Abarema cochliacarposExtract Decreases the Inflammatory Process and Skeletal Muscle Injury Induced byBothrops leucurusVenom

Abstract

Snakebites are a public health problem, especially in tropical countries. However, treatment with antivenom has limited effectiveness against venoms' local effects. Here, we investigated the ability of Abarema cochliacarpos hydroethanolic extract (EAc) to protect mice against injection of Bothrops leucurus venom. Swiss mice received perimuscular venom injection and were subsequently treated orally with EAc in different doses. Treatment with EAc 100, 200, and 400 mg/kg reduced the edema induced by B. leucurus in 1%, 13%, and 39%, respectively. Although lower doses showed no antihypernociceptive effect in the Von Frey test, the higher dose significantly reduced hyperalgesia induced by the venom. Antimyotoxic activity of EAc was also observed by microscopy assessment, with treated muscles presenting preserved structures, decreased edema, and inflammatory infiltrate as compared to untreated ones. Finally, on the rotarod test, the treated mice showed better motor function, once muscle fibers were preserved and there were less edema and pain. Treated mice could stand four times more time on the rotating rod than untreated ones. Our results have shown that EAc presented relevant activities against injection of B. leucurus venom in mice, suggesting that it can be considered as an adjuvant in the treatment of envenomation.

Snakebite epidemiology and health-seeking behavior in Akonolinga health district, Cameroon: Cross-sectional study

“…The application of the very popular black-stone has been proven ineffective because insufficiently specific to venom proteins, although the absorbing capacity of some types of these "snake stones" has been observed [11,12]. Regarding any benefit of herbal traditional treatments, extensive research suggested encouraging local effects of many plants [13][14][15][16][17] but never when they are used in the raw manner in which they are used in communities [18].…”

Healing effect of the microemulsion enriched with hydroalcoholic extract of Abarema cochliacarpa (Gomes) Barneby J. W. Grimes (Fabaceae)

“…It is noteworthy that the 10% MHMF showed a strong antioxidant behavior, with a percentage of inhibition above 90% (Table 1), which reaffirms the possibility of being active in the reduction of inflammation since antioxidants have a positive correlation with the anti-inflammatory process (Oliveira et al, 2013). Furthermore, previous research by Da Silva et al (2011) and Saturnino-Oliveira et al (2014) showed the effectiveness of A. cochliacarpa in the anti-inflammatory action. At 14 days, it was observed that the three groups showed a similar pattern of CWR and granulation area (p <0.05) ( Figure 5).…”

Pharmacological and toxicological aspects of barbatimão (Abarema cochliacarpos (GOMES) Barneby & J.W. Grimes)

“…Activation of vascular endothelial growth factor (VEGF) and heat shock protein (HSP70) and COX-2 inhibition appear to be involved in cell proliferation, healing and tissue injury regeneration (da Silva, Sánchez-Fidalgo, et al, 2010). The myoprotective effect of the hydroethanolic extract of A. cochliacarpos in Swiss mice was evaluated after injecting the venom of B. leucurus (Saturnino- Oliveira et al, 2014). The myoprotective activity was observed in the microscopic evaluation, at which the treated muscles showed preserved structures, decreasing edema and inflammatory infiltrate compared with untreated animals.…”