α were up-regulated after short and long-term hypoxia, whereas CbHsp10 was significantly down-regulated after short-term hypoxia. The differential expression of these Hsps may play a role in protection and survival under hypoxia induced oxidative stress in C. batrachus.

Deep Transcriptomic Analysis of Black Rockfish (Sebastes schlegelii) Provides New Insights on Responses to Acute Temperature Stress

“…It is interesting that the heat shock cognate 70–2 ( hsc70 ) was enriched in 5 KEGG pathways (corrected p-value < 0.05): influenza a (ko05164); legionellosis (ko05134); estrogen signaling pathway (ko04915); measles (ko05162); antigen processing and presentation (ko04612), indicate that hsc70 plays a comprehensive role in the acute thermal stress response, a result that has been shown in different species 4 , 37 , 38 . Similar to the present results, other studies have reported that hsc71 is up-regulated after hypoxia stress 39 . Interleukin 1 beta (IL-1β) is an evolutionarily conserved molecule originally identified in the immune system, and it plays a critical role in the activation of immune cells 40 .…”

Proteomic profiling of ascidians as a tool for biomonitoring marine environments

“…mytiligera from its substrate may also account for a different pattern of protein expression. The list of significant proteins nonetheless includes potential biomarkers for various stressors such as actin [44], 14-3-3 proteins [37], and Hsc71 [45].…”

The cellular stress response of the scleractinian coral Goniopora columna during the progression of the black band disease

“…On the contrary, during the night, biogeochemical conditions at the base of the mat are typically hypoxic or anoxic with high levels of sulfide and low pH (reviewed in Sato et al 2016). It is well known that usually both hyperoxia and hypoxia, as well as the exposition to sulfide, may lead to the upregulation of the antioxidant systems HO-1 and MnSOD as well as that of Hsps (Lee et al 1997;Yamashita et al 1997;Carraway et al 2000;Garrido et al 2001;Richier et al 2003;Calvert et al 2009;Mohindra et al 2015). Moreover, sulfide is toxic to most eukaryotes, inhibiting electron transport in aerobic respiration, leading to apoptosis and tissue necrosis, and increasing the cellular oxidative stress (Vismann 1991).…”