Hypoxia Up-regulated Angiogenin and Down-regulated Vascular Cell Adhesion Molecule-i Expression and Secretion in Human Placental Trophoblasts

Rajashekhar, G.; Loganath, A.; Roy, A.C.; Chong, Samuel S.; Wong, Yin Kwan

doi:10.1016/j.jsgi.2005.02.010

Cited by 27 publications

(20 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Expression of ANG may be up-regulated in response to hypoxia within the tumor. A significant increase in the secretion and mRNA expression of ANG from both term placental explants and trophoblast cultures subjected to hypoxia in vitro was observed [21] . One study showed that ANG expression in invasive carcinomas is significantly positively correlated with HIF-1 alpha and the HIF-1 alpha target gene DEC-1 [22] .…”

Section: Discussionmentioning

confidence: 96%

Increased expression of angiogenin in gastric carcinoma in correlation with tumor angiogenesis and proliferation

Chen¹,

Zhang²,

Chen³

et al. 2006

WJG

View full text Add to dashboard Cite

AIM:To investigate the implication of angiogenin (ANG) in the neovascularizaton and growth of human gastric carcinoma (HGC).METHODS: ANG mRNA expression in HGC specimens obtained by surgical resection from patients with HGC were examined by RT-PCR. ANG, Ki-67, VEGF protein expression and microvessel density (MVD) in HGC specimens were detected by immunohistochemistry. RESULTS:RT-PCR showed significantly higher ANG mRNA expression (0.482 ± 0.094) in HGC tissues than in the surrounding nontumorous tissues (0.276 ± 0.019, P = 0.03). MVD within tumorous tissues increased significantly with ANG mRNA expression (r = 0.380, P = 0.001) and ANG protein expression (P < 0.01). The ANG expression levels of cancer tissues were positively correlated with VEGF (P < 0.01) and the proliferation index of cancer cells (P < 0.01). www.wjgnet.com well-moderately differentiated adenocarcinoma, and 41 patients had poorly-undifferentiated adenocarcinoma. Fourteen patients were node-negative; 46 had first station lymph node metastasis, and 8 had second or distant lymph node metastasis. Twelve tumors were TNM Ⅰ, 25 tumors were TNM Ⅱ, 20 tumors were TNM Ⅲ, and 11 tumors were TNM Ⅳ. Methods RNA extraction:Specimens were taken within 30 min after resection, and were stored at -80℃. RNA was extracted from HGC tissue and corresponding nontumorous gastric tissue using Trizol extraction reagent kit (Gibco BRL, USA), according to the instructions of the manual. Concentration and purity of RNA were determined by spectrophotometric method. Integrity of RNA was determined by electrophoresis.RT-PCR and analysis of the products: ANG primers were synthesized by the Bioasia Biotechnology Company using the method as described previously [10] . Each tube contained 5 × buffer 5 μL, 10 mmol/L dNTP 0.5 μL, DTT 1.25 μL, primers (sense, antisense) 4 μL, RNA template 1 μg, mix-enzyme 0.5 μL, DEPC solution, with a total volume of 25 μL (Titan One Tube RT-PCR system, Roche, Ger many). RT-PCR reaction condition is as follows: at 58℃ for reverse transcription for 30 min, 94℃ incubation for 2 min to terminate reverse transcription, followed by 30 cycles at 94℃ for 30 s, at 52℃ for 30 s, and at 72℃ for 60 s; With a final extension at 72℃ for 7 min. RT-PCRs were run in a Bio-rad thermocycler. The results were analysed in a Bio-rad GelDoc-1000 system, relative to the levels of β actin as the control. Immunohistochemistry: ANG multiclonal antibody 1:25 was purchased from American Santa Cruz Co. VEGF-C multiclonal antibody (ready to use) was provided by American Zymed Co. Monoclonal antibody of CD34 (QBEnd/10, ready to use) and monoclonal antibody of Ki-67 (MIB-1, 1:50) were supplied by American NeoMarkers Co. All operations were done according to the instructions of the manufacturers. Positive specimens were used as positive controls and PBS in substitution of the first antibody was used as a negative control at the same time. ANG protein expression [8] : Cytoplasm of gastric carcinoma cells was stained brown. According to the staining intensity of the tum...

show abstract

Section: Discussionmentioning

confidence: 96%

Increased expression of angiogenin in gastric carcinoma in correlation with tumor angiogenesis and proliferation

Chen¹,

Zhang²,

Chen³

et al. 2006

WJG

View full text Add to dashboard Cite

show abstract

“…The milieu of the trophoblast cells is characterized by hypoxia [18]. In addition, because vascularization is quite poor at the beginning of implantation, nutrient deprivation is not inconceivable.…”

Section: Discussionmentioning

confidence: 99%

HLA-G expression is induced in Epstein-Barr virus–transformed B-cell lines by culture conditions

et al. 2007

View full text Add to dashboard Cite

“…CRL-1584), which has been used as the model for placenta in previous studies 22–25 , was obtained from the Global Bioresource Center™ (http://www.atcc.org) and cultured in 10% MEM media (modified Eagle’s medium, GIBCO, USA; with 10% fetal bovine serum and antibiotics). The cultures were maintained at 37°C in an atmosphere of 5% of CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Systemic inflammatory stimulation by microparticles derived from hypoxic trophoblast as a model for inflammatory response in preeclampsia

Lee¹,

Romero²,

Lee³

et al. 2012

American Journal of Obstetrics and Gynecology

View full text Add to dashboard Cite

OBJECTIVE To determine whether trophoblast-derived microparticles can induce different inflammatory responses of the peripheral blood mononuclear cells (PBMCs) depending upon the state of trophoblast when the microparticles are generated. STUDY DESIGN A trophoblast-derived cell line (ATCC No. CRL-1584) was cultured under normal or hypoxic conditions. Microparticles were isolated from the cell culture supernatants (MP_C: microparticles from normal trophoblast; MP_H: microparticles from hypoxic trophoblast). PBMCs were cultured alone or co-cultured with either MP_C or MP_H. RESULTS 1) After 48 hours, the PBMCs co-cultured with MP_C released higher concentrations of IL-6 than PBMCs cultured alone; 2) The PBMCs co-cultured with MP_H showed higher concentration of IL-6 and TNF-a than PBMCs co-cultured with PM_C, after 24 hours and 48 hours. CONCLUSION More intense and rapid inflammatory response of PBMCs was observed with MP_H than with MP_C. This difference might explain the exaggerated systemic inflammatory response as a result of placental hypoxia in preeclampsia.

show abstract

Hypoxia Up-regulated Angiogenin and Down-regulated Vascular Cell Adhesion Molecule-i Expression and Secretion in Human Placental Trophoblasts

Cited by 27 publications

References 54 publications

Increased expression of angiogenin in gastric carcinoma in correlation with tumor angiogenesis and proliferation

Increased expression of angiogenin in gastric carcinoma in correlation with tumor angiogenesis and proliferation

HLA-G expression is induced in Epstein-Barr virus–transformed B-cell lines by culture conditions

Systemic inflammatory stimulation by microparticles derived from hypoxic trophoblast as a model for inflammatory response in preeclampsia

Contact Info

Product

Resources

About