Hypoxic Cytotoxicity of Chlorpromazine and the Modification of Radiation Response in<i>E. Coli</i>B/r

The toxicities of three phenothiazines, promazine, chlorpromazine, and trifluoperazine, towards cells of a mouse fibrosarcoma were quantified by means of an in vitro assay of clonogenic cell survival. For all three drugs cell kill was proportional to the amount of drug injected. Following injection of equimolar (0.2 mM/kg) amounts, cell survival was progressively reduced for a period of at least 48 h. On the basis of cell survival at 48 h after administration the ranking of the drugs for cytotoxicity, in ascending order, was trifluoperazine, chlorpromazine, promazine. A period of acute hypoxia prior to processing of the tumor did not enhance the toxicity of any of the drugs, and no change in the size of the hypoxic fraction of the tumor was seen 24 h after the injection of chlorpromazine. On this basis it was concluded that there was no evidence of enhanced toxicity of drugs for either chronically or acutely hypoxic tumor cells. A reduction in the number of clonogenic tumor cells per gram of tumor was largely the result of a fall in the number of viable cells recovered from the tumor. The plating efficiency of surviving cells remained constant or was only slightly depressed.

show abstract

Temperature dependent modification of radiosensitivity following hypoxic cytocidal action of chlorpromazine

Shenoy

Singh

1985

Radiat Environ Biophys

View full text Add to dashboard Cite

The hypoxic cytotoxic effect of chlorpromazine towards E. coli B/r was observed to be dependent on temperature of incubation and irradiation. Increasing the temperature of incubation from ambient to 37 degrees C followed by irradiation revealed that the organisms showed radiosensitivity of a magnitude which was two to three times the oxygen effect. The possible mechanisms of this effect are discussed.

show abstract

Hypoxic Cytotoxicity of Chlorpromazine and the Modification of Radiation Response inE. ColiB/r

Cited by 7 publications

References 15 publications

Inhibition of Cultured Human Tumor Cell Oxygen Utilization by Chlorpromazine

Inhibition of Cultured Human Tumor Cell Oxygen Utilization by Chlorpromazine

In vivo toxicity of phenothiazines to cells of a transplantable tumor

Temperature dependent modification of radiosensitivity following hypoxic cytocidal action of chlorpromazine

Contact Info

Product

Resources

About