<i>Acer okamotoanum</i> Inhibit the Hydrogen Peroxide-Induced Oxidative Stress in C6 Glial Cells

Choi, Seok Reyol; Kim, Ji Hyun; Quilantang, Norman G.; Lee, Sang‐Hyun; Cho, Eun Ju

doi:10.20307/nps.2018.24.3.148

Cited by 4 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sense and antisense primers of respective genes which specific for murine are mentioned in Table 1. Polymerase chain reaction (PCR) was performed by QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) under these conditions: denaturation at 95 • C for 10 min, followed by 45 amplification cycles at 95 • C for 3 s, and annealing at the appropriate temperature for 30 s. The relative gene expression levels were calculated using the ∆∆Cq method with normalization to β-actin as the reference gene [29]. Table 1.…”

Section: Quantitative Real-time Reverse Transcription Polymerase Chaimentioning

confidence: 99%

Effect of Herbal Formulation on Immune Response Enhancement in RAW 264.7 Macrophages

Trinh

Park

et al. 2020

Biomolecules

View full text Add to dashboard Cite

Immune response is a necessary self-defense mechanism that protects the host from infectious organisms. Many medicinal plants are popularly used in Asian folk medicine to increase body resistance. An herbal formulation named KM1608 was prepared from three medicinal plants: Saussurea lappa, Terminalia chebula, and Zingiber officinale. In this study, we evaluated the immune stimulatory effect of KM1608 on RAW 264.7 murine macrophages. Network pharmacological analyses were used to predict potential immune response pathways of major compounds from KM1608. The cytotoxicity and immuno-stimulating effect of KM1608 were determined using cell viability and nitric oxide assays. The underlying mechanism of immunomodulatory activity was evaluated by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) of pro-inflammatory cytokines. The results of network pharmacological analysis suggested that major compounds from KM1608 possess anticancer potential via immune signaling pathways. After treatment with KM1608 at 25–100 µg/mL for 24 h, the level of nitric oxide was increased in the dose-dependent manner. The results of quantitative real-time PCR showed that KM1608 stimulates the expression of immune cytokines (interferon (IFN)-α, -β, IL-1β, -6, IL-10, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2)) in macrophages. KM1608 extract is a potential agent for immune response enhancement.

show abstract

Section: Quantitative Real-time Reverse Transcription Polymerase Chaimentioning

confidence: 99%

Effect of Herbal Formulation on Immune Response Enhancement in RAW 264.7 Macrophages

Trinh

Park

et al. 2020

Biomolecules

View full text Add to dashboard Cite

show abstract

“…Intracellular ROS levels were measured using 2 ,7 -dichlorofluorescin diacetate (H2DCFDA; Sigma) [32]. Cells were exposed to 5 mM glutamate for 8 h and then incubated with 10 µM H2DCFDA for 30 min.…”

Section: Measurement Of Intracellular Rosmentioning

confidence: 99%

Neuroprotective Effects of Tetrahydrocurcumin against Glutamate-Induced Oxidative Stress in Hippocampal HT22 Cells

et al. 2019

View full text Add to dashboard Cite

In the central nervous system, glutamate is a major excitable neurotransmitter responsible for many cellular functions. However, excessive levels of glutamate induce neuronal cell death via oxidative stress during acute brain injuries as well as chronic neurodegenerative diseases. The present study was conducted to examine the effect of tetrahydrocurcumin (THC), a major secondary metabolite of curcumin, and its possible mechanism against glutamate-induced cell death. We prepared THC using curcumin isolated from Curcuma longa (turmeric) and demonstrated the protective effect of THC against glutamate-induced oxidative stress in HT22 cells. THC abrogated glutamate-induced HT22 cell death and showed a strong antioxidant effect. THC also significantly reduced intracellular calcium ion increased by glutamate. Additionally, THC significantly reduced the accumulation of intracellular oxidative stress induced by glutamate. Furthermore, THC significantly diminished apoptotic cell death indicated by annexin V-positive in HT22 cells. Western blot analysis indicated that the phosphorylation of mitogen-activated protein kinases including c-Jun N-terminal kinase, extracellular signal-related kinases 1/2, and p38 by glutamate was significantly diminished by treatment with THC. In conclusion, THC is a potent neuroprotectant against glutamate-induced neuronal cell death by inhibiting the accumulation of oxidative stress and phosphorylation of mitogen-activated protein kinases.Molecules 2020, 25, 144 2 of 10 neurodegenerative diseases [3,4]. Earlier studies showed that an excessive release of glutamate in the extracellular region provoked the accumulation of intracellular ROS by depletion of intracellular glutathione levels through blocking cysteine uptake [5]. The presence of an antioxidant, such as flavonoids or N-acetylcysteine, strongly prevented ROS-induced neuronal cell death [6,7].The use of natural antioxidants for scavenging free radicals and maintaining homeostasis contributes to alleviating neuronal dysfunction [8]. Typically, natural compounds are multiple-target molecules found mainly in microorganisms and plants and exhibit strong antioxidant activity [8,9]. Phenolic compounds from natural sources also exhibit various beneficial effects in cancer, inflammation, and neurodegenerative disorders [9]. This broad spectrum of biological or pharmacological activities has made phytochemicals suitable candidates for treating multifactorial diseases, such as neurodegenerative diseases [10,11]. However, there are also several concerns regarding their poor bioavailability, stability and safety, which must be resolved before effective therapeutics can be developed.Mitogen-activated protein kinases (MAPKs), known as a family of serine/threonine protein kinases, are well-identified biological molecules involved in glutamate-induced oxidative neuronal cell death. MAPKs are related to multiple cellular functions including differentiation, proliferation, cell survival, inflammation, and cell death [12]. The presence of excessive glutam...

show abstract

Protective Role of Natural Products in Glioblastoma Multiforme: A Focus on Nitric Oxide Pathway

Afshari

Mollazadeh

Mohtashami

et al. 2020

CMC

View full text Add to dashboard Cite

: In spite of therapeutic modalities such as surgical resection, chemotherapy and radiotherapy, glioblastoma multiforme (GBM) remains an incurable fatal disease. This necessitates further therapeutic options that could enhance the efficacy of existing modalities. Nitric oxide (NO), a short-lived small molecule, has been revealed to play a crucial role in the pathophysiology of GBM. Several studies have demonstrated that NO is involved in apoptosis, metastasis, cellular proliferation, angiogenesis, invasion and many other processes implicated in GBM pathobiology. Herein, we elaborate on the role of NO as a therapeutic target in GBM and discuss some natural products affecting the NO signaling pathway.

show abstract

Acer okamotoanum Inhibit the Hydrogen Peroxide-Induced Oxidative Stress in C6 Glial Cells

Cited by 4 publications

References 25 publications

Effect of Herbal Formulation on Immune Response Enhancement in RAW 264.7 Macrophages

Effect of Herbal Formulation on Immune Response Enhancement in RAW 264.7 Macrophages

Neuroprotective Effects of Tetrahydrocurcumin against Glutamate-Induced Oxidative Stress in Hippocampal HT22 Cells

Protective Role of Natural Products in Glioblastoma Multiforme: A Focus on Nitric Oxide Pathway

Contact Info

Product

Resources

About