Hypoxic Microenvironment Reconstruction with Synergistic Biofunctional Ions Promotes Diabetic Wound Healing

Chen, Xiaochen; Zhang, Liyan; Chai, Wenwen; Tian, Pengfei; Kim, Jua; Ding, Jingxin; Zhang, Hao; Liu, Chunyu; Wang, Deping; Cui, Xu; Pan, Haobo

doi:10.1002/adhm.202301984

Cited by 14 publications

(2 citation statements)

References 60 publications

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“…This demonstrates favorable in vitro biosafety (Figure S17). Previous studies have shown that certain inorganic metal ions, such as Zn 2+ , Fe 2+ , Cu 2+ , Ca 2+ , SiO 4 4– , and PO 4 3– , can enhance the migration of normal fibroblast cells, which is important for wound healing both in vitro and in vivo . − To evaluate the impact of Zn-ASO NPs on mouse embryonic fibroblast cells (NIH-3T3) migration, we performed a scratch experiment. As shown in Figure A, all groups had similar wound widths at the initial time point.…”

Section: Resultsmentioning

confidence: 99%

Coordination-Driven Self-Assembly of Metal Ion–Antisense Oligonucleotide Nanohybrids for Chronic Bacterial Infection Therapy

Li,

Zhang,

Wan

et al. 2024

ACS Appl. Mater. Interfaces

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Bacterial infection poses a significant challenge to wound healing and skin regeneration, leading to substantial economic burdens on patients and society. Therefore, it is crucial to promptly explore and develop effective methodologies for bacterial infections. Herein, we propose a novel approach for synthesizing nanostructures based on antisense oligonucleotides (ASOs) through the coordination-driven self-assembly of Zn 2+ with ASO molecules. This approach aims to provide effective synergistic therapy for chronic wound infections caused by Staphylococcus aureus (S. aureus). The resulting hybrid nanoparticles successfully preserve the structural integrity and biological functionalities of ASOs, demonstrating excellent ASO encapsulation efficiency and bioaccessibility. In vitro antibacterial experiments reveal that Zn-ASO NPs exhibit antimicrobial properties against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis. This antibacterial ability is attributed to the high concentration of metal zinc ions and the generation of high levels of reactive oxygen species. Additionally, the ftsZ-ASO effectively inhibits the expression of the ftsZ gene, further enhancing the antimicrobial effect. In vivo antibacterial assays demonstrate that the Zn-ASO NPs promote optimal skin wound healing and exhibit favorable biocompatibility against S. aureus infections, resulting in a residual infected area of less than 8%. This combined antibacterial strategy, which integrates antisense gene therapy and metal-coordination-directed self-assembly, not only achieves synergistic and augmented antibacterial outcomes but also expands the horizons of ASO coordination chemistry. Moreover, it addresses the gap in the antimicrobial application of metal-coordination ASO self-assembly, thereby advancing the field of ASO-based therapeutic approaches.

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Section: Resultsmentioning

confidence: 99%

Coordination-Driven Self-Assembly of Metal Ion–Antisense Oligonucleotide Nanohybrids for Chronic Bacterial Infection Therapy

Li,

Zhang,

Wan

et al. 2024

ACS Appl. Mater. Interfaces

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“…Li et al developed a pH‐responsive hydrogel based on a natural polymer loaded with insulin to be used as a bioactive dressing for enhancing diabetic wound healing 168 . Healing of foot ulcers of diabetic people is especially difficult, because of the acidic and hyperglycemic microenvironment 169 . A hydrogel was synthesized from n‐carboxyethyl chitosan ( N ‐chitosan), hyaluronic acid‐aldehyde (HA‐ALD), and adipic acid dihydrazide (ADH) with acyl hydrazone, a pH‐sensitive reversible dynamic bond.…”

Section: Non‐linear Functional Polymers Containing Cleavable Bondsmentioning

confidence: 99%

Non‐linear functional polymers containing selective/cleavable bonds: Synthesis and their biomedical applications

Rahimi,

Gałęziewska,

Jerczyński

et al. 2024

Polymers for Advanced Techs

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The growing interest in functional materials has resulted in the emergence of smart polymers, demonstrating practical performance in various applications especially for biomedical purposes. The properties of functional polymers are mainly determined by the presence of functional groups that differ from those in the main chains. While some polymers are naturally active and considered functional, others require modification for enhanced impact and functionality. Many functional polymers typically have a linear backbone, but very recently the attention has been shifted towards those with specific topologies and architecture, for example, bottlebrushes, stars, dendritic polymers, and gels. Over the past few years, there has been a rising emphasis on integrating selective bonds into complex polymer structures to enhance chain scission in single macromolecules. This review captures the most recent and promising approaches to the design of non‐linear functional polymers containing selective/cleavable bonds and discusses the potential of these materials for biomedical applications.

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Calcium Peroxide‐Based Hydrogel Patch with Sustainable Oxygenation for Diabetic Wound Healing

Li,

Lu,

Yang

et al. 2024

Adv Healthcare Materials

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Nonhealing diabetic wounds are predominantly attributed to the inhibition of angiogenesis, re‐epithelialization, and extracellular matrix synthesis caused by hypoxia. Although oxygen therapy has demonstrated efficacy in promoting healing, its therapeutic impact remains suboptimal due to unsustainable oxygenation. Here we propose an oxygen‐releasing hydrogel patch embedded with polyethylene glycol‐modified calcium peroxide microparticles, which sustainably releases oxygen for 7 days without requiring any supplementary conditions. The released oxygen effectively promotes cell migration and angiogenesis under hypoxic conditions as validated in vitro. The in vivo tests in diabetic mice models show that the sustainably released oxygen significantly facilitates the synthesis of extracellular matrix, induces angiogenesis, and decreases the expression of inflammatory cytokines, achieving a diabetic wound healing rate of 84.2% on day 7, outperforming the existing oxygen‐releasing approaches. Moreover, the proposed hydrogel patch is designed with porous, soft, antibacterial, biodegradable, and storage stability for 15 days. The proposed hydrogel patch is expected to be promising in clinics treating diabetic wounds.This article is protected by copyright. All rights reserved

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Hypoxic Microenvironment Reconstruction with Synergistic Biofunctional Ions Promotes Diabetic Wound Healing

Cited by 14 publications

References 60 publications

Coordination-Driven Self-Assembly of Metal Ion–Antisense Oligonucleotide Nanohybrids for Chronic Bacterial Infection Therapy

Coordination-Driven Self-Assembly of Metal Ion–Antisense Oligonucleotide Nanohybrids for Chronic Bacterial Infection Therapy

Non‐linear functional polymers containing selective/cleavable bonds: Synthesis and their biomedical applications

Calcium Peroxide‐Based Hydrogel Patch with Sustainable Oxygenation for Diabetic Wound Healing

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