Hyunsoonleella sp. HU1-3 Increased the Biomass of Ulva fasciata

Abstract

Green algae are photosynthetic organisms and play an important role in coastal environment. The microbial community on the surface of green algae has an effect on the health and nutrition of the host. However, few species of epiphytic microbiota have been reported to play a role in promoting the growth of algae. In this study, 16S rDNA sequencing was used to study the changes of microbial composition on the surface of Ulva fasciata at different growth stages. Some growth promoting bacteria were identified. The possible growth-promoting behavior of the strains were verified by co-culture of pure bacteria obtained from the surface of U. fasciata with its sterile host. Among the identified species, a new bacterial species, Hyunsoonleella sp. HU1-3 (belonging to the family Flavobacteriaceae) significantly promoted the growth of U. fasciata. The results also showed that there were many genes involved in the synthesis of growth hormone and cytokinin in the genome of Hyunsoonleella sp. HU1-3. This study identified the bacterium Hyunsoonleella sp. HU1-3 for the first time, in which this bacterium has strong growth-promoting effects on U. fasciata. Our findings not only provide insights on the establishment of the surface microbiota of U. fasciata, but also indicate that Hyunsoonleella sp. HU1-3 is one of the important species to promote the growth of U. fasciata.

Functional assembly of surface microbiota of Ulva fasciata improves nutrient absorption efficiency and growth

“…The speci c effects of the SynCom2 of these four of these strains on the growth of U. fasciata were tested by co-culture experiment. Interestingly, H.ulvae HU1-3 alone can signi cantly promoted U.fasciata biomass accumulation in another study [45] but SynCom of ve strains including HU1-3 did not increase the biomass. The SynCom strains of 29 strains had almost the same effect as the 28 strains after removing H.ulvae HU1-3 (results not shown), We selected a mixture of 29 strains (SynCom1) as positive control to demonstrate the effectiveness of the synthetic method.…”

“…Although this was not the highest in network analysis, based on the changes of bacterial abundance under different nitrogen concentrations (Fig. 1) and the species of pure bacteria were obtained [13]. Therefore, we choose Rhodobacteraceae and Flavobacteriaceae as the basis of synchronization, and their evolutionary relationship is shown in Fig.…”

“…Changes in composition and structure of microorganisms affect the interaction between plants and speci c microbial members. Therefore, we can regulate the bene cial growth of certain aspects of plants by increasing the number of functional microbiota in the community [13]. On the other hand, it can reduce the number of functional microorganisms in the community to inhibit the growth of target functions of host plants, and solve environmental problems such as green tide.…”

Applications of Ulva Biomass and Strategies to Improve Its Yield and Composition: A Perspective for Ulva Aquaculture

“…While studies have identified the bacteria required for Ulva development [ 1 , 150 , 151 ], studies identifying specific bacteria influencing the growth of mature thallus and the biochemical composition of the biomass are still lacking. To date, a limited number of studies have attempted to demonstrate that certain bacteria can promote Ulva growth [ 167 , 168 ] and can affect the biochemical composition of Ulva [ 116 ]. Further, examination of the molecular mechanisms driving Ulva : microbial interactions is still limited.…”

Exploring the impact of microbial manipulation on the early development of kelp (Saccharina latissima) using an ecological core microbiome framework

“…In sustainable agriculture, addition of plant-growth promoting bacteria has been shown to reduce the need for chemical fertilizers in terrestrial agricultures (21). Similarly, addition of specific bacterial taxa can increase growth rates of microalgae (22,23) and the macroalga Ulva (24), and many bacteria associated with marine algae produce the plant growth hormone Indole-3-acetic acid (IAA) (25)(26)(27). Bacterial inoculants are also being developed in finfish and shellfish aquaculture to protect against pathogens and increase growth rates (28)(29)(30), and in corals are a strategy for increasing tolerance to high temperature stress (31).…”