Hypoxia-inducible factor-1α in Antarctic notothenioids contains a polyglutamine and glutamic acid insert that varies in length with phylogeny

Rix, Anna S.; Grove, Theresa J.; O’Brien, Kristin M.

doi:10.1007/s00300-017-2164-6

Cited by 6 publications

(2 citation statements)

References 49 publications

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“…This highly tuned system, discovered in mammals, plays a notable role in the hypoxia response of fish. Analysis of icefish mRNA revealed that the functional domains of Hif-1-alpha are highly conserved, indicating continued selective pressure exerted by hypoxia (Rix et al, 2017). These and other hypoxia response gene relationships have been characterized in detail in the hypoxic fish database, HRGFish, which provides a variety of prediction tools and validation methods to further evaluate the conservation of hypoxia genes in diverse fish species (Rashid et al, 2017).…”

Section: Fish Cell Culturementioning

confidence: 99%

Cell-Based Fish: A Novel Approach to Seafood Production and an Opportunity for Cellular Agriculture

Rubio

Datar

Stachura

et al. 2019

Front. Sustain. Food Syst.

100

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Cellular agriculture is defined as the production of agricultural products from cell cultures rather than from whole plants or animals. With growing interest in cellular agriculture as a means to address public health, environmental, and animal welfare challenges of animal agriculture, the concept of producing seafood from fish cell-and tissue-cultures is emerging as an approach to address similar challenges with industrial aquaculture systems and marine capture. Cell-based seafood-as opposed to animal-based seafood-can combine developments in biomedical engineering with modern aquaculture techniques. Biomedical engineering developments such as closed-system bioreactor production of land animal cells create a basis for the large scale production of marine animal cells. Aquaculture techniques such as genetic modification and closed system aquaculture have achieved significant gains in production that can pave the way for innovations in cell-based seafood production. Here, we present the current state of innovation relevant to the development of cell-based seafood across multiple species, as well as specific opportunities and challenges that exist for advancing this science. The authors find that the physiological properties of fish celland tissue-culture may be uniquely suited to cultivation in vitro. These physiological properties, including tolerance to hypoxia, high buffering capacity, and low-temperature growth conditions, make marine cell culture an attractive opportunity for scaled production of cell-based seafood; perhaps even more so than mammalian and avian cell cultures for cell-based meats. This opportunity, coupled with the unique capabilities of crustacean tissue-friendly scaffolding such as chitosan, a common seafood waste product and mushroom derivative, presents promise for cell-based seafood production via bioreactor cultivation. To become fully realized, cell-based seafood research will require more understanding of fish muscle cell and tissue cultivation; more investigation into serum-free media formulations optimized for fish cell culture; and bioreactor designs tuned to the needs of fish cells for large scale production.

show abstract

Section: Fish Cell Culturementioning

confidence: 99%

Cell-Based Fish: A Novel Approach to Seafood Production and an Opportunity for Cellular Agriculture

Rubio

Datar

Stachura

et al. 2019

Front. Sustain. Food Syst.

100

View full text Add to dashboard Cite

show abstract

“…This highly tuned system, while discovered in mammals, plays a notable role in the hypoxia response of fish. Analysis of icefish mRNA revealed that the functional domains of Hif1-alpha are highly conserved indicating the continued selective pressure exerted by hypoxia (Rix, Grove, and O'Brien 2017). These and other hypoxia response gene relationships and predictions have been characterized in greater detail in the hypoxic fish database, HRGFish, available online, which provides a variety of prediction tools and validation methods to further evaluate the conservation of hypoxia genes in diverse fish species (Rashid et al 2017) In the absence of research on the oxygen requirements of fish cell culture in bioreactor systems, the authors speculate that the multitude of genetic adaptations of fish to staggering levels of hypoxia suggest that fish tissues may be uniquely suited to the oxygen-limited environments found in tissue culture and bioreactor conditions.…”

Section: Of Fishmentioning

confidence: 99%

Cell-Based Fish: A Novel Approach to Seafood Production and an Opportunity for Cellular Agriculture

Rubio¹,

Datar²,

Stachura³

et al. 2019

Preprint

View full text Add to dashboard Cite

Cellular agriculture is defined as the production of agricultural products from cell cultures rather than from whole plants or animals. With growing interest in cellular agriculture as a means to address the public health, environmental, and animal welfare challenges of animal agriculture, the concept of producing seafood from fish cell- and tissue-cultures is emerging as a means to address similar challenges with industrial aquaculture systems and marine capture. Cell-based seafood - as opposed to animal-based seafood - can combine developments in biomedical engineering with modern aquaculture techniques. Biomedical engineering developments such as closed-system bioreactor production of land animal cells create a basis for large scale production of marine animal cells. Aquaculture techniques such as genetic modification and closed system aquaculture have achieved marked gains in production that can pave the way for innovations in cell-based seafood production. Here, we present the current state of innovation relevant to the development of cell-based seafood across multiple species as well as specific opportunities and challenges that exist for advancing this science. The authors find that the physiological properties of fish cell- and tissue- culture may be uniquely suited to cultivation in vitro. These physiological properties, including hypoxia tolerance, high buffering capacity, and low-temperature growth conditions, make marine cell culture an attractive opportunity for scale production of cell-based seafood; perhaps even more so than mammalian and avian cell cultures for cell-based meats. This, coupled with the unique capabilities of crustacean tissue-friendly scaffolding such as chitosan, a common seafood waste product and mushroom derivative, presents great promise for cell-based seafood production via bioreactor cultivation. To become fully realized, cell-based seafood research will require more understanding of fish muscle culture and cultivation; more investigation into serum-free media formulations optimized for fish cell culture; and bioreactor designs tuned to the needs of fish cells for large scale production.

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The hypoxia response pathway in the Antarctic fish Notothenia coriiceps is functional despite a poly Q/E insertion mutation in HIF-1α

O'Brien,

Rix,

Jasmin

et al. 2024

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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Hypoxia-inducible factor-1α in Antarctic notothenioids contains a polyglutamine and glutamic acid insert that varies in length with phylogeny

Cited by 6 publications

References 49 publications

Cell-Based Fish: A Novel Approach to Seafood Production and an Opportunity for Cellular Agriculture

Cell-Based Fish: A Novel Approach to Seafood Production and an Opportunity for Cellular Agriculture

Cell-Based Fish: A Novel Approach to Seafood Production and an Opportunity for Cellular Agriculture

The hypoxia response pathway in the Antarctic fish Notothenia coriiceps is functional despite a poly Q/E insertion mutation in HIF-1α

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