Hypusination Maintains Intestinal Homeostasis and Prevents Colitis and Carcinogenesis by Enhancing Aldehyde Detoxification

Gobert, Alain P.; Smith, Thaddeus M.; Latour, Yvonne L.; Asim, Mohammad; Barry, Daniel P.; Allaman, Margaret M.; Williams, Kamery J.; McNamara, Kara M.; Delgado, Alberto; Short, Sarah P.; Mirmira, Raghavendra G.; Rose, Kristie L.; Schey, Kevin L.; Zagol-Ikapitte, Irène; Coleman, Jeremy S.; Boutaud, Olivier; Zhao, Shilin; Piazuelo, M. Blanca; Washington, Mary Kay; Coburn, Lori A.; Wilson, Keith T.

doi:10.1053/j.gastro.2023.05.041

Cited by 9 publications

(19 citation statements)

References 56 publications

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“…For the colitis model, 8-12 wk-old mice were treated for 5 days with 4 % dextran sulfate sodium (DSS; TdB Labs) and then maintained on regular water until euthanasia at day 10 [ 12 , 13 , 27 ]. For the model of CAC, mice received one intraperitoneal injection of 12.5 mg/kg azoxymethane (AOM; Millipore) followed by 3 cycles of 4 % DSS for 4 days each, beginning at day 5, 26, and 47 [ 13 , 27 ]. Mice were euthanized at day 56.…”

Section: Methodsmentioning

confidence: 99%

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Analysis of the effect of hypusination in myeloid cells on colitis and colitis-associated cancer

Gobert,

Finley,

Asim

et al. 2024

Heliyon

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Section: Methodsmentioning

confidence: 99%

“…After euthanasia, colons were harvested, measured, weighed, dissected, and Swiss-rolled for histology. In the CAC model, tumors were counted and measured in two dimensions in the distal colon with electronic calipers [ 13 , 27 , 28 ]. Tumor burden corresponds to the sum of areas of all tumors.…”

Section: Methodsmentioning

confidence: 99%

Analysis of the effect of hypusination in myeloid cells on colitis and colitis-associated cancer

Gobert,

Finley,

Asim

et al. 2024

Heliyon

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“… 129 In addition to mimicking primary tissue characteristics, the utilization of tumor organoids enables the investigation of how different gene deletions or mutations directly impact stem cell proliferation or tumor cell apoptosis to further regulate the tumor occurrence and development. 42 , 130 , 131 , 132 , 133 Besides, organoids models are also employed to elucidate the interactions between different cellular components within the tumor microenvironment, 134 , 135 , 136 which leads to an indirect impact on facilitating tumor progression. 137 , 138 , 139 Organoids models also enable the detection of alterations in gene expression and epigenetic modifications during precancerous stages.…”

Section: Organoids Models In Clinic: Gi Disease Simulation and Treatmentmentioning

confidence: 99%

“…Organoids models representing varying degrees of disease severity can be employed to investigate disease progression 149 and facilitate the validation of distinct gene deletions or mutations in disease development. 130 , 138 While the exact causes of many GI inflammatory diseases remain unclear, factors such as genetics, immune system dysfunction, environmental triggers, and dietary habits are believed to play significant roles.…”

Section: Organoids Models In Clinic: Gi Disease Simulation and Treatmentmentioning

confidence: 99%

Organoids in gastrointestinal diseases: from bench to clinic

Wang,

Guo,

Zhang

et al. 2024

MedComm

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The etiology of gastrointestinal (GI) diseases is intricate and multifactorial, encompassing complex interactions between genetic predisposition and gut microbiota. The cell fate change, immune function regulation, and microenvironment composition in diseased tissues are governed by microorganisms and mutated genes either independently or through synergistic interactions. A comprehensive understanding of GI disease etiology is imperative for developing precise prevention and treatment strategies. However, the existing models used for studying the microenvironment in GI diseases—whether cancer cell lines or mouse models—exhibit significant limitations, which leads to the prosperity of organoids models. This review first describes the development history of organoids models, followed by a detailed demonstration of organoids application from bench to clinic. As for bench utilization, we present a layer‐by‐layer elucidation of organoid simulation on host–microbial interactions, as well as the application in molecular mechanism analysis. As for clinical adhibition, we provide a generalized interpretation of organoid application in GI disease simulation from inflammatory disorders to malignancy diseases, as well as in GI disease treatment including drug screening, immunotherapy, and microbial‐targeting and screening treatment. This review draws a comprehensive and systematical depiction of organoids models, providing a novel insight into the utilization of organoids models from bench to clinic.

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“…In addition, they found that simultaneous inhibition of ODC and eIF5A can downregulate MYC expression and decrease colon cancer cell growth [105]. Gobert et al demonstrated that spermidine supplementation can increase eIF5A hypusination in intestinal epithelial cells to prevent the development of colon cancer [106]. Spermidine can promote autophagy and target eIF5A hypusination to regulate the expression of autophagyrelated proteins [49,51].…”

Section: Other Treatments For Ovarian Cancermentioning

confidence: 99%

Polyamines in Ovarian Aging and Disease

Kang,

Wang,

et al. 2023

IJMS

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Ovarian aging and disease-related decline in fertility are challenging medical and economic issues with an increasing prevalence. Polyamines are a class of polycationic alkylamines widely distributed in mammals. They are small molecules essential for cell growth and development. Polyamines alleviate ovarian aging through various biological processes, including reproductive hormone synthesis, cell metabolism, programmed cell death, etc. However, an abnormal increase in polyamine levels can lead to ovarian damage and promote the development of ovarian disease. Therefore, polyamines have long been considered potential therapeutic targets for aging and disease, but their regulatory roles in the ovary deserve further investigation. This review discusses the mechanisms by which polyamines ameliorate human ovarian aging and disease through different biological processes, such as autophagy and oxidative stress, to develop safe and effective polyamine targeted therapy strategies for ovarian aging and the diseases.

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Hypusination Maintains Intestinal Homeostasis and Prevents Colitis and Carcinogenesis by Enhancing Aldehyde Detoxification

Cited by 9 publications

References 56 publications

Analysis of the effect of hypusination in myeloid cells on colitis and colitis-associated cancer

Analysis of the effect of hypusination in myeloid cells on colitis and colitis-associated cancer

Organoids in gastrointestinal diseases: from bench to clinic

Polyamines in Ovarian Aging and Disease

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