Aceriphyllum rossii Extract and Its Active Compounds, Quercetin and Kaempferol Inhibit IgE-mediated Mast Cell Activation and Passive Cutaneous Anaphylaxis

Abstract

Aceriphyllum rossii contains an abundant source of natural flavonoids with potential antioxidant, anticancer and anti-inflammatory properties. However, the effect of A. rossii extract (ARE) on immunoglobulin E(IgE)-mediated allergic responses remains unknown. In the present study, the effects of ARE and its active compounds, quercetin and kaempferol, on IgE-mediated rat basophilic leukemia mast cell activation and passive cutaneous anaphylaxis (PCA) were investigated. ARE, quercetin, and kaempferol inhibited secretion of β-hexosaminidase and histamine, and reduced the production and mRNA expression of interleukin-4 and tumor necrosis factor-α. ARE also decreased the production of prostaglandin E2 and leukotriene B4 and expression of cyclooxygenase 2 and 5-lipoxygenase. Furthermore, ARE, quercetin, and kaempferol inhibited IgE-mediated phosphorylation of Syk, phospholipase Cγ, protein kinase C (PKC)μ, and the mitogen-activated protein kinases, extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase. ARE, quercetin, and kaempferol markedly suppressed mast cell-dependent PCA in IgE-sensitized mice. These results indicate that ARE and its active constituents, quercetin and kaempferol, may be a useful therapy for immediate-type hypersensitivity.

Kaempferol suppresses lipid accumulation by inhibiting early adipogenesis in 3T3-L1 cells and zebrafish

“…2,3 In cancer cell lines, kaempferol inhibits cell growth and angiogenesis, and thereby induces apoptosis. [7][8][9][10][11][12] It has also been shown that kaempferol negatively regulates adipogenesis in 3T3-L1 cells. 6 In addition, numerous studies suggest that kaempferol has pharmacological effects including anti-oxidant, anti-inflammatory, anti-microbial, neuroprotective, anti-diabetic, anti-osteoporotic, and anti-allergic activities.…”

Citrus Tachibana Leaf Extract Mitigates Symptoms of Food Allergy by Inhibiting Th2‐Associated Responses

“…Several lines of evidence also suggest that kaempferol suppresses Th1 and Th2 responses and the production of neutrophil‐related chemokines that are increased by LPS treatment (Huang and others ). Moreover, kaempferol protects against mast cell degranulation by inhibiting the releases of β‐hexosaminidase and histamines, although attenuating mRNA expression levels of TNF‐α and IL‐4, which collectively contribute to suppress passive cutaneous anaphylaxis (Kim and others ). Lee and others () also have demonstrated that kaempferol mitigates IgE‐induced allergic inflammation in intestinal cell models, such as the RBL‐2H3 and Caco‐2 cell lines, and also suppresses IL‐4 and TNF‐α release by suppressing extracellular signal‐regulated kinases (ERK) activation.…”

“…In addition to kaempferol, the immune‐modulatory role of quercetin has also been studied. Quercetin has been shown to decrease mRNA expression of TNF‐α and IL‐4 by suppressing the phosphorylation of spleen tyrosine kinase (Syk), phospholipase C (PLC)γ, protein kinase C, and mitogen‐activated protein kinases (MAPK) in IgE‐sensitized RBL‐2H3 cells, which are used as a model of intestinal mucosa mast cells (Kim and others ). These collectively suggest that quercetin and kaempferol can be potential therapeutic agents for preventing or treating IgE‐mediated allergic diseases at least in part by regulating Th2‐driven responses.…”

Melanogenesis Inhibitors from the Endophytic Fungus Aspergillus amstelodami

“…The anti-allergic activity of the isolates was investigated in RBL-2H3 at 100 μM using quercetin as a standard anti-allergic compound (Figure 4). [40] In the same context, the ration of hexosaminidase to cell viability was calculated (Figure 4). Compounds 6 and 11 significantly decreased β-hexosaminidase release as compared to control with no significant cytotoxicity to cells.…”