Hzf-3 expression in the amygdala after establishment of conditioned taste aversion

Ge, Hong You; Chiesa, Ricardo; Ortı́z, Sandra Peña de

doi:10.1016/s0306-4522(03)00265-3

Cited by 11 publications

(23 citation statements)

References 13 publications

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“…As in previous studies (Ge et al 2003), animals were subjected to a CTA protocol using a 0.1% dextrose solution as the conditioned stimulus (CS) and an intraperitoneal (ip) injection of lithium chloride (LiCl) as the unconditioned stimulus (US). We first examined the effects of ara-CTP, infused bilaterally into the cerebral ventricles 30 min prior to acquisition of CTA, on LTM tested at 24 and 72 h after training (Fig.…”

Section: Ara-ctp Interferes With Ltm Of Ctamentioning

confidence: 99%

“…The CTA training was done as previously described by us (Ge et al 2003). To assess the effectiveness of the infusion pump system and to get the animals used to receiving icv infusions, we subjected each animal to bilateral infusions (5 min at 0.25 µL/min) of vehicle (50% dymethylsulfoxide + 50% of 0.9% saline or 0.9% saline for the ara-CTP/ara-C and anisomycin studies, respectively) on the day prior to conditioning.…”

Section: Behavioral Training and Drug Infusionsmentioning

confidence: 99%

“…The next day, animals were randomly separated into two groups that received ara-CTP (Sigma) at 1 mM working concentration, ara-C (Sigma) at either 0.01 or 1 mM working concentrations, 240 µg anisomycin (Sigma), prepared as described (Manahan-Vaughan et al 2000), or vehicle infusions (see above) 30 min prior to training. Animals were then presented with the CS (a 0.1% dextrose solution) using the bottle presentation method (Ge et al 2003), instead of plain water as in previous days and allowed to drink for 10 min. Forty minutes later, the animals received an ip injection of LiCl (100 mg/kg), the US.…”

Section: Behavioral Training and Drug Infusionsmentioning

confidence: 99%

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The Antimetabolite ara-CTP Blocks Long-Term Memory of Conditioned Taste Aversion

Wang

Ren²,

Perez³

et al. 2003

Learn. Mem.

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We examined the hypothesis that processes related to DNA recombination and repair are involved in learning and memory. Rats received intracerebroventricular (icv) infusions of the antimetabolite 1-beta-D-arabinofuranosylcytosine triphosphate (ara-CTP) or its precursor cytosine arabinoside (ara-C) 30 min prior to conditioned taste aversion (CTA) training. Both ara-CTP and ara-C caused significant impairments in long-term memory (LTM) of CTA. Control experiments indicate that the effect of ara-CTP on CTA memory is related to interference with learning. Furthermore, as it was previously demonstrated for the protein synthesis inhibitor anisomycin, ara-CTP had no effect on CTA memory when it was injected 1 h after training. Importantly, although both ara-CTP and anisomycin significantly blocked LTM in the task, short-term memory (STM) measured 1 h after training was not affected by either of the drugs. Finally, ara-CTP had no effect on in vitro transcription, but it did effectively block nonhomologous DNA end joining (NHEJ) activity of brain protein extracts. We suggest that DNA ligase-mediated DNA recombination and repair processes are necessary for the expression of LTM in the brain.

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Section: Ara-ctp Interferes With Ltm Of Ctamentioning

confidence: 99%

Section: Behavioral Training and Drug Infusionsmentioning

confidence: 99%

Section: Behavioral Training and Drug Infusionsmentioning

confidence: 99%

See 1 more Smart Citation

The Antimetabolite ara-CTP Blocks Long-Term Memory of Conditioned Taste Aversion

Wang

Ren²,

Perez³

et al. 2003

Learn. Mem.

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“…Frozen coronal sections were cut from unfixed tissue (25 m thick) in a cryostat at Ϫ20°C. Sections were pretreated as described previously (Ge et al, 2003). The primary anti-rabbit c-Fos polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA) was used at a 1:100 dilution, and the goat anti-rabbit biotinylated secondary antibody was diluted at 9:1000.…”

Section: Introductionmentioning

confidence: 99%

Consolidation of Fear Extinction Requires Protein Synthesis in the Medial Prefrontal Cortex

Santini¹,

Ge²,

Ren³

et al. 2004

J. Neurosci.

440

322

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Extinction of conditioned fear is thought to form a long-term memory of safety, but the neural mechanisms are poorly understood. Consolidation of extinction learning in other paradigms requires protein synthesis, but the involvement of protein synthesis in extinction of conditioned fear remains unclear. Here, we show that rats infused intraventricularly with the protein synthesis inhibitor anisomycin extinguished normally within a session but were unable to recall extinction the following day. Anisomycin-treated rats showed no savings in the rate of re-learning of extinction, consistent with amnesia for extinction training. The identical effect was observed when anisomycin was microinfused into the medial prefrontal cortex (mPFC) but not the insular cortex. Furthermore, we observed that extinction training increased c-Fos levels in the mPFC but not in the insular cortex, consistent with extinction-induced gene expression in the mPFC. These findings extend previous lesion and unit-recording data by demonstrating that the mPFC is a critical storage site for extinction memory, rather than simply a pathway for expression of extinction. Understanding consolidation of fear extinction could lead to new treatments for anxiety disorders in which fear extinction is thought to be compromised.

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“…The selective involvement of the amygdala in CTA has also been analysed in other ways in animal models. There are studies of receptor expression during taste aversion learning [58,59], studies of the c-Fos expression [60] and other genes [61] in the amygdala, studies of receptors blockade of the amygdala [62] and numerous studies using brain lesions [63], all in the CTA paradigm. For example, possible changes of the leptin receptor expression in the basolateral amygdala in relation to CTA acquisition have been analyzed [59].…”

Section: Amygdaloid Nuclei and Acquisition Of Ctamentioning

confidence: 99%

Amygdala and Taste Learning

Molero‐Chamizo¹

2012

The Amygdala - A Discrete Multitasking Manager

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Hzf-3 expression in the amygdala after establishment of conditioned taste aversion

Cited by 11 publications

References 13 publications

The Antimetabolite ara-CTP Blocks Long-Term Memory of Conditioned Taste Aversion

The Antimetabolite ara-CTP Blocks Long-Term Memory of Conditioned Taste Aversion

Consolidation of Fear Extinction Requires Protein Synthesis in the Medial Prefrontal Cortex

Amygdala and Taste Learning

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