Hypoxia-inducible factor 3-alpha expression (HIF-3α) is associated with the stable chondrocyte phenotype and inhibits HIF-2α/ARNTL-mediated transactivation of matrix metalloproteinase-13

Markway, Brandon D.; Cho, H.; Anderson, Deirdre E.J.; Zilberman‐Rudenko, Jevgenia; Holden, Peter R.; McAlinden, Audrey; Otero, Miguel; Goldring, Mary B.; Johnstone, Brian

doi:10.1016/j.joca.2015.02.906

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Arntl, also known as Hif-1b, activates hypertrophic related genes like MMP13, COL10A1, and VEGFA when dimerized with Hif-2a; however, competitive binding by Hif-3a suppresses transcriptional activation. 57 Knockdown of BCAT1 expression reduced proliferation, cell migration, and invasion of human nasopharyngeal carcinoma cells. 58 CDK1 is involved in cell cycle regulation, specifically the G1 to S transition.…”

Section: Fig 7 Top 50mentioning

confidence: 99%

Transcriptomic Analysis of Human Mesenchymal Stem Cell Therapy in Incontinent Rat Injured Urethra

Sadeghi

Kenyon

Richardson

et al. 2020

Tissue Engineering Part A

View full text Add to dashboard Cite

Periurethral human mesenchymal stem cell (hMSC) injections are associated with functional improvement in animal models of postpartum stress urinary incontinence (SUI). However, limited data exist on the role of hMSCs in modulating gene expression in tissue repair after urethral injury. To this end, we quantified temporal gene expression modulation in hMSCs, and in injured rat urethral tissue, using RNA-seq in an animal model of SUI, over a 3-day period following urethral injury, and local hMSC injection. We injected PKH fluorescent-labeled hMSC into the periurethral space of rats following a 4 h vaginal distention (VD) (three rats per time point). Control rats underwent VD injury only, and all animals were euthanized at 12, 24, 36, 72 h postinjury. Rat urethral and vaginal tissues were frozen and sectioned. Fluorescent labeled hMSCs were distinguished from adjacent, unlabeled rat urethral tissue. RNA was prepared from hMSCs and urethral tissue obtained by laser dissection of frozen tissue sections and sequenced on an Illumina HiSeq 2500. Differentially expressed genes (DEGs) over 72 h were evaluated using a two-group t-test (p < 0.05). Our transcriptional analyses identified candidate genes involved in tissue injury that were broadly sorted by injury and exposure to hMSC throughout the first 72 h of acute phase of injury. DEGs in treated urethra, compared with untreated urethra, were functionally associated with tissue repair, angiogenesis, neurogenesis, and oxidative stress suppression. DEGs included a variety of cytokines, extracellular matrix stabilization and regeneration genes, cytokine signaling modification, cell cycle regulation, muscle differentiation, and stabilization. Moreover, our results revealed DEG changes in hMSCs (PKH-labeled) harvested from injured urethra. The expressions are related to DNA damage repair, transcription activation, stem cell regulation, cell survival, apoptosis, self-renewal, cell proliferation, migration, and injury response.

show abstract

Section: Fig 7 Top 50mentioning

confidence: 99%