Hypoxia-induced Synthesis of Hemoglobin in the Crustacean Daphnia magna Is Hypoxia-inducible Factor-dependent

Gorr, Thomas A.; Cahn, Joshua D.; Yamagata, Hideo; Bunn, H. Franklin

doi:10.1074/jbc.m403981200

Cited by 110 publications

(98 citation statements)

References 64 publications

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“…A. Michelson and N. Perrimon. The cells were cultured as described in the accompanying article (87). SL2 cells were grown at 22°C in air either as monolayer or suspension cultures.…”

Section: Methodsmentioning

confidence: 99%

“…those capable of oxygen-dependent binding of HIF proteins in vitro and transactivating reporter genes in vivo, can be summarized in the following consensus sequence: 5Ј-B(A/G)CGT-GVBBB-3Ј (where B is all bases except A and V is all bases except T) (40). The central 4-base core (underlined) is critical for the binding of any HIF complex, be it mammalian, insect, or crustacean (see accompanying article (87)). …”

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confidence: 99%

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Regulation of Drosophila Hypoxia-inducible Factor (HIF) Activity in SL2 Cells

Gorr

Tomita

Wappner

et al. 2004

Journal of Biological Chemistry

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Although hypoxia-inducible factor-␣ (HIF␣) subunitspecific hydroxylation and proteolytic breakdown explain the binary switch between the presence (hypoxia) and absence (normoxia) of HIFs, little is known of the mechanisms that fine-tune HIF activity under constant, rather than changing, oxygen tensions. Here, we report that the Drosophila HIF␣ homolog, the basic helix-loophelix/PAS protein Sima (Similar), in hypoxic cultures of SL2 cells is expressed in full-length (fl) and splice variant (sv) isoforms. The following evidence supports the role of flSima as functional HIF␣ and the role of SL2 HIF as a transcriptional activator or suppressor. The pO 2 dependence of Sima abundance matched that of HIF activity. HIF-dependent changes in candidate target gene expression were detected through variously effective stimuli: hypoxia (strong) > iron chelation, e.g. desferrioxamine (moderate) > > transition metals, e.g. cobalt Ӎ normoxia (ineffective). Sima overexpression augmented hypoxic induction or suppression of different targets. In addition to the full-length exon 1-12 transcript yielding the 1510-amino acid HIF␣ homolog, the sima gene also expressed, specifically under hypoxia, an exon 1-7/12 splice variant, which translated into a 426-amino acid Sima truncation termed svSima. svSima contains basic helix-loop-helix and PAS sequences identical to those of flSima, but, because of deletion of exons 8 -11, lacks the oxygen-dependent degradation domain and nuclear localization signals. Overexpressed svSima failed to transactivate reporter genes. However, it attenuated HIF (Sima⅐Tango)-stimulated reporter expression in a dose-dependent manner. Thus, svSima has the potential to regulate Drosophila HIF function under steady and hypoxic pO 2 by creating a cytosolic sink for the Sima partner protein Tango.

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“…A. Michelson and N. Perrimon. The cells were cultured as described in the accompanying article (87). SL2 cells were grown at 22°C in air either as monolayer or suspension cultures.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Regulation of Drosophila Hypoxia-inducible Factor (HIF) Activity in SL2 Cells

Gorr

Tomita

Wappner

et al. 2004

Journal of Biological Chemistry

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“…These results, plus the identification of putative binding sites for the mammalian hypoxia-inducible factor 1 (HIF-1; Kimura et al, 1999), led to a detailed investigation of Hb gene promoters. Induction of Hb gene expression during hypoxia is dependent on the binding of HIF to particular hypoxia response elements (HRE) in promoter regions of the four known D. magna Hb genes (Gorr et al, 2004). Three HREs upstream of the globin-2 gene were found using heterologous transfection of HIF-expressing human and D. melanogaster cells, showing specific binding of HIF complexes at two sites in vitro and binding of an unknown constitutive transcription factor at the third site (Gorr et al, 2004).…”

Section: Genomics Of Environmental Stress Responsesmentioning

confidence: 99%

“…Induction of Hb gene expression during hypoxia is dependent on the binding of HIF to particular hypoxia response elements (HRE) in promoter regions of the four known D. magna Hb genes (Gorr et al, 2004). Three HREs upstream of the globin-2 gene were found using heterologous transfection of HIF-expressing human and D. melanogaster cells, showing specific binding of HIF complexes at two sites in vitro and binding of an unknown constitutive transcription factor at the third site (Gorr et al, 2004). A recent report (Gorr et al, 2006) has identified a putative juvenoid response element (JRE) in the promoter of hb2 in D. magna that binds an activated factor in response to JHA.…”

Section: Genomics Of Environmental Stress Responsesmentioning

confidence: 99%

Ecological genomics in Daphnia: stress responses and environmental sex determination

2007

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Ecological genomics is the study of adaptation of natural populations to their environment, and therefore seeks to link organism and population level processes through an understanding of genome organization and function. The planktonic microcrustacean Daphnia, which has long been an important system for ecology, is now being used as a genomic model as well. Here we review recent progress in selected areas of Daphnia genomics research. Production of parthenogenetic male offspring occurs through environmental cues, which clearly involves endocrine regulation and has also been studied as a toxicological response to juvenoid hormone analog insecticides. Recent progress has uncovered a putative juvenoid cis-response element, which together with microarray analysis will stimulate further research into nuclear hormone receptors and their associated transcriptional regulatory networks. Ecotoxicological studies indicate that mRNA profiling is a sensitive and specific research tool with promising applications in environmental monitoring and for uncovering conserved cellular processes. Rapid progress is expected to continue in these and other areas, as genomic tools for Daphnia become widely available to investigators.

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“…3 Hypoxic regulation of Hb synthesis has been observed for certain Hb chains in several species in vitro and in vivo and in some cases may involve the hypoxia-inducible factor signaling pathway. 7,8 Neuroglobin and cytoglobin, two more recently described globin family members, are also hypoxically induced and confer cytoprotection during ischemia. 9,10 Whether hypoxia modulates the biologic function of mesangial Hb remains to be investigated.…”

mentioning

confidence: 99%

Hemoglobin in the Kidney

Haase

2008

Journal of the American Society of Nephrology

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Hypoxia-induced Synthesis of Hemoglobin in the Crustacean Daphnia magna Is Hypoxia-inducible Factor-dependent

Cited by 110 publications

References 64 publications

Regulation of Drosophila Hypoxia-inducible Factor (HIF) Activity in SL2 Cells

Regulation of Drosophila Hypoxia-inducible Factor (HIF) Activity in SL2 Cells

Ecological genomics in Daphnia: stress responses and environmental sex determination

Hemoglobin in the Kidney

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