<i>Acinetobacter baumannii</i>in Southern Croatia: clonal lineages, biofilm formation, and resistance patterns

Kaliterna, Vanja; Kaliterna, Mariano; Hrenović, Jasna; Barišić, Zvonimir; Tonkić, Marija; Goić-Barišić, Ivana

doi:10.3109/23744235.2015.1078906

Cited by 20 publications

(18 citation statements)

References 31 publications

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“…Statistically stronger biofilm formation at solid-liquid and air-liquid interfaces was confirmed for antibiotics-sensitive isolates as compared to MDR isolates. This observation is in accordance with statements published for clinical isolates of A. baumannii that isolates sensitive to antibiotics form stronger biofilm (Kaliterna et al, 2015;Perez et al, 2015;Qi et al, 2016). Biofilm protects sensitive isolates from the harmful effect of antibiotics, while MDR isolates have already developed mechanisms to protect themselves from antibiotics and therefore do not tend to assemble cells in biofilm.…”

Section: Swarming and Twitching Surface Motilitysupporting

confidence: 78%

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Virulence Factors of Acinetobacter baumannii Environmental Isolates and Their Inhibition by Natural Zeolite

Dekić¹,

Hrenović²,

Hunjak³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Section: Swarming and Twitching Surface Motilitysupporting

confidence: 78%

“…The ability to form biofilm in vitro was tested via the crystal violet assay (Kaliterna et al, 2015). Overnight bacterial culture was diluted in nutrient broth (Biolife) to an absorbance of 0.1 at 600nm (OD 600 ).…”

Section: Biofilm Formationmentioning

confidence: 99%

Virulence Factors of Acinetobacter baumannii Environmental Isolates and Their Inhibition by Natural Zeolite

Dekić¹,

Hrenović²,

Hunjak³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…In a study by King et al , the top biofilm producers were sensitive to almost every antibiotic tested (ampicillin/sulbactam, ciprofloxacin, gentamicin, imipenem, piperacillin/tazobactam, trimethoprim/sulfamethoxazole, tigecycline), while the lowest producers were multidrug resistant. In a Croatian study, the majority of resistant clinical isolates did not form a biofilm .…”

Section: Discussionmentioning

confidence: 99%

Acinetobacter baumannii isolated from hospital‐acquired infection: biofilm production and drug susceptibility

Krzyściak

Chmielarczyk

Pobiega

et al. 2017

APMIS

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Acinetobacter baumannii cause opportunistic nosocomial infections and is often multidrug resistant. It has ability to form biofilm. The possession of drug resistance mechanism and ability of biofilm formation seems to be the different way to enhancement of viability in stressful environment. In this study, we evaluate relation between these two factors. The biofilm formation was investigated in M63 medium with casein in microtiter plates, and the drug susceptibility was performed by disk diffusion methods. We found that 80-98% strains formed a biofilm. Strains showing sensitivity to amikacin and tobramycin from ICU produced more biofilm than strains showing resistance to these antibiotics. Ceftazidime-sensitive strains formed a smaller biofilm than resistant. The logistic regression shows association between drug resistance and strains originating from ICU. In case of ceftazidime, strong biofilm formation and descending from ICU reduced the likelihood of drug sensitivity. For other drugs such as aminoglycosides, fluoroquinolones, trimethoprim/sulfamethoxazole, and tetracycline, we found opposite relation (but it was not statistically significance). However, generally it seems that strong biofilm producers from ICUs are often more susceptible to antibiotics. This situation can be explained by the fact that bacteria protected in biofilm do not need mechanisms responsible for resistance of planktonic cells.

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“…In another study, 58% and 29% of the isolates belonged to international clones 2 and 1, respectively (32). In the study conducted by Kaliterena et al, in Croatia, 51% of the A. baumannii species belonged to class 1 and 27% to class 2 international clone, and the others belonged to other clones (33).…”

Section: Discussionmentioning

confidence: 99%

Clinical Isolates of Acinetobacter baumannii From Tehran Hospitals: Pulsed-field Gel Electrophoresis Characterization, Clonal Lineages, Antibiotic Susceptibility, and Biofilm-forming Ability

Dehbalaei

Peerayeh

Taherikalani

et al. 2017

Jundishapur J Microbiol

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Background: Acinetobacter baumannii is known as a potential pathogen in hospitals and is responsible for the dramatic increase in carbapenem resistance in Iran in the recent years. Objectives: The current study aimed at determining the genetic association of the isolates by the pulsed-field gel electrophoresis (PFGE) technique, identify international clones, evaluate biofilm formation ability and its relationship with antibiotic resistance. Methods: In the current study, a total of 48 A. baumannii isolates were collected from 2 hospitals in Tehran, Iran, from 2010 to 2012. Isolates were subjected to antimicrobial susceptibility testing, determination of carbapenemase encoding genes, biofilm formation, and genetic relationships analysis. Results:The obtained results demonstrated that the rate of resistance to carbapenem, meropenem, imipenem, and doripenem was 76%. The carbapenemase-encoding gene blaOXA-23-like was found in 32 isolates, while blaOXA-40-like (blaOXA-24-like), blaOXA-58-like, blaVIM-type and blaIMP-type were found in 11, 1, 19. and 5 isolates, respectively. When the lineage of the isolates was evaluated by the multiplex polymerase chain reaction (PCR), it was found that 28 isolates belonged to group 1 and 8 isolates to group 2. None of the isolates belonged to group 3. Twelve isolates could not be typed by this method. The study findings interestingly demonstrated that 13 isolates showed no biofilm formation. Data of biofilm formation also demonstrated that 28, 4, and 3 remaining isolates had weak, moderate, and strong biofilm formation, respectively. The pulsed field gel electrophoresis result revealed 11 unique clones. Conclusions: International clone 1 was the most commonly identified clone in the current study. This clone was mostly associated with blaOXA-23-like gene; therefore, 64% of the isolates in this clone possessed blaOXA-23-like gene.

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Acinetobacter baumanniiin Southern Croatia: clonal lineages, biofilm formation, and resistance patterns

Abstract: Clinical isolates of A. baumannii from respiratory samples in ICUs with a particular susceptibility pattern are more prone to form biofilm.

Cited by 20 publications

References 31 publications

Virulence Factors of Acinetobacter baumannii Environmental Isolates and Their Inhibition by Natural Zeolite

Virulence Factors of Acinetobacter baumannii Environmental Isolates and Their Inhibition by Natural Zeolite

Acinetobacter baumannii isolated from hospital‐acquired infection: biofilm production and drug susceptibility

Clinical Isolates of Acinetobacter baumannii From Tehran Hospitals: Pulsed-field Gel Electrophoresis Characterization, Clonal Lineages, Antibiotic Susceptibility, and Biofilm-forming Ability

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