Hypoxia pretreatment improves the therapeutic potential of bone marrow mesenchymal stem cells in hindlimb ischemia via upregulation of NRG-1

Injectable hypoxia-preconditioned cartilage progenitor cells-laden GelMA microspheres system for enhanced osteoarthritis treatment

“…Thus, we need to maintain the hypoxic culture condition of CPCs in vitro to maximize the chondrogenic capacity and therapeutic efficacy of CPCs. Furthermore, several studies have demonstrated that hypoxia effectively enhance the therapeutic effects of MSCs, as hypoxic condition can increase the production of regulatory factors in MSCs [ 24 , 26 , 27 ]. In this work, we found hypoxia-preconditioned CGMs showed an enhanced chondrogenic ability and chondro-protective effects in vitro , compared to the normoxia-pretreated CGMs group.…”

“…Oxygen concentration is known to be an important external factor which can regulate the proliferation, differentiation, and self-renewal of mesenchymal stem cells (MSCs) and other stem cells [ 24 ]. It has been reported that hypoxia pretreatment of MSCs is a beneficial strategy to promote cell survival, proliferation, growth factors secretion, and tissue regeneration [ [25] , [26] , [27] ]. Recent studies have showed that hypoxic preconditioning enhanced the function of MSCs, which significantly promoted cartilage repair and alleviate OA progression [ 28 , 29 ].…”

Melatonin-pretreated human umbilical cord mesenchymal stem cells improved endometrium regeneration and fertility recovery through macrophage immunomodulation in rats with intrauterine adhesions

“…We constructed a co-culture system of HucMSCs and macrophages according to the previous studies [39, 40]. The specific scheme was as follows: HucMSCs were pretreated with 10 µ M melatonin for 48 h to achieve 90% fusion and washed three times with PBS to eliminate the effect of melatonin.…”