Hypoxia-mimicking scaffolds with controlled release of DMOG and PTHrP to promote cartilage regeneration via the HIF-1α/YAP signaling pathway

Chen, Li; Chen, Hong; Bao, Dingsu; Su, Xudong; Li, Wei; Hu, Ning; Huang, Wei; Xiang, Zhou

doi:10.1016/j.ijbiomac.2022.12.094

Cited by 7 publications

(15 citation statements)

References 54 publications

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“…The compound DMOG has been increasingly utilized in the field of tissue engineering, predominantly attributed to its capability to promote angiogenesis through the stabilization of Hypoxia-Inducible Factor-1 alpha (HIF-1α) [33,34] . Nonetheless, the current study elucidates the advantageous impact of DMOG on regenerative processes extending beyond angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

The dimethyloxalylglycine-functionalized nanofibers for in situ regeneration of infected developing dental roots

Woo,

Yoo,

Eun-Hye

et al. 2024

Preprint

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In situ regeneration in restorative dentistry focuses on repairing tissues directly at the injury site by using engineered biomaterials to guide endogenous cell activity. This approach aims to simplify treatment processes and improve outcomes for conditions like developing teeth with necrotic pulp infections. This study explores the potential of poly(ε-caprolactone) fibers (PCLF) functionalized with dimethyloxalylglycine (DMOG) for in situ regeneration in the context of dental root repair in immature teeth with necrotic pulp and apical periodontitis. In vivo application to a model in beagle dogs demonstrated the effectiveness of PCLF/DMOG in promoting root development, apical closure, and clearing infectious lesions, contrasting with calcium hydroxide paste, contemporary regenerative endodontic treatment controls that showed thin root growth and resilient persistent infection. Mechanistically, the sustained release of DMOG from PCLF/DMOG significantly enhanced the expression of hypoxia-inducible factor 1-alpha and upregulated genes associated with angiogenesis and neurogenesis, including vascular endothelial growth factor-A and nerve growth factor. The PCLF/DMOG upregulated antimicrobial peptides, facilitated efferocytic activities, and promoted macrophage polarization to the M2 phenotype. These findings highlight the potential of PCLF/DMOG scaffolds for dental root regeneration, offering a promising approach for treating immature teeth with necrotic pulp and apical periodontitis through in situ regeneration.

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Section: Discussionmentioning

confidence: 99%

The dimethyloxalylglycine-functionalized nanofibers for in situ regeneration of infected developing dental roots

Woo,

Yoo,

Eun-Hye

et al. 2024

Preprint

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“…Human-derived BMSCs were used in nine studies [31,55,60,65,[70][71][72][73][74], while rat-derived BMSCs were used in eight studies [32,43,44,47,54,58,64,77]. There is limited research on mouse-derived [63], pig-derived [75], and rabbit-derived [76] BMSCs. Apart from BMSCs, ADSCs have also been investigated for bone tissue regeneration.…”

Section: Isolation and Characterization Of Mscsmentioning

confidence: 99%

“…A total of 20 in vivo experimental studies meeting the inclusion criteria were reviewed [42][43][44][45]47,[52][53][54]58,59,61,62,66,68,[71][72][73][75][76][77]. Among these, 13 studies utilized rats as the animal model [42][43][44][45]54,59,61,62,66,68,71,72,77], 5 studies used mice [52,61,62,75,76], and two studies used rabbits [53,58]. The defect models varied across the studies, with nine studies inducing skull defects [42,44,45,59,61,62,66,71,72], 2 studies creating mandibular defect models…”

Section: In Vivo Studiesmentioning

confidence: 99%

Effect of Dimethyloxalylglycine on Stem Cells Osteogenic Differentiation and Bone Tissue Regeneration—A Systematic Review

Dong,

Fei,

Zhang

et al. 2024

IJMS

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Dimethyloxalylglycine (DMOG) has been found to stimulate osteogenesis and angiogenesis of stem cells, promoting neo-angiogenesis in bone tissue regeneration. In this review, we conducted a comprehensive search of the literature to investigate the effects of DMOG on osteogenesis and bone regeneration. We screened the studies based on specific inclusion criteria and extracted relevant information from both in vitro and in vivo experiments. The risk of bias in animal studies was evaluated using the SYRCLE tool. Out of the 174 studies retrieved, 34 studies met the inclusion criteria (34 studies were analyzed in vitro and 20 studies were analyzed in vivo). The findings of the included studies revealed that DMOG stimulated stem cells’ differentiation toward osteogenic, angiogenic, and chondrogenic lineages, leading to vascularized bone and cartilage regeneration. Addtionally, DMOG demonstrated therapeutic effects on bone loss caused by bone-related diseases. However, the culture environment in vitro is notably distinct from that in vivo, and the animal models used in vivo experiments differ significantly from humans. In summary, DMOG has the ability to enhance the osteogenic and angiogenic differentiation potential of stem cells, thereby improving bone regeneration in cases of bone defects. This highlights DMOG as a potential focus for research in the field of bone tissue regeneration engineering.

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“…To this end, we have proposed the utilization of microspheres to achieve sustained delivery of Bevacizumab and IGF-1. As a well-established system for sustained drug release, poly (lactic-co-glycolic acid) (PLGA) microspheres are capable of delivering the loaded drugs in a slow and continuous manner ( Morille et al, 2016 ; Su et al, 2021 ; Chen et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Injectable hydrogel loaded with bilayer microspheres to inhibit angiogenesis and promote cartilage regeneration for repairing growth plate injury

Qiang

Fan

Wang

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: The repair and regeneration of growth plate injuries using tissue engineering techniques remains a challenge due to large bone bridge formation and low chondrogenic efficiency.Methods: In this study, a bilayer drug-loaded microspheres was developed that contains the vascular endothelial growth factor (VEGF) inhibitor, Bevacizumab, on the outer layer and insulin-like growth factor-1 (IGF-1), a cartilage repair factor, on the inner layer. The microspheres were then combined with bone marrow mesenchymal stem cells (BMSCs) in the gelatin methacryloyl (GelMA) hydrogel to create a composite hydrogel with good injectability and biocompatibility.Results: The in vitro drug-release profile of bilayer microspheres showed a sequential release, with Bevacizumab released first followed by IGF-1. And this hydrogel simultaneously inhibited angiogenesis and promoted cartilage regeneration. Finally, in vivo studies indicated that the composite hydrogel reduced bone bridge formation and improved cartilage regeneration in the rabbit model of proximal tibial growth plate injury.Conclusion: This bilayer microsphere-based composite hydrogel with sequential controlled release of Bevacizumab and IGF-1 has promising potential for growth plate injury repair.

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Hypoxia-mimicking scaffolds with controlled release of DMOG and PTHrP to promote cartilage regeneration via the HIF-1α/YAP signaling pathway

Cited by 7 publications

References 54 publications

The dimethyloxalylglycine-functionalized nanofibers for in situ regeneration of infected developing dental roots

The dimethyloxalylglycine-functionalized nanofibers for in situ regeneration of infected developing dental roots

Effect of Dimethyloxalylglycine on Stem Cells Osteogenic Differentiation and Bone Tissue Regeneration—A Systematic Review

Injectable hydrogel loaded with bilayer microspheres to inhibit angiogenesis and promote cartilage regeneration for repairing growth plate injury

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